Skip to main content
SpringerLink
Log in

Protection induced in BALB/c mice by the high-molecular-mass (hMM) fraction of Paracoccidioides brasiliensis

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

Paracoccidioidomycosis (PCM) is a granulomatous disease caused by a dimorphic fungus, Paracoccidioides brasiliensis. The present study investigated the protective activity of the P. brasiliensis high-molecular-mass (hMM) fraction (~380 kDa) in experimental murine PCM. In the first step, lymphocyte proliferation and production of IFNγ (but not IL-4) were observed in “in vitro” spleen cells (from female BALB/c mice infected (i.v.) with P. brasiliensis) that were stimulated with hMM fractions. In the second step, female BALB/c mice were previously immunized (s.c.) with hMM fraction (25 μg/protein = F-25 and 50 μg/protein = F-50), and the colony-forming units (CFU) of the lung and spleen, the histopathological characteristics of the granulomatous lesions, and plasmatic gp43 soluble antigens and anti-hMM IgG levels were analyzed at 28 and 56 days after infection. The lung and liver CFU were lower in mice previously immunized with the hMM fraction (P < 0.05). The granulomatous lesions revealed a greater degree of compaction and organization, with no dissemination of the fungus to other organs. Lower soluble antigen levels (P < 0.05) and higher IgG anti-hMM fraction (P < 0.05) were observed in immunized groups. The results for CFU, histopathology and antigenemia suggest that the hMM fraction has a protective effect in experimental paracoccidioidomycosis in BALB/c mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Reference

  1. Brummer E, Castañeda E and Restrepo A (1993). Paracoccidioidomycosis an update. Clin Microbiol Rev 6: 89–117

    PubMed  CAS  Google Scholar 

  2. McEwen JG, Garcia AM, Ortiz BL, Botero S and Restrepo A (1995). Arch Med Res 26: 305–306

    PubMed  CAS  Google Scholar 

  3. Franco MF, Mendes RP, Rezkallah-Iwasso M and Montenegro MR (1989). Paracoccidioidomycosis. Baillièrés Clin Trop Med Commun Dis 4: 185–219

    Google Scholar 

  4. Wanke B and Londero AT (1994). Epidemiology and paracoccidioidomycosis infection. In: Franco, M, Lacaz, CS, Restrepo-Moreno, A and Del-Negro, G (eds) 109–120. CRC Press, Inc, Boca Raton, Florida

    Google Scholar 

  5. Oliveira SJ, Mamoni RL, Musatti CC, Papaiordanou PMO and Blotta MHSL (2002). Cytokines and lymphocyte proliferation in juvenile and adult forms of paracoccidioidomycosis: comparison with infected and non-infected controls. Microbes Infect 4: 139–144

    Article  PubMed  CAS  Google Scholar 

  6. Kashino SS, Fazioli RA, Cafalli-Favati C, Meloni-Bruneri LH, Vaz CA, Burger E, Singer LM and Calich VL (2000). Resistance to Paracoccidioides brasiliensis infection is linked to a preferential Th1 immune response, whereas susceptibility is associated with absence of IFN-gamma production. J Interferon Cytokine Res 20: 89–97

    Article  PubMed  CAS  Google Scholar 

  7. Calich VLG and Kashino SS (1998). Cytokines produced by susceptible and resistant mice in the course of Paracoccidioides brasiliensis infection. Braz J Med Biol Res 31: 615–623

    Article  PubMed  CAS  Google Scholar 

  8. Graybill JR, Tollemar J, Torres-Rodríguez JM, Walsh TJ, Roilides E and Farmaki E (2000). Antifungal compounds: Controversies, queries and conclusions. Med Mycol 38(supplement I): 323–333

    PubMed  CAS  Google Scholar 

  9. Gubbins PO and Amsden JR (2005). Drug–drug interactions of antifungal agents and implications for patient care. Expert Opin Pharmacother 6(13): 2231–2243

    Article  PubMed  CAS  Google Scholar 

  10. Taborda CP, Juliano MA, Puccia R, Franco MV and Travassos LR (1998). Mapping of the T-cell epítope in the major 43-kilodalton glycoprotein of Paracoccidioides brasiliensis which induces a Th-1 response protective against fungal infection in Balb/c mice. Infect Immun 6: 786–793

    Google Scholar 

  11. Pinto AR, Puccia R, Diniz SN, Franco MF and Travassos LR (2000). DNA-based vaccination against murine paracoccidioidomycosis using the gp43 gene from Paracoccidioides brasiliensi. Vaccine 18: 3050–3058

    Article  PubMed  CAS  Google Scholar 

  12. Diniz SN, Reis BS, Zouain CS, Leite MF and Goes AM (2004). Protective immunity induced in mice by F0 and FII antigens purified from Paracoccidioides brasiliensis. Vaccine 22: 485–492

    Article  PubMed  CAS  Google Scholar 

  13. Grosso DM, Almeida SR, Mariano M and Lopes JD (2003). Characterization of gp70 and anti-gp70 monoclonal antibodies in Paracoccidioides brasiliensis pathogenesis. Infect Immun 71: 6534–6542

    Article  CAS  Google Scholar 

  14. Puccia R, Schenkman S, Gorin PAJ and Travassos LR (1986). Exocellular components of Paracoccidioides brasiliensis: Identification of a specific antigen. Infect Immun 53: 199–206

    PubMed  CAS  Google Scholar 

  15. Márquez AS, Vicentini P, Ono MA, Watanabe MAE, Camargo ZP and Itano EN (2005). J Clin Labor Anal 19: 199–204

    Article  CAS  Google Scholar 

  16. Camargo ZP, Taborda CP, Rodrigues EG and Travassos LR (1991). The use of cell-free antigens of Paracoccidioides brasiliensis in serological tests. J Med Vet Mycol 29: 31–38

    PubMed  CAS  Google Scholar 

  17. Camargo ZP, Unterkircher C, Campoy SP and Travassos LR (1988). Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests. J Clin Microbiol 26: 2147–2151

    PubMed  Google Scholar 

  18. Miura CSN, Estevão D, Lopes JD and Itano EN (2001). Levels of specific antigen (gp43), specific antibodies, and antigen-antibody complexes in saliva and serum of paracoccidioidomycosis patients. Med Mycol 39(5): 423–428

    PubMed  CAS  Google Scholar 

  19. Coutinho ZF, Silva D, Lazéra M, Petri V, Oliveira RM, Sabroza PC and Wanke B (2002). Paracoccidiodomycosis mortality in Brazil (1980–1995). Cad Saúde Pública, Rio de Janeiro 18(5): 1441–1454

    Google Scholar 

  20. Defavrei J, Rez J, Rezkallah-Iwasso MT and Franco MF (1982). Experimental pulmonary paracoccidioidomycosis in mice: Morphology and correlation of lesions with humoral and cellular immune response. Mycopathologia 77(1): 3–11

    Article  Google Scholar 

  21. Defaveri J, Martin LC and Franco M (1989). Histological and ultrastructural study of the inflammation evoked by Paracoccidioides brasiliensis antigen in previously immunized mice. Mycopathologia 105(1): 53–58

    Article  PubMed  CAS  Google Scholar 

  22. Soares AMVC, Peraçoli MTS and Santos RR (2002). Correlation among immune response, morphogenesis of granulomatous reaction and spleen lymphoid structure in murine experimental paracoccidioidomycosis. Med Mycol 38: 317–377

    Google Scholar 

  23. Polonelli L, Casadevall A, Han Y, Bernardis F, Kirkland TN, Matthews RC, Adrian D, Boccanera M, Burnie JP, Cassone A, Conti S, Cutler JE, Frazzi R, Gregory C, Hodgetts S, Illidge C, Magliani W, Rigg G and Santoni G (2000). The efficacy of acquired humoral and cellular immunity in the prevention and therapy of experimental fungal infections. Med Mycol 38: 281–292

    PubMed  Google Scholar 

  24. Singer-Vernes LM, Caldeira CB, Burger E and Calish LG (1993). Experimental murine paracoccidioidomycosis: Relationship among the dissemination of the infection, humoral and cellular immune responses. Clin Exp Immunol 94: 75–79

    Article  Google Scholar 

  25. Benard G, Mendes-Gianinni MT, Juvenale M, Miranda ET and Duarte AJ (1997). Immunosuppression in paracoccidioidomycosis: T cell hyporesponsiveness to two Paracoccidioides brasiliensis glycoproteins that elicit strong humoral immune response. J Infect Dis 175: 1263–1267

    Article  PubMed  CAS  Google Scholar 

  26. Cacere CR, Romano CC, Mendes-Giannini MJS, Duarte AJS and Benard G (2002). The role of apoptosis in the antigen-specific T cell hyporesponsiveness of paracoccidioidomycosis patients. Clin Immunol 105: 215–222

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fundação Araucária, Curitiba, PR, Brazil

    Wander R. Pavanelli

  2. Department of Pathology Science, CCB, State University of Londrina, 86051-990, Londrina, PR, Brazil

    Mari S. Kaminami, Mario A. Ono & Eiko N. Itano

  3. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brasilia, Brazil

    Juliana R. Geres

  4. Research Center of Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan

    Ayako Sano

  5. Department of Biology Science, Paulista State University, Assis, SP, Brazil

    I. C. C. Camargo

Authors
  1. Wander R. Pavanelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mari S. Kaminami
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Juliana R. Geres
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ayako Sano
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mario A. Ono
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. I. C. C. Camargo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Eiko N. Itano
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eiko N. Itano.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pavanelli, W.R., Kaminami, M.S., Geres, J.R. et al. Protection induced in BALB/c mice by the high-molecular-mass (hMM) fraction of Paracoccidioides brasiliensis . Mycopathologia 163, 117–128 (2007). https://doi.org/10.1007/s11046-007-0095-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11046-007-0095-4

Keywords

Search

Navigation