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Validation of an HPLC Analytical Method Coupled to a Multifunctional Clean-up Column for the Determination of Deoxynivalenol

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Abstract

To evaluate a method using a multifunctional clean-up column coupled with high performance liquid chromatography as an official analytical method for the determination of deoxynivalenol in wheat used as food or feed, an inter-laboratory study was performed in 12 laboratories using four naturally contaminated wheat samples and one spiked sample. The relative standard deviations for repeatability (RSDr) and reproducibility (RSDR) of naturally contaminated wheat were in the range 5.8–11.3% and 12.0–20.7%, respectively. The HORRAT was less than 1.0 in each sample. From the spiking test, the recovery rate, RSDr, RSDR and HORRAT value were 100.0%, 11.2%, 10.3% and 0.5, respectively. The limit of quantification is 0.10 mg/kg from the range obtained in a linear calibration. Thus, it should be useful as a sensitive and validated analytical method for the determination of deoxynivalenol in wheat intended for use in food and feed.

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Authors and Affiliations

  1. National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan

    Yoshiko Sugita-Konsihi & Kosuke Takatori

  2. Kobe Institute of Health, Kobe, Japan

    Toshitsugu Tanaka

  3. Tokyo Metropolitan Institute of Public Health, Tokyo, Japan

    Setsuko Tabata

  4. Nagoya City Public Health Research Institute, Nagoya, Japan

    Masahiro Nakajima

  5. Yokohama Quarantine Station, Yokohama, Japan

    Masanori Nouno

  6. Kobe Quarantine Station, Kobe, Japan

    Yoko Nakaie

  7. Hokkaido Institute of Public Health, Hokkaido, Japan

    Takao Chonan & Mitsutoshi Aoyagi

  8. Japan Food Research Laboratories, Osaka branch, Osaka, Japan

    Nobuyuki Kibune

  9. Fertilizer and Feed Inspection Services, Tokyo, Japan

    Kazutoshi Mizuno & Eiichi Ishikuro

  10. Japan Grain Inspection Association, Tokyo, Japan

    Naoki Kanamaru & Masatoshi Minamisawa

  11. Fertilizer and Feed Inspection Services, Sendai Branch, Sendai, Japan

    Norio Aita

  12. National Food Research Institute, Tokyo, Japan

    Masayo Kushiro & Kenji Tanaka

Authors
  1. Yoshiko Sugita-Konsihi
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  2. Toshitsugu Tanaka
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  3. Setsuko Tabata
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  4. Masahiro Nakajima
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  9. Nobuyuki Kibune
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  10. Kazutoshi Mizuno
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  11. Eiichi Ishikuro
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  12. Naoki Kanamaru
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  13. Masatoshi Minamisawa
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  14. Norio Aita
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  15. Masayo Kushiro
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  16. Kenji Tanaka
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  17. Kosuke Takatori
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Correspondence to Yoshiko Sugita-Konsihi.

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Sugita-Konsihi, Y., Tanaka, T., Tabata, S. et al. Validation of an HPLC Analytical Method Coupled to a Multifunctional Clean-up Column for the Determination of Deoxynivalenol. Mycopathologia 161, 239–243 (2006). https://doi.org/10.1007/s11046-006-0260-1

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  • DOI: https://doi.org/10.1007/s11046-006-0260-1

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