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Role of mast cells as IL10 producing cells in paracoccidioidomycosis skin lesions

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Abstract

Recent works have demonstrated that mast cells may have an important role in immunologic reactions and inflammation once they synthesize and secrete many cytokines including IL4, IL5, IL6 and TNF-α. We have conducted research in order to verify if mast cells would participate in the local inflammatory immune response against Paracoccidioides brasiliensis in skin lesions characterized by a Th2 pattern of cytokines. Fifty-nine skin biopsies with previous histopathological diagnosis of paracoccidioidomycosis and immunohistochemical characterization of cytokines present in the inflammatory infiltrate were classified in three groups: group 1 (G1), with compact granuloma and a Th1 pattern of cytokines; group 2 (G2), with loose granuloma and a Th2 pattern of cytokines; group 3 (G3), both kind of granuloma in the same lesion, characterized by cytokines from Th1 and Th2 patterns. Ten biopsies from normal skin were used as control group. Mast cells were visualized and quantified by a toluidine blue/HCl staining and a double immunostaining was performed to detect a co-localization of mast cells and IL10. G2 presented an increased number of mast cells when compared to G1, G3 and control group and we frequently could find mast cells expressing IL10 in G2. The data obtained suggest that mast cells participate in the immune response against P. brasiliensis in skin lesions with loose granuloma and a Th2 pattern of cytokines. Considering these results, mast cells could constitute a source of IL10, contributing to a non-effective response against fungal antigens.

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References

  1. P Bradding ST Holgate (1999) ArticleTitleImmunopathology and human mast cell cytokines Crit Rev Oncol Hematol 31 119–133 Occurrence Handle10.1016/S1040-8428(99)00010-4 Occurrence Handle1:STN:280:DyaK1MzotFWjtw%3D%3D Occurrence Handle10451798

    Article  CAS  PubMed  Google Scholar 

  2. Bradding P, Walls AF, Church MK. Mast cells and basophils: their role in initiating and maintaining inflammatory responses. In: Holgate ST ed. Immunopharmacology of the Respiratory System. New York Academic Press, 1995: 53-4

  3. P Frandji C Oskeritzian F Cacaraci et al. (1993) ArticleTitleAntigen-dependent stimulation by bone-marrow-derived mast cells of MHC class II-restricted T cell hybridoma J Immunol 151 6318–6328 Occurrence Handle1:CAS:528:DyaK2cXivFaksg%3D%3D Occurrence Handle8245470

    CAS  PubMed  Google Scholar 

  4. KS Love RR Lakshmanan JH Butterfield et al. (1996) ArticleTitleIFN-gamma stimulated enhancement of MHC class II antigen expression by the human mast cell line HMC-I Cell Immunol 170 85–90 10.1006/cimm.1996.0137 Occurrence Handle10.1006/cimm.1996.0137 Occurrence Handle1:CAS:528:DyaK28Xjtlaitb0%3D Occurrence Handle8660803

    Article  CAS  PubMed  Google Scholar 

  5. Y Suzumura M Ohasi (1991) ArticleTitleImmunoelectron microscopic localization of HLA-DR antigen on mast cells and vessels in normal and tuberculin-reactive skin Am J Dermatopathol 13 568–574 10.1097/00000372-199113060-00007 Occurrence Handle10.1097/00000372-199113060-00007 Occurrence Handle1:STN:280:DyaK383hsVOktw%3D%3D Occurrence Handle1805651

    Article  CAS  PubMed  Google Scholar 

  6. P Bradding JA Roberts KM Britten et al. (1994) ArticleTitleInterleukin-4, -5 and -6 and tumor necrosis factor-alpha in normal and asthmatic airways: Evidence for the human mast cell as a source of these cytokines Am J Respir Cell Mol Biol 10 471–480 Occurrence Handle1:CAS:528:DyaK2cXktVOhsrw%3D Occurrence Handle8179909

    CAS  PubMed  Google Scholar 

  7. LJ Walsh G Trinchieri HA Waldorf et al. (1991) ArticleTitleHuman dermal mast cells contain and release tumor necrosis factor alpha, which induces endothelial leukocyte adhesion molecule Proc Natl Acad.Sci USA 88 4220–4224 10.1073/pnas.88.10.4220 Occurrence Handle10.1073/pnas.88.10.4220 Occurrence Handle1:CAS:528:DyaK3MXkt1OnsbY%3D Occurrence Handle1709737

    Article  CAS  PubMed  Google Scholar 

  8. L Thompson-Snipes V Dhar MW Bond et al. (1991) ArticleTitleInterleukin 10: A novel stimulatory factor for mast cells and their progenitors J Exp Med 173 507–510 10.1084/jem.173.2.507 Occurrence Handle10.1084/jem.173.2.507 Occurrence Handle1:CAS:528:DyaK3MXnvFyrsg%3D%3D Occurrence Handle1899106

    Article  CAS  PubMed  Google Scholar 

  9. R MalavIya T Ikeda E Ross et al. (1996) ArticleTitleMast cell modulation of neutrophil influx and bacterial clearance at sites of infection through TNF-alpha Nature 381 77–80 10.1038/381077a0 Occurrence Handle10.1038/381077a0 Occurrence Handle1:CAS:528:DyaK28XislCru7g%3D Occurrence Handle8609993

    Article  CAS  PubMed  Google Scholar 

  10. BK Wershil I Castagliuolo C Pothoulakis (1998) ArticleTitleDirect evidence of mast cell involvement in Clostridium difficile toxin A-induced enteritis in mice Gastroenterology 114 956–964 10.1016/S0016-5085(98)70315-4 Occurrence Handle10.1016/S0016-5085(98)70315-4 Occurrence Handle1:CAS:528:DyaK1cXjtVSjs78%3D Occurrence Handle9558284

    Article  CAS  PubMed  Google Scholar 

  11. M Jutel S Klunker M Akdis et al. (2001) ArticleTitleHistamine upregulates Th1 and downregulates Th2 responses due to different patterns of surface histamine 1 and 2 receptor expression Int Arch Allergy Immunol 124 190–192 10.1159/000053707 Occurrence Handle10.1159/000053707 Occurrence Handle1:CAS:528:DC%2BD3MXjt1Squ7w%3D Occurrence Handle11306965

    Article  CAS  PubMed  Google Scholar 

  12. S Muñhoz R Hernãndez-Pando SN Abraham et al. (2003) ArticleTitleMast cell activation by Mycobacterium tuberculosis: Mediator release and role of CD48 J Immunol 170 5590–5596

    Google Scholar 

  13. IJ Elenkov E Webster DA Papanicolaou et al. (1998) ArticleTitleHistamine potently suppresses human IL12 and stimulates IL10 production via H2 receptors J Immunol 161 2586–2593 Occurrence Handle1:CAS:528:DyaK1cXlslamsL4%3D Occurrence Handle9725260

    CAS  PubMed  Google Scholar 

  14. DR Roach AG Bean C Demangel et al. (2002) ArticleTitleTNF regulates chemokine induction essential for cell recruitment, granuloma formation and clearance of mycobacterial infection J Immunol 168 4620–4627 Occurrence Handle1:CAS:528:DC%2BD38Xjt12jtLY%3D Occurrence Handle11971010

    CAS  PubMed  Google Scholar 

  15. S Smith D Liggitt E Jeromsky et al. (2002) ArticleTitleLocal role for tumor necrosis factor-α in the pulmonary inflammatory response to Mycobacterium tuberculosis infection Infect Immun 70 2082–2089 10.1128/IAI.70.4.2082-2089.2002 Occurrence Handle10.1128/IAI.70.4.2082-2089.2002 Occurrence Handle1:CAS:528:DC%2BD38Xitl2ls7o%3D Occurrence Handle11895974

    Article  CAS  PubMed  Google Scholar 

  16. AS Marques (1994) Cutaneous lesions MF Franco CS Lacaz A Retrepo (Eds) et al. Paracoccidioidomycosis CRC Press Bocca Raton 256–269

    Google Scholar 

  17. M Franco MR Montenegro RP Mendes et al. (1987) ArticleTitleParacoccidioidomycosis: A recently proposed classification of its clinical forms Rev Soc Bras Med Trop 20 129–132 Occurrence Handle1:STN:280:DyaL1M7osFOksw%3D%3D Occurrence Handle3507739

    CAS  PubMed  Google Scholar 

  18. C Pagliari MN Sotto (2003) ArticleTitleDendritic cells and pattern of cytokines in paracoccidioidomycosis skin lesions Am J Dermatopathol 25 107–112 10.1097/00000372-200304000-00003 Occurrence Handle10.1097/00000372-200304000-00003 Occurrence Handle12652191

    Article  PubMed  Google Scholar 

  19. SA Shukla R Veerappan JS Whittimore et al. (2006) ArticleTitleMast cell ultrastructure and staining in tissue Methods Mol Biol 315 63–76 Occurrence Handle16110149

    PubMed  Google Scholar 

  20. SC Bischoff G Sellge (2002) ArticleTitleMast cell hyperplasia: Role of cytokines Int Arch Allergy Immunol 127 118–122 10.1159/000048181 Occurrence Handle10.1159/000048181 Occurrence Handle1:CAS:528:DC%2BD38XitlGmtr8%3D Occurrence Handle11919420

    Article  CAS  PubMed  Google Scholar 

  21. SC Bischoff G Sellge A Lorentz et al. (1999) ArticleTitleIL-4 enhances proliferation and mediator release in mature human mast cells Proc Natl Acad Sci USA 96 8080–8085 10.1073/pnas.96.14.8080 Occurrence Handle10.1073/pnas.96.14.8080 Occurrence Handle1:CAS:528:DyaK1MXltVOrsrY%3D Occurrence Handle10393951

    Article  CAS  PubMed  Google Scholar 

  22. H Toru M Eguchi R Matsumoto et al. (1998) ArticleTitleInterleukin-4 promotes the development of tryptase and chymase double-positive human mast cells accompanied by cell maturation Blood 91 187–195 Occurrence Handle1:CAS:528:DyaK1cXms1Gm Occurrence Handle9414284

    CAS  PubMed  Google Scholar 

  23. CC Musatti MT Rezkallah E Mendes et al. (1976) ArticleTitleIn vivo and in vitro evaluation of cell-mediated immunity in patients with paracoccidioidomycosis Cell Immunol 24 365–378 10.1016/0008-8749(76)90220-3 Occurrence Handle10.1016/0008-8749(76)90220-3 Occurrence Handle1:STN:280:DyaE283jtl2htg%3D%3D Occurrence Handle779977

    Article  CAS  PubMed  Google Scholar 

  24. P Bradding IH Feather S Wilson et al. (1993) ArticleTitleImmunolocalization of cytokines in the nasal mucosa of normal and perennial rhinitic subjects. The mast cell as a source of IL4, IL5 and IL6 in human allergic mucosal inflammation J Immunol 151 3853–3865 Occurrence Handle1:CAS:528:DyaK2cXms1yq Occurrence Handle8376806

    CAS  PubMed  Google Scholar 

  25. V Supajatura U Ushio A Nakao et al. (2002) ArticleTitleDifferential response of mast cell Toll-like receptor 2 and 4 in allergy and innate immunity J Clin Invest 109 1351–1359 10.1172/JCI200214704 Occurrence Handle1:CAS:528:DC%2BD38XjvFCls7c%3D Occurrence Handle12021251

    CAS  PubMed  Google Scholar 

  26. A Ozinsky DM Underhill JD Fontenot et al. (2000) ArticleTitleThe repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between Toll-like receptors PNAS 97 13766–13771 10.1073/pnas.250476497 Occurrence Handle10.1073/pnas.250476497 Occurrence Handle1:CAS:528:DC%2BD3cXoslyns7w%3D Occurrence Handle11095740

    Article  CAS  PubMed  Google Scholar 

  27. JW Streilein P Alard H Niizeki (1999) ArticleTitleA new concept of skin-associated lymphoid tissue (SALT): UVB light impaired cutaneous immunity reveals a prominent role for cutaneous nerves Keio J Med 48 22–27 Occurrence Handle1:STN:280:DyaK1M3it1OntQ%3D%3D Occurrence Handle10206015

    CAS  PubMed  Google Scholar 

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  1. University of São Paulo — Medical School, São Paulo, Brazil

    Carla Pagliari, Elaine R. Fernandes, Fernanda Guedes, Cleiton Alves & Mirian N. Sotto

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  1. Carla Pagliari
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  2. Elaine R. Fernandes
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  3. Fernanda Guedes
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  4. Cleiton Alves
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  5. Mirian N. Sotto
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Correspondence to Carla Pagliari.

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Pagliari, C., Fernandes, E.R., Guedes, F. et al. Role of mast cells as IL10 producing cells in paracoccidioidomycosis skin lesions. Mycopathologia 162, 331–335 (2006). https://doi.org/10.1007/s11046-006-0069-y

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  • DOI: https://doi.org/10.1007/s11046-006-0069-y

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