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Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients

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Abstract

Malassezia furfur is lypophilic yeast commonly associate with dermatological disorders. In the present work, we described the isolation of 47 M. furfur strains from three groups of patients: pityriasis versicolor (21 isolates), seborrhoeic dermatitis (15 isolates) and seborrhoeic dermatitis of the HIV positive patients (11 isolates). To investigate the identity of the strains at molecular level, DNA genomic of M. furfur strains were prepared and used to RAPD-PCR analyses. RAPD assay were carried out using two decamer primers and bands pattern generated were analyzed by an Unweighted Pair-Group Method (UPGMA). Dendrogram established a distinct differentiation between M. furfur isolates from pityriasis versicolor and seborrhoeic dermatitis patients with or without AIDS. We concluded that RAPD typing presented a high discriminatory power between strains studied in this work and can be applied in epidemiological investigation of skin disease causing by M. furfur.

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Abbreviations

PV:

pityriasis versicolor

SD:

seborrhoeic dermatitis

SD/HIV:

seborrhoeic dermatitis of HIV positive patients

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Authors and Affiliations

  1. Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069, Cascavel, 85814-110, PR, Brazil

    Rinaldo F. Gandra & Rita C. G. Simão

  2. Departamento de Microbiologia, Seção de Micologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 05508-900, SP, Brazil

    Flávia E. Matsumoto, Bosco C. M. da Silva, Luciana S. Ruiz, Eriques G. da Silva, Walderez Gambale & Claudete R. Paula

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  1. Rinaldo F. Gandra
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  2. Rita C. G. Simão
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  3. Flávia E. Matsumoto
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  4. Bosco C. M. da Silva
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Correspondence to Rinaldo F. Gandra.

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Gandra, R.F., Simão, R.C.G., Matsumoto, F.E. et al. Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients. Mycopathologia 162, 273–280 (2006). https://doi.org/10.1007/s11046-006-0063-4

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