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Effect of pepstatin A on the virulence factors of Candida albicans strains isolated from vaginal environment of patients in three different clinical conditions

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Abstract

The aspartate proteinase inhibitor pepstatin A was used to study a possible correlation among proteinase activity and other virulence factors of Candida albicans strains isolated from the vaginal environment of patients in three different clinical conditions: asympthomatic, vulvovaginal candidiasis (VVC) and recurrent vulvovaginal candidiasis (RVVC). The addition of 1.0 μM pepstatin A did not have any significant effect on hyphae formation, biofilm production and in the cell surface hydrofobicity of isolates in the three different clinical conditions. However, pepstatin A reduced the adherence of C. albicans to vaginal mucosa epithelial cells (53.1, 48.7 and 59.9%, respectively to isolates from asymptomatic, VVC and RVVC patients). This result suggests that the secreted aspartate proteinases (Saps) of this fungal pathogen may have auxiliary roles in cellular adhesion.

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Authors and Affiliations

  1. Division of Medical Mycology, Teaching and Research in Clinical Analysis Laboratory, State University of Maringá, Maringá, Paraná, Brazil

    M. E. L. Consolaro & T. I. E. Svidzinski

  2. Department of Odontology, State University of Maringá, Maringá, Paraná, Brazil

    A. Gasparetto

  3. Department of Biochemistry , State University of Maringá, Maringá, Paraná, 5790, CEP: 87020-900, Brazil

    R. M. Peralta

Authors
  1. M. E. L. Consolaro
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  2. A. Gasparetto
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  3. T. I. E. Svidzinski
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  4. R. M. Peralta
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Correspondence to R. M. Peralta.

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Consolaro, M.E.L., Gasparetto, A., Svidzinski, T.I.E. et al. Effect of pepstatin A on the virulence factors of Candida albicans strains isolated from vaginal environment of patients in three different clinical conditions. Mycopathologia 162, 75–82 (2006). https://doi.org/10.1007/s11046-006-0026-9

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  • DOI: https://doi.org/10.1007/s11046-006-0026-9

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