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Differences in virulence between isolates of feline Sporotrichosis

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Abstract

Sporotrichosis is a chronic subcutaneous mycosis caused by Sporothrix schenckii. This work aimed to evaluate the virulence of two different isolates of S. schenckii from cutaneous (CUT) and systemic (SYS) forms of feline sporotrichosis. A standard inoculum with 2 × 103 yeast cells/ml was prepared from each of the isolates. The experimental infection was carried out with 0.1 ml of the inoculum from both isolates and then injected in the paw pads of Swiss albino mice of groups CUT and SYS. The clinical evolution of the disease and the diameter of the lesion at the inoculated sites were evaluated during nine weeks. Four necropsies were done to collect material from the lesions (p < 0.01). Group CUT demonstrated a more evident clinical evolution of the disease from week two to week five; large lesions in the paw pad on week four (p < 0.01); and a higher incidence of lesions in other parts of the body (p < 0.01) than group SYS (p < 0.01). S. schenckii was isolated from the inoculated site in groups SYS and CUT until days 30 and 45, respectively. Granulomas with yeast cells usually localized in the central area were observed in histopathology sections on days 15 and 30 post-inoculations. Those yeast cells decreased on day 45 being absent on day 62 when tissue repair initiated. The results showed that distinct clinical isolates of S. schenckii cause significant differences in the clinical evolution of sporotrichosis.

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References

  1. KJ Kwon-Chung JE Bennett (1992) Medical mycology Lea & Fibeger Philadelphia

    Google Scholar 

  2. GH Findlay HF Vismer (1986) ArticleTitleStudies in sporotrichosis: fungal morphogenesis and pathogenicity in differing environments Mycopathologia 96 115–122 Occurrence Handle10.1007/BF00436670 Occurrence Handle3796713

    Article  PubMed  Google Scholar 

  3. SA Marques RMP Camargo V Haddad SuffixJr MEA Marques SRVS Franco NS Rocha (1998) ArticleTitleHuman sporotrichosis: transmitted by feline An. Bras. Dermatol. 73 559–562

    Google Scholar 

  4. MO Nobre AP Castro D Caetano LL Souza MCA Meireles L Ferreiro (2001) ArticleTitleRecurrence of sporotrichosis in cats with zoonotic involvement Rev. Iberoam. Micol. 18 137–140 Occurrence Handle15487925

    PubMed  Google Scholar 

  5. TM Schubach A de Oliveira Schubach RS Reis T Cuzzi-Maya TCM Blanco DF Monteiro BM Barros R Brustein RM Zancope-Oliveira PCF Fialho Monteiro B Wanke (2001) ArticleTitleSporothrix schenckii isolated from domestic cats with and without sporotrichosis in Rio de Janeiro, Brazil Mycopathologia 153 83–86 Occurrence Handle10.1023/A:1014449621732

    Article  Google Scholar 

  6. DM Dixon RA Duncan NJ Hurd (1992) ArticleTitleUse of a mouse model to evaluate clinical and environmental isolates of Sporothrix spp. From the largest U.S. epidemic of sporotrichosis J. Clin. Microbiol. 30 951–954 Occurrence Handle1572983

    PubMed  Google Scholar 

  7. KJ Kwon-Chung (1979) ArticleTitleComparison of isolates of Sporothrix schenckii obtained from fixed cutaneous lesions with isolates from other types of lesions J. Infect. Dis. 139 424–431 Occurrence Handle438543

    PubMed  Google Scholar 

  8. R Romero-Martinez M Wheeler A Guerrero-Plata G Rico H Torres-Guerrero (2000) ArticleTitleBiosynthesis and functions of melanin in Sporothrix schenckii Infect. Immun. 68 3696–3703 Occurrence Handle10.1128/IAI.68.6.3696-3703.2000 Occurrence Handle10816530

    Article  PubMed  Google Scholar 

  9. T Tachibana T Matsuyama M Mitsuyama (1998) ArticleTitleCharacteristic infectivity of Sporothrix schenckii to mice depending on routes of infection and inherent fungal pathogenicity Med. Mycol. 36 21–27 Occurrence Handle10.1046/j.1365-280X.1998.00114.x Occurrence Handle9776808

    Article  PubMed  Google Scholar 

  10. LA González de Polania A Alzate N Saravia (1990) ArticleTitleComportamiento experimental del Sporothrix schenckii y la Leishmania mexicana em el hamster Rev. Inst. Med. Trop. 32 319–324

    Google Scholar 

  11. MT Mujica M Agotegaray A Canoba P Delasoie S Hauswirth G Kociziki M Tartabini D Alvarez (1992) ArticleTitleEsporotricose experimental en ratas Rev. Arg. Micologia. 15 7–12

    Google Scholar 

  12. T Tachibana MI Matsuyama M Ito M Mitsuyama (2001) ArticleTitleSporothrix schenckii thermo-intolerant mutants losing fatal visceral infectivity but retaining high cutaneous infectivity Med. Mycol. 39 295–298 Occurrence Handle11446534

    PubMed  Google Scholar 

  13. T Yoshike PC Lei H Komatsuzaki H Ogawa (1993) ArticleTitleAntibody raised against extracellular proteinases of Sporothrix schenckii in S. schenckii inoculated hairless mice Mycopathologia 123 69–73 Occurrence Handle10.1007/BF01365082 Occurrence Handle8264769

    Article  PubMed  Google Scholar 

  14. IZ Carlos MMC Zini DBG Sgarbi J Angluster CS Alviano CL Silva (1994) ArticleTitleDisturbances in the production of inteleukin-1 tumor and necrosis factor in disseminated murine sporotrichosis Mycopathologia 3 189–194 Occurrence Handle10.1007/BF01102920

    Article  Google Scholar 

  15. MBL Barros AO Schubach MCG Galhardo TMP Schubach RS Reis MJ Conceição ACF Valle (2003) ArticleTitleSporotrichosis with widespread cutaneous lesions: report of 24 cases related to transmission by domestic cats in Rio de Janeiro, Brazil Int. J. Dermatol. 42 677–681 Occurrence Handle10.1046/j.1365-4362.2003.01813.x Occurrence Handle12956676

    Article  PubMed  Google Scholar 

  16. J Carvalho SuffixJr JB Caldwell BL Radford AR Feldman (1991) ArticleTitleFeline-transmitted sporotrichosis in the Southwestern United States Western J. Med. 54 462–465

    Google Scholar 

  17. RW Dunstan RF Langham KA Reimann PS Wakenell (1986) ArticleTitleFeline sporotrichosis: a report of five cases with transmission to humans J. Am. Acad. Dermatol 15 37–45 Occurrence Handle3722508

    PubMed  Google Scholar 

  18. CE Larsson MA Gonçalves VC Araujo MLZ Dagli B Correa C Fava-Neto (1989) ArticleTitleEsporotricosis felina: aspectos clínicos e zoonóticos Rev. Inst. Med. Trop. 31 351–358

    Google Scholar 

  19. RN Fleury PR Taborda AK Grupta MS Fujita PS Rosa AC Weckwerth MS Negrão I Bastazini (2001) ArticleTitleZoonotic sporotrichosis. Transmission to humans by infected domestic cat stratching: report of four cases in São Paulo, Brazil Int. J. Dermatol. 40 318–322 Occurrence Handle11575308

    PubMed  Google Scholar 

  20. BS Belknap (1989) ArticleTitleSporotrichosis Dermatol clin. 7 193–202

    Google Scholar 

  21. KW Donadel YD Reinoso JC Oliveira RD Azulay (1993) ArticleTitleEsporotricose: revisão An. Bras. Dermatol. 68 45–52

    Google Scholar 

  22. Souza LL. Sporothrix schenckii: estudo epidemiológico em população de gatos. [Dissertation]. Pelotas, Brazil. Faculdade de Veterinária, Universidade Federal de Pelotas. 2001: 32p

  23. TM Schubach AO de Schubach T Cuzzi-Maya T Okamoto RS Reis PC Monteiro MC Gutierrez-Galhardo B Wanke (2003) ArticleTitlePathology of sporotrichosis in 10 cats in Rio de Janeiro Vet. Rec. 152 172–175 Occurrence Handle12622288

    PubMed  Google Scholar 

  24. MB Albornoz M Mendoza ED Torres (1986) ArticleTitleGrowth temperatures of isolates of Sporothrix schenckii from disseminated and fixed cutaneous lesions of sporotrichosis Mycopathologia 95 81–83 Occurrence Handle10.1007/BF00437165 Occurrence Handle3762662

    Article  PubMed  Google Scholar 

  25. R Morris-Jones S Youngchim BL Gomez P Aisen RJ Hay JD Nosanchuk A Casadevall AJ Hamilton (2003) ArticleTitleSynthesis of melanin-like pigments by Sporothrix schenckii in vitro and during mammalian infection Infect. Immun. 71 4026–4033 Occurrence Handle10.1128/IAI.71.7.4026-4033.2003 Occurrence Handle12819091

    Article  PubMed  Google Scholar 

  26. R Tsuboi T Sanada K Takamori H Ogawa (1987) ArticleTitleIsolation and properties of extracellular proteinases from Sporothrix schenckii J. Bacteriology 169 4104–4109

    Google Scholar 

  27. T Yoshiike PC Lei H Komatsuzaki H Ogawa (1993) ArticleTitleAntibody raised against extracellular proteinases of Sporothrix schenckii in S schenckii inoculated hairless mice Mycopathologia 123 69–73 Occurrence Handle10.1007/BF01365082 Occurrence Handle8264769

    Article  PubMed  Google Scholar 

  28. KS Fernandes HL Mathews L Bezerra (1999) ArticleTitleDifferences in virulence of Sporothrix schenckii conidia related to culture conditions and cell-wall components J. Med. Microbiol. 48 195–203 Occurrence Handle9989648

    PubMed  Google Scholar 

  29. IZ Carlos DB Sgarbi MC Placeres (1999) ArticleTitleHost organism defense by a peptide-polysaccharide extracted from the fungus Sporothrix schenckii Mycopathologia 144 9–14 Occurrence Handle10.1023/A:1006964516334

    Article  Google Scholar 

  30. WN Arnold LC Mann KH Sakai RG Garrison PD Coleman (1986) ArticleTitleAcid phosphatases of Sporothrix schenckii J. Gen. Microbiol. 132 3421–3432 Occurrence Handle3655720

    PubMed  Google Scholar 

  31. H Ishizaki (2003) ArticleTitleMitochondrial DNA analysis of Sporothrix schenckii Nippon Ishinkin Gakkai Zasshi 44 155–157 Occurrence Handle12913803

    PubMed  Google Scholar 

  32. H Ishizaki M Kawasaki M Aoki T Matsumoto AA Padhye M Mendoza R Negroni (1998) ArticleTitleMitochondrial DNA analysis of Sporothrix in North and South América Mycopathologia 142 115–118 Occurrence Handle10.1023/A:1006952702947 Occurrence Handle10052160

    Article  PubMed  Google Scholar 

  33. X Liu C Lian L Jin L Na G Yang X Lin (2003) ArticleTitleCharacterization of Sporothrix schenckii by random amplification of polymorphic DNA assay Chin. Méd. J. 116 239–242 Occurrence Handle12775239

    PubMed  Google Scholar 

  34. AC Mesa-Arango (2002) ArticleTitlePhenotyping and genotyping of Sporothrix schenckii isolates according to geographic origin and clinical form of sporotrichosis Clin. Microbiol. 40 3004–3011 Occurrence Handle10.1128/JCM.40.8.3004-3011.2002

    Article  Google Scholar 

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Veterinárias - Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Avemda Bento Gonçalves no. 9090-91540-000, Porto Alegre, RS-Brazil

    Márcia de Oliveira Nobre & Marlete Brum Cleff

  2. Programa de Pós-Graduação em Veterinária - Faculdade de Veterinária, Universidade Federal de Pelotas UFPel, Brazil

    Tatiana de Ávila Antunes & Renata Osório de Faria

  3. Faculdade de Veterinária, UFPel, Brazil

    Cristina Gevehr Fernandes & Mário Carlos Araújo Meireles

  4. Setor de Micologia, Faculdade de Veterinária, UFRGS, Brazil

    Adriana Cunha Muschner & Laerte Ferreiro

Authors
  1. Márcia de Oliveira Nobre
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  2. Tatiana de Ávila Antunes
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  3. Renata Osório de Faria
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  4. Marlete Brum Cleff
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  5. Cristina Gevehr Fernandes
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  6. Adriana Cunha Muschner
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  7. Mário Carlos Araújo Meireles
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  8. Laerte Ferreiro
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Correspondence to Márcia de Oliveira Nobre.

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Nobre, M.d., Antunes, T.d., Faria, R.O. et al. Differences in virulence between isolates of feline Sporotrichosis. Mycopathologia 160, 43–49 (2005). https://doi.org/10.1007/s11046-005-6866-x

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  • DOI: https://doi.org/10.1007/s11046-005-6866-x

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