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Generation and characterization of reduced virulence Fusarium oxysporum f. sp. lycopersici mutants through plasmid-vector insertion

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Abstract

Pathogenicity-impaired mutants, B02 and H15, of Fusarium oxysporum f. sp. lycorpersici (FOL) were obtained using restriction enzyme-mediated integration. Disease severities of Fusarium wilt caused by these mutants were significantly reduced, and their disease development rates were correlated with their colonization rates in tomato vessels. Both B02 and H15 produced significantly smaller amounts of extracellular proteins as well as fusaric acid than the wild-type. Southern blot analyses suggested that B02 and H15 likely contain a single and three copies of transformation vector, respectively. These mutants may thus be useful in isolating genes involved in pathogenicity of FOL.

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Authors and Affiliations

  1. Graduate School of Science and Technology, Chiba University, Yayoicho-1-33, Inage, Chiba-city, Chiba, 263-8522, Japan

    Koji Morita, Soichiro Kimura, Masanori Saito & Hirofumi Shinoyama

  2. Faculty of Horticulture, Chiba University, Matsudo 648, Matsudo-city, Chiba, 271-8510, Japan

    Toshiyuki Usami, Yoshimiki Amemiya & Masahiro Shishido

Authors
  1. Koji Morita
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  2. Soichiro Kimura
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  3. Masanori Saito
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  4. Hirofumi Shinoyama
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  5. Toshiyuki Usami
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  6. Yoshimiki Amemiya
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  7. Masahiro Shishido
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Correspondence to Masahiro Shishido.

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Morita, K., Kimura, S., Saito, M. et al. Generation and characterization of reduced virulence Fusarium oxysporum f. sp. lycopersici mutants through plasmid-vector insertion. Mycopathologia 160, 67–73 (2005). https://doi.org/10.1007/s11046-005-1155-2

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  • DOI: https://doi.org/10.1007/s11046-005-1155-2

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