Skip to main content

Advertisement

SpringerLink
Log in

Virulence Factors and Antifungal Susceptibility of Candida albicans Isolates from Oral Candidosis Patients and Control Individuals

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

Sixty isolates of Candida albicans, 30 obtained from the oral cavity of denture wearers presenting signs of candidosis and 30 obtained from the oral cavity of denture wearers with normal palatal mucosa were assayed for phospholipase and proteinase production, as well as for adherence to buccal epithelial cells. Likewise, susceptibility of the isolates to antifungals was determined by the NCCLS reference method and the E-test method. Proteinase activity was increased among the strains obtained from oral candidosis patients. In contrast, no significant differences between the two groups of isolates were observed in their adherence ability in vitro, in phospholipase production, and susceptibility to antifungal drugs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M Monod M Borg-von Zepelin (2002) ArticleTitleSecreted proteinases and their virulence mechanism of Candida albicans Chem Immunol 81 114–118 Occurrence Handle1:CAS:528:DC%2BD38Xlt1Olurw%3D Occurrence Handle12101999

    CAS  PubMed  Google Scholar 

  2. AS Kantarcioglu A Yucel (2002) ArticleTitlePhospholipase and protease activities in clinical Candida isolates with reference to the sources of strains Mycoses 45 160–165 Occurrence Handle1:CAS:528:DC%2BD38XlvVSlsrc%3D Occurrence Handle12100532

    CAS  PubMed  Google Scholar 

  3. LP Samaranayake JM Raeside TW MacFarlane (1984) ArticleTitleFactors affecting the phospholipase activity of Candida species in vitro J Med Vet Mycol 22 201–207 Occurrence Handle1:CAS:528:DyaL2cXlsVOgtbk%3D

    CAS  Google Scholar 

  4. P Mayser S Laabs K Heuer K Gründer (1996) ArticleTitleDetection of extracellular phospholipase activity in Candida albicans and Rhodotorula rubra Mycopathologia 135 149–155 Occurrence Handle10.1007/BF00632336 Occurrence Handle1:CAS:528:DyaK2sXitlegsrg%3D Occurrence Handle9066156

    Article  CAS  PubMed  Google Scholar 

  5. V Vidotto CY Koga-Ito R Milano B Fianchino J Pontón (1999) ArticleTitleCorrelation between germ-tube production, phospholipase activity and serotype distribution in Candida albicans Rev Iberoam Micol 16 208–210

    Google Scholar 

  6. SR Torres CB Peixoto DB Caldas EB Silva T Aikiti M Nucci M Uzeda (2002) ArticleTitleRelationship between salivary flow rates and Candida counts in subjects with xerostomia Oral Surg Oral Med Oral Pathol Oral Radiol Endodont 93 149–154

    Google Scholar 

  7. LJ Maza J Elguezabal C Prado J Eilacuria I Soler J Pontón (2002) ArticleTitleCandida albicans adherence of the role human saliva Oral Surg Oral Med Oral Pathol Oral Radiol Endodont 94 589–592

    Google Scholar 

  8. RG Nair LP Samaranayake (1996) ArticleTitleThe effect of oral commensal bacteria on Candidal adhesion to human buccal epithelial cells in vitro J Med Microbiol 45 179–185 Occurrence Handle1:STN:280:BymH3cnosFM%3D Occurrence Handle8810944

    CAS  PubMed  Google Scholar 

  9. A Wellmer H Bernhardt (1997) ArticleTitleAdherence on buccal epithelial cells and germ tube formation in the continuous flow culture of clinical Candida albicans isolates Mycoses 40 363–368 Occurrence Handle1:STN:280:DyaK1c7jtFWquw%3D%3D Occurrence Handle9470423

    CAS  PubMed  Google Scholar 

  10. P Sundstrom (2002) ArticleTitleAdhesion in Candida spp Cell Microbiol 4 461–169 Occurrence Handle10.1046/j.1462-5822.2002.00206.x Occurrence Handle1:CAS:528:DC%2BD38XntFajs7s%3D Occurrence Handle12174081

    Article  CAS  PubMed  Google Scholar 

  11. CC Villar H Kashleva A Dongari-Bagtzoglou (2004) ArticleTitleRole of Candida albicans polymorphism in interactions with oral epithelial cells Oral Microbiol Immunol 19 262–269 Occurrence Handle10.1111/j.1399-302X.2004.00150.x Occurrence Handle1:CAS:528:DC%2BD2cXms1Gqsbk%3D Occurrence Handle15209998

    Article  CAS  PubMed  Google Scholar 

  12. K Barrett-Bee Y Hayes RG Wilson J Ryley (1985) ArticleTitleA comparison of phospholipase activity, cellular adherence and pathogenicity in yeasts J Gen Microbiol 31 1217–1221

    Google Scholar 

  13. M Ghannoum K Abu Elteen (1986) ArticleTitleCorrelative relationship between proteinase production, adherence and pathogenicity of various strains of Candida albicans J Med Vet Mycol 24 407–413 Occurrence Handle1:STN:280:BiiD28%2FjvF0%3D Occurrence Handle3537262

    CAS  PubMed  Google Scholar 

  14. VL Bosco EG Bruman AE Cury CR Paula (2003) ArticleTitleYeasts from the oral cavity of children with AIDS: exoenzyme production and antifungal resistance Pesqui Odontol Bras 17 217–222 Occurrence Handle14762498

    PubMed  Google Scholar 

  15. MA Ribeiro AE Mirandal W Gambale CR Paula (2004) ArticleTitlePrevalence and exoenzyme secretion of Candida albicans isolates from oral and vagina mucosas of HIV-infected women Mycopathologia 157 255–261 Occurrence Handle15180151

    PubMed  Google Scholar 

  16. A Borst AC Fluit (2003) ArticleTitleHigh levels of hydrolytic enzymes secreted by Candida albicans isolates involved in respiratory infections J Med Microbiol 52 971–974 Occurrence Handle1:CAS:528:DC%2BD2cXktVGmtbg%3D Occurrence Handle14532341

    CAS  PubMed  Google Scholar 

  17. M Shu NB Ellepola LP Samaranayake (2001) ArticleTitleEffects of two different growth media on the post antifungal effect induced by polyenes on Candida species J Clin Microbiol 39 2732–2735 Occurrence Handle10.1128/JCM.39.7.2732-2735.2001 Occurrence Handle1:CAS:528:DC%2BD3MXlsFSjtrc%3D Occurrence Handle11427609

    Article  CAS  PubMed  Google Scholar 

  18. P Sandvén (1990) ArticleTitleLaboratory identification and sensitivity testing of yeasts isolates Acta Odontol Scand 48 27–36 Occurrence Handle1690944

    PubMed  Google Scholar 

  19. A Polak (1992) ArticleTitleVirulence of a Candida albicans mutants Mycoses 35 9–16 Occurrence Handle1:STN:280:ByyD38vntVY%3D Occurrence Handle1406791

    CAS  PubMed  Google Scholar 

  20. S Aoki S Ito-Kuwa Y Nakamura T Masuhara (1990) ArticleTitleComparative pathogenicity of wild type strains and respiratory mutants of Candida albicans in mice Zentralbl Bakteriol 273 332–343 Occurrence Handle1:STN:280:By6D3c%2FhtF0%3D Occurrence Handle2206203

    CAS  PubMed  Google Scholar 

  21. AB Macura E Tondyra (1989) ArticleTitleInfluence of some carbohydrates and concanavalin A on the adherence of Candida albicans in vitro to buccal epithelial cells Zentralbl Bakteriol 272 196–201 Occurrence Handle1:CAS:528:DyaK3cXlsVyrsLk%3D Occurrence Handle2698625

    CAS  PubMed  Google Scholar 

  22. National Committee for Clinical Laboratory Standards. Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved standard M27-A2. National Committee for Clinical Laboratory Standards. Villanova, PA, 2002.

  23. AB Biodisk. E-test technical guide, no 4: antifungal susceptibility testing of yeasts. AB BIODISK, Solna, Sweden, 1993.

  24. T Närhi A Ainamo J Meurman (1993) ArticleTitleSalivary yeasts, saliva and oral mucosa in the elderly J Den Res 72 1009–1014

    Google Scholar 

  25. S Cardaropoli D Fraia ParticleDi E Menegatti S Aoki V Vidotto (1997) ArticleTitleCorrelation between chlamydosporulation, germ tube, phospholipase and proteinase production in Candida albicans J Med Mycol 7 169–170

    Google Scholar 

  26. B Hube R Rüchel M Monod D Sanglard FC Odds (1988) ArticleTitleFunctional aspects of secreted Candida proteinases Adv Exp Med Biol 436 339–344

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônìo Carlos, 6627, P.O. Box 486, 31270-901, Belo Horizonte, MG, Brazil

    Cristiane Yumi Koga-Ito, Juliana Pereira Lyon & Maria Aparecida de Resende

  2. Laboratorio di Micologia Medica, Sez. Malattie Infettive, Dipartimento Discipline Medico-Chirurgiche, Universitá di Torino, Turin, Italy

    Valerio Vidotto

Authors
  1. Cristiane Yumi Koga-Ito
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juliana Pereira Lyon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Valerio Vidotto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maria Aparecida de Resende
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Koga-Ito, C.Y., Lyon, J.P., Vidotto, V. et al. Virulence Factors and Antifungal Susceptibility of Candida albicans Isolates from Oral Candidosis Patients and Control Individuals. Mycopathologia 161, 219–223 (2006). https://doi.org/10.1007/s11046-005-0001-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11046-005-0001-x

Keywords

Search

Navigation