The inhibition of Candida albicans by selected essential oils and their major components
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Many volatile oils are known to possess antifungal properties and are potentially applicable as antimycotic agents. By studying the efficacy of essential oils against different pathogenic mycetes, we have evaluated the in-vitro inhibiting activity of some essential oils and their main constituents against a strain of Candida albicans. Sixteen commercial essential oils and forty-two pure constituents (alcohols, aldehydes, ketons, phenols and hydrocarbons), were tested by using a semisolid agar antifungal susceptibility (SAAS) method. Gas chromatography/mass spectroscopy analyses of the oils tested were performed. The essential oils of Origanum vulgare, Satureja montana, Mentha piperita, Cinnamomum verum, Cymbopogon flexuosus showed maximum inhibitory activity (MIC = 500 ppm) after 7 days. According to the results of the examination of pure constituents, β-phellandrene proved to be the most interesting component among cyclic monoterpenic hydrocarbons as it showed a strong activity (MIC = 50 ppm). The most active of phenols was carvacrol (MIC 100 ppm). The open-chain alcohol 1-decanol was the most active of alcohols at 50 ppm. Finally, among aldehydes, a strong activity was shown by trans-cynnamaldehyde (MIC 50 ppm).
KeywordsCandida albicans essential oils in-vitro test pure constituents
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- Deans, SG, Subota, KP, Kennedy, AI. 1989Biological activity of plant volatile oils and their constituentsPlanta Med55588Google Scholar
- Stenhagen, E, Abrahamsson, S, McLafferty, FW 1974Registry of mass spectral dataWiley & SonsNew YorkGoogle Scholar
- Massada, Y. 1976Analysis of essential oils by gas chromatography and mass spectrometryJ Wiley & SonsNew YorkGoogle Scholar
- Jennings, W, Shibamoto, T. 1980Qualitative analysis of flavor and fragrance volatiles by glass capillary chromatographyAcademic PressNew YorkGoogle Scholar
- Swigar, AA, Silverstein, RM. 1981MonoterpenesAldrich Chem CompMilwaukeeGoogle Scholar
- Adams, RP. 1995Identification of essential oil components by gas chromatography-mass spectroscopyAllured Publishing CorpCarol Stream, IllinoisGoogle Scholar
- Concha, JM, Moore, LS, Holloway, WJ. 1998Antifungal activity of Melaleuca alternifolia (tea–tree) oil against various Pathogenic OrganismsJ Am Podiatr Med Ass88489492Google Scholar
- Chao, SC, Young, DG, Oberg, CJ. 2000Screening for inhibitory activity of essential oils on selected bacteria, fungi and virusesJEOR12639649Google Scholar
- Cosentino, S, Tuberoso, CIG, Fadda, ME, Pisano, B, Satta, M, Mascia, V, Palmas, F. 1999Attività antimicrobica e composizione chimica di oli essenziali della SardegnaL’Igiene Moderna11214111421Google Scholar