, Volume 159, Issue 1, pp 53–63 | Cite as

Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) as a potential mycoparasite on Sphaerotheca fuliginea (Ascomycotina: Erysiphales)

  • Miloslava KavkováEmail author
  • Vladislav Čurn


Hyphomycete Paecilomyces fumosoroseus that is well known as saprophytic and entomopatogenic fungus was investigated for its mycoparasitism on the cucumber powdery mildew pathogen. Mycoparasitism was documented by using standard bioassay and SEM. Effects of mycoparasitism were evaluated in three types of experiments. Paecilomyces fumosoroseus was applied in the form of graded suspensions into a colony of powdery mildew on a leaf segment. Interaction between both fungi was observed as the percentage of colonized area vs. experimental time. In the second experiment, young cucumber plants were sprayed with a suspension of Paecilomyces fumosoroseus 24 h before inoculation of Sphaerotheca fuliginea. Pre-treatment with P. fumosoroseus reduced development and spreading of powdery mildew infection significantly 15 days post-inoculation in contrast to pre-treatments with sulfur fungicide and distilled water. The development of pure culture powdery mildew under determined experimental conditions was observed and compared with treated variants. In the third experiment, mildewed plants were treated with a suspension of P. fumosoroseus. The control treatments with sulfur fungicide and distilled water were tested. Effects of P. fumosoroseus on the dispersion of powdery mildew during a 21-day period were observed.

P. fumosoroseus suppressed the development and spread of cucumber powdery mildew significantly during the time of the experiment. The mechanical and physical damages and disruptions of vegetative and fruiting structures of powdery mildew were recorded under light microscopy and S.E.M.

Results were concluded in pursuance to differences between the natural behaviour and development of S. fuliginea on cucumber plants treated with P. fumosoroseus and non-treated plants.


cucumber powdery mildew mycoparasitism Paecilomyces fumosoroseus Sphaerotheca fuliginea 


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  1. Samson, R. 1974Paecilomyces and some allied hyphomycetesStudies in Mycology.6143Google Scholar
  2. Samson, RA. 1985Biology of the Verticillium and AschersoniaHussez, N.V. eds. scopes, N.E.A., Biological pest control - the glasshouse experienceCornell University Ithaca PressN.Y.3242Google Scholar
  3. Osborne, LS., Landa, Z. 1992Biological control of whiteflies with entomopathogenic fungiFlorida entomologist.75456471Google Scholar
  4. Bolckmans, K., Sterk, G., Eyal, J., Sels, B., Stepman, W, PreFeRal,  1995Paecilomyces fumosoroseus strain Apopka 97), a new microbial insecticide for the biological control of whiteflies in greenhouses, Mededelingen - Faculteit Landbouwkundige en Toegepaste Biologishe WetenschappenUniversiteit Gent.60707711Google Scholar
  5. Lacey, LA., Kirk, AA., Millar, L., Mercardier, G., Vidal, C. 1999Ovicidal and larvicidal activity of conidia and blastospores of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against Bemisisa argentifolii (Homoptera:Aleyrodidae) with description of a bioassay system allowing prolonged survival of control insectsBiocontrol Sci. Techn.9918Google Scholar
  6. Ellis MB., Ellis JP. Fungi specific to various herbaceous plants. In: Microfungi on land plants. The Richmond Publishing Co. Ltd., 1997: p. 867.Google Scholar
  7. Sundheim, L., Admundsen, T. 1982Fungicide tolerance in the hyperparasite Ampelomyces quisqualis and integrated control of cucumber powdery mildewActa Agri Scand.32349Google Scholar
  8. Hijwegen, T. 1989Effect of culture filtrates seventeen fungicolous fungi on sporulation of cucumber powdery mildewNeth. J. Pl. Path.95598Google Scholar
  9. Hijwegen, T. 1992Biological control of cucumber powdery mildew with Tilletiopsis minor under greenhouse conditionsNeth. J. Pl. Path.98221225Google Scholar
  10. Lebeda, A. 1983The genera and Species spectrum of cucumber powdery mildew in CzechoslovakiaPhytopath. Z.1087179Google Scholar
  11. Daayf, F., Schmitt, A., Bélanger, R. 1997Evidence of phytoalexins in cucumber leaves infected with powdery mildew following treatment with leaf extracts of Reynoutria sachalinensisPlant Physiol.113719727Google Scholar
  12. Askary, H., Benhamou, N., Brodeur, J. 1997Ultrastructural and cytochemical investigations of the antagonistic effect of Verticillium lecanii on cucumber powdery mildewPhytopathology.87359368Google Scholar
  13. Hoch HC., Prowidenti R. Mycoparasitic relationships 5., Cytology of the Sphaerotheca fuliginea - Tilletiopsis sp. interaction, (Cause of powdery mildew of cucumber, biological control), ill. 15 ref. Phytopathology (USA) 1979; v.69 (4): 359-362.Google Scholar
  14. Béngo, RR., Deacon, JW. 1996Interaction specificity of the biocontrol agent Sporothrix flacculosa: a video microscopy studyPhytopathology.8613171323Google Scholar
  15. Elad, Y., Kirshner, B., Sztejnberg, A. 1998Management of powdery mildew and gray mold of cucumber by Trichoderna harzianum T39 and Ampelomyces quisqualis AQ10Biocontrol.43241251Google Scholar
  16. Rotem, Y., Yarden, O., Sztejnberg, A. 1999The mycoparasite Ampelomyces quisqualis expresses exgA encoding and exo-beta-1631638Google Scholar
  17. Verhaar, MA., Hijwegen, T., Zadoks, JC. 1996Glasshouse experiments on biocontrol of cucumber powdery mildew (Sphaerotheca fuliginea) by mycoparasites Verticillium lecanii and Sporothrix rugulosaBiol Control.6353360Google Scholar
  18. Askary, H., Carrié, Y., Bélanger, R.R., Brodeur, J. 1998Pathogenicity of the fungus Verticillium lecanii to aphids and powdery mildewBiocontrol Science and technology.82332Google Scholar
  19. Verhaar, MA., Hijwegen, T. 1997Efficient production of phialoconidia of Verticillium lecanii for biocontrol of cucumber powdery mildew, Sphaerotheca fuligineaNeth. J. Pl. Path.99101103Google Scholar
  20. Lebeda, A. 1984Screening of wild Cucumis species for resistance cucumber powdery mildew (Erysiphe cichoracearum and Sphaerotheca fuliginea)Sci. Hort.24241249Google Scholar
  21. Celio, GJ., Hausbeck, MK. 1998Conidial germination, infection structure formation, and early colony development of powdery mildew on PoisettiaPhytopathology.88105113Google Scholar
  22. Landa, Z., Osborne, L., Lopez, F., Eyal, J. 1994A bioassay for determining pathogenicity of entomopathogenous fungi on whitefliesReferences Biological Control - theory and applications in pest management.4341350Google Scholar
  23. Vidal, C., Lacey, LA., Fargues, J. 1997Intraspecific variability of Paecilomyces fumosoroseus; effect of temperature on vegetative growthJ Invertebr Pathol.701826Google Scholar
  24. Vidal, C., Fergues, J., Lacey, LA., Jackson, MA. 1998Effect of various liquid culture media on morphology, growth, propagule production, and pathogenic activity to Bemisia argentifolii of the entomopathogenic Hyphomycete, Paecilomyces fumosoroseusMycopathologia.1433346Google Scholar
  25. Wurms, K., Labbé, C., Benhamou, A., B langer, RR. 1999Effects of Milsana and Benzothiazole on the ultrastructure of powdery mildew haustoria on cucumberPhytopathology.89728736Google Scholar
  26. Gomez and Gomez, 1984Google Scholar
  27. Yang, 1995.Google Scholar
  28. Newton et al. 1997.Google Scholar
  29. Landa, Z., Bohatá, A. 1999Compatibility of entomogenous fungus Paecilomyces fumosoroseus with natural insecticides based on azadirachtin and Neem OilCollection of Scientific Papers, Faculty of Agriculture in Ceske Budejovice, Series for Crop Science.1699106Google Scholar
  30. Barnett, HL., Binder, FL. 1973The fungal host-parasite relationshipAnnu Rev Phytopathol.11273292Google Scholar
  31. Lebeda A. (1986). Methods of testing vegetable crops for resistance to plant pathogens 1986; 1: 87-90.Google Scholar
  32. Gams W. CBS Course of mycology 3rd edition. Centraal Bureau voor Schimmel Cultures, Baarn, Institute of the Royal Netherlands Academy of Arts and Science 1987; p. 120.Google Scholar
  33. Sundheim, L. 1982Control of cucumber powdery mildew by the hyperperasite Ampelomyces quisqualis and fungicidesPlant Pathol.31209214Google Scholar
  34. Urquhart, JE, Menzies, JG., Punja, ZK. 1994Growth and biological control activity of Tilletiopsis species against powdery mildew (Sphaerotheca fuliginea) on greenhouse cucumberPhytopathology.84341351Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Faculty of Biology, Dept. of BotonyUniversity of South BohemiaČeské BudějoviceCzechia
  2. 2.Dept. of Genetic, Faculty of AgricultureUniversity of South BohemiaČeské BudějoviceCzech Republic

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