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Decreased mRNA expression of NR1H3 and ABCA1 in pulmonary tuberculosis patients from population of Punjab, India

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Abstract

Background

Mycobacterium tuberculosis (MTB) is the causative organism of tuberculosis. Cholesterol is a crucial carbon source required for the survival of MTB in host cells. Transcription factor NR1H3 along with its important target genes ABCA1 and ApoE play important role in removal of extra cholesterol from cells. Changes in the gene expression of NR1H3, ABCA1 and ApoE can affect cholesterol homeostasis and thus the survival of MTB in host cells.Therefore, the present study was designed to analyze the mRNA expression of NR1H3, ABCA1 and ApoE in pulmonary TB (PTB) patients from the population of Punjab, India.

Methods and results

In this study, mRNA expression of the transcription factor NR1H3 and its target genes ABCA1 and ApoE was analyzed in 89 subjects, including 41 PTB patients and 48 healthy controls (HCs) by real-time quantitative PCR. It was found that the mRNA expression of both NR1H3 and ABCA1 genes was significantly lower in TB patients than in HCs (p < 0.001). Even after sex-wise stratification of the subjects, mRNA expression of NR1H3 and ABCA1 was found to be down-regulated in both male and female TB patients. No significant difference was observed in expression of ApoE (p = 0.98).

Conclusions

The present study found that the mRNA expression of NR1H3 and ABCA1 is down-regulated in TB patients from Punjab state of India.

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Data availability

Data are provided within the manuscript or supplementary information files.

Abbreviations

NR1H3 :

Nuclear receptor subfamily 1 group H member 3

ABCA1 :

ATP binding cassette transporter A1

PPARγ :

Peroxisome proliferator activated receptor γ

TB:

Tuberculosis

PTB:

Pulmonary tuberculosis

MTB:

Mycobacterium tuberculosis

NR:

Nuclear receptor

TF:

Transcription factor

RXR:

Retinoic acid receptor

ApoE :

Apolipoprotein E

A poA-1 :

Apolipoprotein-A1

RCT:

Reverse cholesterol transport

HDL:

High density lipoprotein

HCs:

Healthy controls

ATT:

Anti-tuberculosis therapy

GAPDH :

Glyceraldehyde-3-phosphate dehydrogenase

mRNA:

Messenger ribonucleic acid

RXR:

Retinoid X receptor

EDTA:

Ethylenediamine tetraacetic acid

cDNA:

Complementary deoxyribonucleic acid

RT-PCR:

Real-time polymerase chain reaction

GOI:

Gene of interest

CT:

Cycle threshold

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Acknowledgements

The authors are thankful to all the participants enrolled in this study. Anju Kumari is thankful to the Council of Scientific and Industrial Research (CSIR), New Delhi for a junior research fellowship (JRF) and a senior research fellowship (SRF). The authors are also thankful to District TB Officer, Hoshiarpur, Punjab for their help in blood sample collection.

Funding

No funding was received for conducting this study.

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Authors and Affiliations

  1. Department of Zoology, Panjab University, Sector-14, Chandigarh, 160014, India

    Anju Kumari & Vijay Kumar

  2. Department of Pulmonary Medicine, Government Medical College and Hospital, Sector-32, Chandigarh, India

    Varinder Saini

Authors
  1. Anju Kumari
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  2. Varinder Saini
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  3. Vijay Kumar
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Contributions

Conceptualization: [Anju Kumari, Vijay Kumar]; Methodology: [Anju Kumari, Vijay Kumar]; Formal analysis and Investigation: [Anju Kumari, Vijay Kumar]; Resources: [Anju Kumari, Vijay Kumar, Varinder Saini]; Writing-original draft: [Anju Kumari]; Writing-review & editing: [Vijay Kumar]; Supervision: [Vijay Kumar, Varinder Saini]; Validation and Data curation: [Vijay Kumar, Varinder Saini]. All authors have read and approved the manuscript.

Corresponding author

Correspondence to Vijay Kumar.

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Competing interests

The authors declare no competing interests.

Ethical approval

The study was approved by Institutional Ethics Committees of Government Medical College and Hospital, Sector-32, Chandigarh (GMCH/IEC/2020/320/115) and Panjab University, Chandigarh (PUIEC/EC-D-2103-33).

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Informed consent was obtained from all individual participants included in the study.

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Kumari, A., Saini, V. & Kumar, V. Decreased mRNA expression of NR1H3 and ABCA1 in pulmonary tuberculosis patients from population of Punjab, India. Mol Biol Rep 51, 657 (2024). https://doi.org/10.1007/s11033-024-09589-0

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