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The ameliorative effect of vinpocetine against gentamicin-induced uterine-injury in rats involves the inflammasome/caspase-1/IL-1β pathway

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Abstract

Background

There is limited data regarding the hazardous effect of gentamicin (GM) on the uterus and whether or not vinpocetine (Vinpo) ameliorates it. The present study aimed to identify the possible protective effect of Vinpo in GM-induced uterine injury in rats.

Methods

Female rats were assorted in control-group, Vinpo-group, GM-group, and Vinpo plus GM group. Serum and uterine GM concentration were measured. Uterine oxidative stress parameters besides inflammatory and apoptotic biomarkers were evaluated. Uterine histopathological examination and interlukin-1beta (IL-1β) immune-histochemical study were detected.

Results

GM significantly increased uterine oxidative stress, inflammatory and apoptotic biomarkers. Histopathological picture of uterine damage and increased IL-1β immunoexpression were detected. Vinpo significantly ameliorated the distributed GM concentration, oxidative stress, inflammatory and apoptotic biomarkers with a prompt improvement in histopathological picture and a decrease in IL-1β immunoexpression.

Conclusion

Vinpo protective effect against GM-induced uterine injury involves modulation of inflammasome/caspase-1/IL-1β signaling pathway.

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Data availability

All data are available in Prism files as supplementary material on request.

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Funding

The author(s) received no financial support for the research, authorship, and/or publication of this article.

Author information

Authors and Affiliations

  1. Department of Medical Pharmacology, Faculty of Medicine, Minia University, Minia, 61511, Egypt

    Ayman Geddawy & Walaa Yehia Abdelzaher

  2. Department of Basic Medical Sciences, College of Medicine, Prince Sattam Bin Abdulaziz University, 11942, Al Kharj, Saudi Arabia

    Ayman Geddawy

  3. Department of Pathology, Faculty of Medicine, Minia University, Minia, 61511, Egypt

    Mina Ezzat Attya

  4. Department of Obstetrics & Gynecology, Faculty of Medicine, Minia University, Minia, 61511, Egypt

    AbdelRahman Hegazy & Mostafa Kamal AbdElhaseeb

  5. Department of Biochemistry, Faculty of Pharmacy, Minia University, Minia, 61511, Egypt

    Michael Atef Fawzy

  6. Department of Anatomy, Faculty of Medicine, Minia University, Minia, 61511, Egypt

    Medhat Atta

  7. Medical Physiology Department, Faculty of Medicine, Minia University, Minia, 61511, Egypt

    Fatma F. Ali

  8. Biochemistry, Molecular biology and Physiology Department, Faculty of Medicine, Mutah University, Mutah, Jordan

    Fatma F. Ali

Authors
  1. Ayman Geddawy
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  8. Walaa Yehia Abdelzaher
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Contributions

AG and WYA contributed in selecting the point of research, data analysis, drafting, revising the article, give final approval of the version to be published, and agree to be accountable for all aspects of the work. MEA performed and wrote the part of histopathology, and immunohistochemistry. MA participated in data analysis and methodology. MAF performed Western blotting measurements. AH, MKA and FFA participated in writing and editing the manuscript. All authors revised the final copy of the manuscript.

Corresponding author

Correspondence to Walaa Yehia Abdelzaher.

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All authors declare they have no conflict of interest.

Ethics statement

The handling and treatment of rats adhered to the guidelines outlined by the Institutional Ethical Committee (Faculty of Medicine, Minia University, Egypt) of experimental animals and also followed the National Institutes of Health’s (NIH) Guide for the care and utilization of laboratory animals (Approval No: 397:2022; the approval date: 13/12/2022), Faculty of Medicine, Minia University, Egypt.

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Geddawy, A., Attya, M.E., Hegazy, A. et al. The ameliorative effect of vinpocetine against gentamicin-induced uterine-injury in rats involves the inflammasome/caspase-1/IL-1β pathway. Mol Biol Rep 51, 655 (2024). https://doi.org/10.1007/s11033-024-09556-9

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