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RIPK3 and RIPK1 gene expression in pterygium: unveiling molecular insights into pathogenesis

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Abstract

Background

Pterygium, characterized by the abnormal proliferation of epithelial cells, matrix remodeling, vascularization, and lesion migration, is a prevalent ocular surface disease involving the growth of fibrovascular tissue on the cornea. Despite the unclear underlying causes of pterygium, numerous investigations have indicated the involvement of cell death pathways in the regulation of cell cycle dynamics. Consequently, the objective of this study was to assess the expression levels of necroptosis markers in individuals diagnosed with pterygium, aiming to shed light on the potential role of necroptosis in the pathogenesis of this condition.

Methods

This study aimed to investigate the expression patterns of receptor-interacting serine/threonine kinase 3 (RIPK3) and receptor-interacting serine/threonine kinase 1 (RIPK1) genes in pterygium tissues. 41 patients undergoing pterygium excision surgery were recruited. Resected pterygium samples and normal conjunctival tissues were collected, and RIPK3 and RIPK1 mRNA levels were measured using quantitative real-time PCR.

Results

Our findings reveal that the expression of RIPK3 is significantly increased in samples obtained from individuals with pterygium. However, no significant alterations were observed in the expression of RIPK1 in these samples. Results showed significantly higher RIPK3 expression in pterygium tissues compared to controls. Moreover, increased RIPK3 levels correlated negatively with pterygium recurrence rates.

Conclusions

These findings suggest RIPK3 may play a protective role against pterygium recurrence through necroptosis.

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Data availability

Data available on request.

References

  1. Shahraki T, Arabi A, Feizi S (2021) Pterygium: an update on pathophysiology, clinical features, and management. Therapeutic Advances in Ophthalmology, p 13

  2. Nemet AY, Vinker S, Segal O, Mimouni M, Kaiserman I (2016) Epidemiology and associated morbidity of pterygium: a large, community-based casecontrol study. Semin Ophthalmol 31(5):446–451

    PubMed  Google Scholar 

  3. Chu WK, Choi HL, Bhat AK et al (2020) Pterygium: new insights. Eye 34:1047–1050

    Article  PubMed  PubMed Central  Google Scholar 

  4. Donald TAN (2000) Apoptosis and apoptosis related gene expression in normal conjunctiva and pterygium. Br J Ophthalmol 84(2):212–216

    Article  Google Scholar 

  5. Morgan MJ, Kim YS (2022) Roles of RIPK3 in necroptosis, cell signaling, and disease. Exp Mol Med 54(10):1695–1704

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Ofengeim D (2013) Regulation of RIP1 kinase signalling at the crossroads of inflammation and cell death. Nat Rev Mol Cell Biol 14:727–736

    Article  CAS  PubMed  Google Scholar 

  7. Yuping LIU et al (2019) RIP1/RIP3-regulated necroptosis as a target for multifaceted disease therapy. Int J Mol Med 443:771–786

    Google Scholar 

  8. Thapa RJ et al (2013) Interferon-induced RIP1/RIP3mediated necrosis requires PKR and is licensed by FADD and caspases. Proc. Natl Acad. Sci. USA 110, E3109–E3118

  9. Jing-Jie PENG et al (2020) Involvement of regulated necrosis in blinding diseases: focus on necroptosis and ferroptosis. Exp Eye Res 191:107922

    Article  Google Scholar 

  10. Ki-Hong JANG et al (2019) Protective effect of RIPK1-inhibitory compound in in vivo models for retinal degenerative disease. Exp Eye Res 180:8–17

    Article  Google Scholar 

  11. GAO, Sheng et al (2021) Investigation on the expression regulation of RIPK1/RIPK3 in the retinal ganglion cells (RGCs) cultured in high glucose. Bioengineered 12(1):3947–3956

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. SCARPELLINI, Camilla et al (2023) The potential role of regulated cell death in dry eye diseases and ocular surface dysfunction. Int J Mol Sci 24(1):731

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. GUO, Xiao-Xiao, et al. The role of regulated necrosis in inflammation and ocular surface diseases. Exp Eye Res, (2023) 109537

  14. VAN ACKER, Sara I et al (2021) Pterygium—the good, the bad, and the ugly. Cells 10(7):1567

    Article  Google Scholar 

  15. Shanhui LIU et al (2021) RIPK3 signaling and its role in the pathogenesis of cancers. Cell Mol Life Sci, 1–19

  16. Qing-Yang FENG et al (2017) Aberrant expression of genes and proteins in pterygium and their implications in the pathogenesis. Int J Ophthalmol 10(6):973

    Google Scholar 

  17. Samin HONG et al (2008) Expression of neurotrophic factors in human primary pterygeal tissue and selective TNF-α-induced stimulation of ciliary neurotrophic factor in pterygeal fibroblasts. Exp Toxicol Pathol 60(6):513–520

    Article  Google Scholar 

  18. Wang L, Du F, Wang X (2008) TNF-α induces two distinct caspase-8 activation pathways. Cell 133:693–703

    Article  CAS  PubMed  Google Scholar 

  19. LIANG, Kun et al (2011) Expression of cell proliferation and apoptosis biomarkers in pterygia and normal conjunctiva. Mol Vis 17:1687

    CAS  PubMed  PubMed Central  Google Scholar 

  20. Eduardo CARDENAS-CANTU et al (2016) Molecular basis of pterygium development. Seminars in ophthalmology. Informa Healthcare, pp 567–583

  21. Suarez MF, Echenique J, López JM, Medina E, Irós M, Serra HM, Fini ME (2021) Transcriptome Analysis of Pterygium and Pinguecula Reveals Evidence of Genomic Instability Associated with chronic inflammation. Int J Mol Sci 22(21):12090. https://doi.org/10.3390/ijms222112090PMID: 34769520; PMCID: PMC8584501

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. de Guimarães JA, Hounpke BW, Duarte B, Boso ALM, Viturino MGM, de Carvalho Baptista L, de Melo MB, Alves M (2022) Transcriptomics and network analysis highlight potential pathways in the pathogenesis of pterygium. Sci Rep 12(1):286. https://doi.org/10.1038/s41598-021-04248-xPMID: 34997134; PMCID: PMC8741985

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Funding

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Authors and Affiliations

  1. Department of Biology, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran

    Mahnaz Divandari, Arezoo Baradaran moghadam & Ali Akbar Janatabadi

  2. Immunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of medical sciences, Mashhad, Iran

    Amin Javadifar

Authors
  1. Mahnaz Divandari
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  2. Amin Javadifar
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  3. Arezoo Baradaran moghadam
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  4. Ali Akbar Janatabadi
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Contributions

A.J and AA.J wrote the main manuscript text and M.D and A.B prepared figure and tables.

Corresponding author

Correspondence to Ali Akbar Janatabadi.

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Ethical approval

The study protocol was approved by Islamic Azad University of Sabzevar Ethics Committee (IR.IAU.S.REC.1402.005) and obtaining informed consent to participate and consent to publish from the patients in accordance with the guidelines outlined in the Helsinki Declaration (WMA).

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The authors declare no competing interests.

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Divandari, M., Javadifar, A., moghadam, A.B. et al. RIPK3 and RIPK1 gene expression in pterygium: unveiling molecular insights into pathogenesis. Mol Biol Rep 51, 524 (2024). https://doi.org/10.1007/s11033-024-09368-x

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  • DOI: https://doi.org/10.1007/s11033-024-09368-x

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