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Mesenchymal stem cell-derived exosomes decrease Hyperplasia in Psoriasis by inducing transforming growth factor β2 (TGF-β2)

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Abstract

Background

Psoriasis, a chronic inflammatory skin disease, is increasingly effectively managed with the targeted immunotherapy; however, long-term immunotherapy carries health risks, and loss of response. Therefore, we need to develop the alternative treatment strategies. Mesenchymal stem/stromal cell (M.S.C.) exosomes stand out for their remarkable immunomodulatory properties, gaining widespread recognition. This study investigated whether M.S.C. exosomes can reduce psoriasis-induced hyperplasia by inducing Transforming Growth Factor beta 2 (TGF-beta2) signaling.

Methodology

Exosomes were isolated from M.S.C.s by ultracentrifugation. Then, scanning electron microscopy was used for the morphology of exosomes. To ascertain the exosome concentration, the Bradford test was used. To ascertain the cellular toxicity of exosomes in Human Umbilical Vein Endothelial Cells ( H.U.V.E.C), an MTT experiment was then conducted. Real-time PCR was used to quantify TGF beta2 expression levels, whereas an ELISA immunosorbent assay was used to determine the protein concentration of TGF beta2.

Results

In this study, the exosomes of 15–30 nm in size that were uniform, and cup-shaped were isolated. Moreover, the IC50 value for this Treatment was calculated to be 181.750 µg/ml. The concentration of TGF-β2 gene in the target cells significantly increased following Treatment with the exosomes. Furthermore, the expression level of the studied gene significantly increased due to the Treatment.

Conclusion

Upregulating the expression of TGF-β2 in psoriatic cells via TGF-β2 signaling is one way exosomes can help reduce hyperplasia.

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Data availability

No datasets were generated or analysed during the current study.

Abbreviations

MSCs:

. Mesenchymal stem/stromal cells

TGF-β2:

Transforming growth factor-beta 2

HUVEC:

Human umbilical vein endothelial cells

MTX:

Methotrexate

IL-23:

Interleukin 23

TNF-α:

Tumor necrosis factor alpha

A.D:

Atopic Dermatitis

GVHD:

Graft-versus-host disease

FBS:

Fetal bovine serum

PBS:

Phosphate buffered saline

SEM:

Scanning electron microscope

BSA:

Bovine serum albumin

cDNA:

complementary DNA

MVBs:

Multivesicular bodies

DCs:

Dendritic cells

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran

    Zahraa Ibrahim Abed

  2. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Mona Arianejad & Zahra Azizi

Authors
  1. Zahraa Ibrahim Abed
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  2. Mona Arianejad
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  3. Zahra Azizi
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Contributions

Z.I.A. carried out the experiment. M.A. verified the analytical methods and wrote the manuscript and Z.A. supervise the project.

Corresponding author

Correspondence to Zahra Azizi.

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Abed, Z.I., Arianejad, M. & Azizi, Z. Mesenchymal stem cell-derived exosomes decrease Hyperplasia in Psoriasis by inducing transforming growth factor β2 (TGF-β2). Mol Biol Rep 51, 635 (2024). https://doi.org/10.1007/s11033-024-09337-4

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