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Delta of Exopalaemon carinicauda: molecular characterization, expression in different tissues and developmental stages, and its SNPs association analysis with development

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Abstract

Background

The Notch signaling pathway plays a significant role in the gene regulatory network of development of vertebrate and invertebrate. However, as a ligand for the Notch signaling pathway, the mechanism of Delta in the development of Exopalaemon carinicauda is still unclear.

Methods and results

The Delta’s molecular characteristics, tissue distribution and their association with development in E. carinicauda were studied by RACE (rapid amplification of cDNA end), qRT-PCR (quantitative Real-time PCR) and SNP (single nucleotide polymorphism), respectively. The delta in E. carinicauda had a full-length cDNA of 2807 bp and its Delta of 808 amino-acid residue had the highest identity with the Delta of Homarus americanus (identity = 76.63%). Delta had the highest expression in the ovary, and its expression varied with different stages of embryonic, larval, and ovarian development. After delta RNA interference (with a highest interference efficiency of 66% at 24 h), the expression of Notch signaling pathway genes and developmental related genes was significantly reduced, and the ovarian development was significantly delayed. Further study found that there were 4 SNPs (ds1-4) in delta cDNA, of which two (ds2 T1521G caused a mutation Asn422Lys and ds3 G1674A caused a mutation Tyr473Cys in the EGF-like domain) were associated with the development of E. carinicauda. The Gonadosomatic Index (GSI) of the ds2 TT genotypes was 37.28% and 134.60% higher than E. carinicauda of GT and GG genotype respectively (P < 0.05).

Conclusion

Our research indicated that delta was involved in the development of E. carinicauda and provided new insights for molecular breeding with SNP markers in E. carinicauda.

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Data availability

The data generated in this study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, and The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm for their support in experiment instruments and funds.

Funding

This work was supported by Project of Seed-industry Revitalization from the Department of Agriculture and Rural Affairs of Jiangsu Province (JBGS(2021)124), Industry-University-Research Collaboration Fund (JOUH23147), Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Marine Bioresources and Environment, Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province, School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang, 222005, Jiangsu, China

    Xiaofang Lai, Fanyue Ji, Feifan Yu, Hao Chen, Shanrui Shen & Huan Gao

  2. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, 222005, Jiangsu, China

    Xiaofang Lai & Huan Gao

  3. Marine Resource Development institute of Jiangsu (Lianyungang), Lianyungang, 222005, Jiangsu, China

    Xiaofang Lai & Huan Gao

  4. The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, 210014, Jiangsu, China

    Xiaofang Lai & Huan Gao

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  1. Xiaofang Lai
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  2. Fanyue Ji
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  3. Feifan Yu
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  4. Hao Chen
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by XL, SS, FJ, FY, HC and HG. The first draft of the manuscript was written by XL and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Xiaofang Lai.

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Lai, X., Ji, F., Yu, F. et al. Delta of Exopalaemon carinicauda: molecular characterization, expression in different tissues and developmental stages, and its SNPs association analysis with development. Mol Biol Rep 50, 10083–10095 (2023). https://doi.org/10.1007/s11033-023-08840-4

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