Skip to main content

Advertisement

SpringerLink
Log in

Exploring the role of Sirtuin 3 gene polymorphisms and oxidative stress markers in the susceptibility to coronary artery disease

  • Original Article
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Objective

Coronary artery disease (CAD) is a complex disorder influenced by genetic and environmental factors. This case-control study investigated the association between Sirtuin SIRT3 gene polymorphisms, serum malondialdehyde (MDA) levels, and CAD susceptibility.

Methods

Blood samples were collected from 70 CAD cases and 30 controls at the Cardiac Center, Azadi Teaching Hospital, Duhok, Iraq. Genomic DNA was extracted, and PCR-based allele genotyping determined SIRT3 rs11246029 T/C polymorphisms. Serum MDA levels were measured using ELISA. Statistical analysis included t-tests, Mann-Whitney tests, and Spearman correlations. Odds ratios (OR) with 95% confidence intervals (CI) assessed genotypes/alleles and CAD associations. The accuracy of serum MDA in predicting the severity of CAD was evaluated using receiver operating characteristic (ROC) curve analysis.

Results

There were no significant variations in serum MDA levels between controls and CAD patients in the study. The diagnostic accuracy of serum MDA for CAD severity prediction was modest (Area Under Curve (AUC) = 0.56). Correlations revealed associations between MDA and total bilirubin (negative) and Troponin (positive). CRP correlated positively with LDH, glucose, cholesterol, LDL, CKmB, and Troponin. CKmB and Troponin are positively associated with clinical characteristics. Genotype analysis identified a significantly higher CAD risk with the CC genotype compared to controls.

Conclusion

These findings shed light on the potential role of SIRT3 gene polymorphisms and serum MDA levels in CAD susceptibility. Further research is needed to understand underlying mechanisms and therapeutic implications based on these markers.

Trial registration

15092021-9-12. Registered 15 September 2021.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Data Availability

Data will be made available on request.

References

  1. Higashi Y, Gautam S, Delafontaine P, Sukhanov S (2019) IGF-1 and cardiovascular disease. Growth Horm IGF Res 45:6–16

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Ponikowski P, Anker SD, AlHabib KF et al (2014) Heart failure: preventing disease and death worldwide. ESC Heart Failure 1(1):4–25

    Article  PubMed  Google Scholar 

  3. Malakar AK, Choudhury D, Halder B, Paul P, Uddin A, Chakraborty S (2019) A review on coronary artery disease, its risk factors, and therapeutics. J Cell Physiol 234(10):16812–16823

    Article  CAS  PubMed  Google Scholar 

  4. Chen Z, Schunkert H (2021) Genetics of coronary artery disease in the post-GWAS era. J Intern Med 290(5):980–992

    Article  PubMed  Google Scholar 

  5. Merino DM, Ma DW, Mutch DM (2010) Genetic variation in lipid desaturases and its impact on the development of human disease. Lipids Health Dis 9:1–14

    Article  Google Scholar 

  6. Shen Y, Wu Q, Shi J, Zhou S (2020) Regulation of SIRT3 on mitochondrial functions and oxidative stress in Parkinson’s disease. Biomed Pharmacother 132:110928

    Article  CAS  PubMed  Google Scholar 

  7. Halim M, Halim A (2019) ;13(2):1165-72

  8. Ikonomidis I, Makavos G, Papadavid E et al (2015) Similarities in coronary function and myocardial deformation between psoriasis and coronary artery disease: the role of oxidative stress and inflammation. Can J Cardiol 31(3):287–295

    Article  PubMed  Google Scholar 

  9. Zhai X-W, Zhang Y-L, Qi Q et al (2013) Effects of molybdenum on sperm quality and testis oxidative stress. Syst Biol Reprod Med 59(5):251–255

    Article  CAS  PubMed  Google Scholar 

  10. Ho E, Karimi Galougahi K, Liu C-C, Bhindi R, Figtree GA (2013) Biological markers of oxidative stress: applications to cardiovascular research and practice. Redox Biol 1(1):483–491

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Adam LN, Oraha AY, Shekha MS, Al-Habib OAM (2023) Exploring nitric oxide as a crucial prognostic biomarker of coronary artery disease. Prostaglandins Other Lipid Mediat 165:106717

    Article  CAS  PubMed  Google Scholar 

  12. Noshin TF, Ali MR, Banik S (2021) Increased oxidative stress and altered serum macro-minerals and trace elements levels are associated with coronary artery disease. J Trace Elem Med Biol 64:126707

    Article  CAS  PubMed  Google Scholar 

  13. Yaghoubi A, Ghojazadeh M, Abolhasani S, Alikhah H, Khaki-Khatibi F (2015) Correlation of serum levels of vitronectin, malondialdehyde and Hs- CRP with Disease Severity in Coronary Artery Disease. J Cardiovasc Thorac Res 7(3):113–117

    Article  PubMed  PubMed Central  Google Scholar 

  14. Kotani K, Tashiro J, Yamazaki K et al (2015) Investigation of MDA-LDL (malondialdehyde-modified low-density lipoprotein) as a prognostic marker for coronary artery disease in patients with type 2 diabetes mellitus. Clin Chim Acta 450:145–150

    Article  CAS  PubMed  Google Scholar 

  15. Aladağ N, Asoğlu R, Ozdemir M et al (2021) Oxidants and antioxidants in myocardial infarction (MI): investigation of ischemia modified albumin, malondialdehyde, superoxide dismutase and catalase in individuals diagnosed with ST elevated myocardial infarction (STEMI) and non-STEMI (NSTEMI). J Med Biochem 40(3):286–294

    Article  PubMed  PubMed Central  Google Scholar 

  16. Lee B-J, Huang Y-C, Chen S-J, Lin P-T (2012) Coenzyme Q10 supplementation reduces oxidative stress and increases antioxidant enzyme activity in patients with coronary artery disease. Nutrition 28(3):250–255

    Article  CAS  PubMed  Google Scholar 

  17. Palazhy S, Kamath P, Vasudevan DM (2015) Elevated oxidative stress among coronary artery disease patients on statin therapy: a cross sectional study. Indian Heart J 67(3):227–232

    Article  PubMed  PubMed Central  Google Scholar 

  18. Grosser N, Abate A, Oberle S et al (2003) Heme oxygenase-1 induction may explain the antioxidant profile of aspirin. Biochem Biophys Res Commun 308(4):956–960

    Article  CAS  PubMed  Google Scholar 

  19. Undas A, Brummel-Ziedins K, Mann KG (2014) Why does aspirin decrease the risk of venous thromboembolism? On old and novel antithrombotic effects of acetyl salicylic acid. J Thromb Haemost 12(11):1776–1787

    Article  CAS  PubMed  Google Scholar 

  20. Falco L, Tessitore V, Ciccarelli G et al (2023) Antioxidant Properties of oral antithrombotic therapies in atherosclerotic Disease and Atrial Fibrillation. Antioxidants 12(6):1185

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Aseri Z, Habib SS, Alhomida AS, Khan HA (2014) Relationship of high sensitivity C-reactive protein with cardiac biomarkers in patients presenting with acute coronary syndrome. J Coll Physicians Surg Pak 24(24):387–391

    PubMed  Google Scholar 

  22. Kojima S, Funahashi T, Maruyoshi H et al (2005) Levels of the adipocyte-derived plasma protein, adiponectin, have a close relationship with atheroma. Thromb Res 115(6):483–490

    Article  CAS  PubMed  Google Scholar 

  23. Nissen SE, Tuzcu EM, Schoenhagen P et al (2005) Statin therapy, LDL cholesterol, C-Reactive protein, and coronary artery disease. N Engl J Med 352(1):29–38

    Article  CAS  PubMed  Google Scholar 

  24. Wu J, Zeng Z, Zhang W et al (2019) Emerging role of SIRT3 in mitochondrial dysfunction and cardiovascular diseases. Free Radic Res 53(2):139–149

    Article  CAS  PubMed  Google Scholar 

  25. Song X, Wang H, Wang C et al (2022) Association of Sirtuin Gene polymorphisms with susceptibility to coronary artery disease in a North Chinese Population. Biomed Res Int 2022:4294008

    Article  PubMed  PubMed Central  Google Scholar 

  26. Altamemi IA-M, Hassan AR, Jawad A (2018) Impact of SIRT3 gene polymorphism on Acute Myocardial Infarction susceptibility. Res J Pharm Biol Chem Sci 9(6):1757

    Google Scholar 

  27. Habieb MSE-D, Abdel-Aziz WF, Ismail AHA, Sallam KMA, El-Shafie MK (2021) Sirtuin1 and Sirtuin3 gene polymorphisms and acute myocardial infarction susceptibility. Meta Gene 30:100965

    Article  CAS  Google Scholar 

  28. Yin X, Pang S, Huang J, Cui Y, Yan B (2016) Genetic and functional sequence variants of the SIRT3 gene promoter in myocardial infarction. PLoS ONE 11(4):e0153815

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The authors express their gratitude to the Cardiac Center/Azadi Teaching Hospital team for their continuous support during the sample collection process. Special thanks are extended to Dr Ashur Y. Oraha, a cardiovascular surgeon, who played a crucial role in patient selection. Additionally, the authors would like to acknowledge the assistance provided by Mr. Govand in conducting the genetic study.

Funding

This research received no specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Zakho, Duhok, Kurdistan Region, Iraq

    Lina N. Adam

  2. Department of Biology, College of Science, University of Nawroz, Duhok, Kurdistan Region, Iraq

    Omar A. M. Al-Habib

  3. Department of medical cell biology, Upsala University, Upsala, Sweden

    Mudhir S. Shekha

  4. Department of Biology, College of Science, Salahaddin University -Erbil, Erbil, Kurdistan Region, Iraq

    Mudhir S. Shekha

Authors
  1. Lina N. Adam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Omar A. M. Al-Habib
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mudhir S. Shekha
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Lina N. Adam was responsible for collecting the patients’ data, conducting the genetic analysis, interpreting the results, and writing the manuscript. Omar A.M. AL Habib and Mudhir S. Shekha supervised the project and conceived the original idea. All authors contributed to the revision of the manuscript and reviewed and approved the final version.

Corresponding author

Correspondence to Lina N. Adam.

Ethics declarations

Ethical approval

The Research Ethics Committee of the Duhok Directorate General of Health in Duhok, Iraq, accepted the study protocol “Trial registration: 15092021-9-12. Registered 15 September 2021”.

Competing interests

None.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Adam, L.N., Al-Habib, O.A.M. & Shekha, M.S. Exploring the role of Sirtuin 3 gene polymorphisms and oxidative stress markers in the susceptibility to coronary artery disease. Mol Biol Rep 50, 9221–9228 (2023). https://doi.org/10.1007/s11033-023-08825-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-023-08825-3

Keywords

Search

Navigation