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The relationship between DNA methyltransferase 3B (DNMT3B) and miR 124-3pa expressions in bladder cancer tissues

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Abstract

Background

Cancer bladder is the most common malignant tumor affecting the urinary tract. Genetic alterations are tightly associated with the development of cancer bladder. MicroRNAs (miRNA) are small, noncoding single-stranded RNA molecules that have been linked to bladder cancer. miR-124-3pa exhibits altered expression in various types of human malignancies. DNA methyltransferase 3B (DNMT3B) is responsible for de novo DNA methylation which is a fundamental epigenetic process in carcinogenesis. This work was performed to study the expression of DNMT3B and miR 124-3pa in bladder cancer tissues, and investigate their significance in the diagnosis and prognosis of the disease.

Subjects & methods

This case-control study included one hundred and six tissue samples of patients with primary urothelial bladder cancer. The tissues were separated into two parts. The first part was immediately frozen and kept at − 80 °C for total RNA extraction with subsequent detection of miR 124-3pa and DNMT3B expressions. The other part was preserved in formalin solution for histopathological examination.

Results

There was a highly statistically significant difference between the cancerous and the normal tissues as regarding miRNA-124-3pa and DNMT3B expression (P < 0.001) for each. Also, there was a highly statistically significant strong negative correlation between miRNA-124-3pa and DNMT3B expression (r=-0.750, P < 0.001). The combined performance of miR-124-3pa and DNMT3B revealed that the cutoff point of ≥ 3.3 can be used as a predictor of the presence of cancer bladder with sensitivity of 98.1% and specificity of 80%.

Conclusion

miR-124-3pa and DNMT3B can be used as predictors of the presence of cancer bladder.

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Data Availability

Available upon a reasonable request.

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Authors and Affiliations

  1. Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Mohammed S. Fawzy, Ansam M. Z. El Desoky & Samia Hussein

  2. Urology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Abdel Rahman M. El Faiomy

  3. Department of Basic Medical Sciences, Ibn Sina University for Medical Sciences, Amman, Jordan

    Samia Hussein

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  1. Mohammed S. Fawzy
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  2. Abdel Rahman M. El Faiomy
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  3. Ansam M. Z. El Desoky
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  4. Samia Hussein
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Contributions

MSF, ARME, and SH designed and directed the project. SH and AMZE performed the experiments, analyzed the data, and wrote the manuscript which was revised by all co-authors.

Corresponding author

Correspondence to Samia Hussein.

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The authors declare no competing interests.

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The study protocol was approved by the Institute Review Board of the Faculty of Medicine, Zagazig University (ZU-IRB#9014/25-10-2021).

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Fawzy, M.S., El Faiomy, A.R.M., El Desoky, A.M.Z. et al. The relationship between DNA methyltransferase 3B (DNMT3B) and miR 124-3pa expressions in bladder cancer tissues. Mol Biol Rep 50, 10005–10013 (2023). https://doi.org/10.1007/s11033-023-08818-2

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  • DOI: https://doi.org/10.1007/s11033-023-08818-2

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