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Association of a novel begomovirus species with fenugreek yellow vein disease in India

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Abstract

Background

Fenugreek (Trigonella foenum-graecum L.) is an annual medicinal and spice crop belonging to the family Fabaceae. The occurrence of a yellow vein disease was recorded in fenugreek in Jodhpur (India) in 2022. The infection of begomoviruses in legume crops results in significant yield loss and major economic loss. The current study reports an association of a novel begomovirus species associated with yellow vein disease in Fenugreek.

Methods and results

In symptomatic fenugreek plants, geminivirus-like particles were visible under a transmission electron microscope. Further, nucleotide sequence analysis of the rolling circle amplified product revealed 2743 nucleotide DNA-A genome with close relatedness to French bean leaf curl virus (88.21%) and Senna leaf curl virus (87.63%). It was proposed as a new begomovirus species, Fenugreek yellow vein Rajasthan virus. The genome organization suggested the presence of a typical nonanucleotide sequence along with 7 ORFs in DNA-A. A possible recombination event took place in the coat protein (V1) region with Pedilanthus leaf curl virus and Chilli leaf curl virus as major and minor parents. The recombinant virus poses possible threats to several other legume crops. To the best of our knowledge, this is the first report of the association of FeYVRaV with fenugreek yellow vein disease from northwestern India.

Conclusions

In conclusion, the presence of a novel begomovirus species associated with yellow vein disease in fenugreek is alarming and needs further studies on its infectivity to prevent its spread to legume crops.

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Acknowledgements

We gratefully acknowledge the support received from the IARI, New Delhi, CAZRI, Jodhpur, and Department of Biotechnology, Government of India, New Delhi, India.

Funding

DBT (Grant no. BT/PR40767/AGIII/103/12777/2020).

Author information

Authors and Affiliations

  1. Insect Vector Laboratory, Advanced Centre for Plant Virology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Manish Kumar & Amalendu Ghosh

  2. Department of Plant Pathology, College of Agricultural & Environmental Sciences, University of Georgia, Tifton, GA, 31793, USA

    Manish Kumar

  3. ICAR-Central Arid Zone Research Institute, Jodhpur, Rajasthan, 342003, India

    Kuldeep Singh Jadon, Rajesh K. Kakani & Ramesh K. Solanki

  4. Division of Plant Pathology, TEM Facility, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Baljeet Kaur

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  1. Manish Kumar
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Contributions

KSJ, RKK, and RKS performed the field observation and collection of the infected samples. MK and AG conceived the experiments and MK performed the experiments and generated scientific data. BK performed the TEM analysis. MK and AG analyzed the data and drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Amalendu Ghosh or Kuldeep Singh Jadon.

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Kumar, M., Ghosh, A., Jadon, K.S. et al. Association of a novel begomovirus species with fenugreek yellow vein disease in India. Mol Biol Rep 50, 9203–9211 (2023). https://doi.org/10.1007/s11033-023-08806-6

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