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New report of endophytic bacterium Achromobacter xylosoxidans from root tissue of Musa spp.

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Abstract

Background

Cavendish (AAA) banana plant (Musa spp.) worldwide cultivated crop harbors many endophytic bacteria. Endophytic bacteria are those that live inside plant tissues without producing any visible symptoms of infection.

Results

Endophytic bacterium (MRH 11), isolated from root tissue of Musa spp.was identified as Achromobacter xylosoxidans (ON955872) which showed positive effects in IAA production, phosphate solubilization, catalase production. A. xylosoxidans also showed in vitro antagonism against Curvularia lunata causing leaf spot disease of Cavendish (AAA) banana (G-9 variety). The GC-MS analysis of culture filtrate of A. xylosoxidans (ON955872) confirmed this finding. GC-MS analysis was carried by using two solvent etheyl acetate and chloroform and it showed several antifungal compounds. The identification of these bioactive secondary metabolites compounds was based on the peak area, retention time, molecular weight, molecular formula and antimicrobial actions. GC-MS analysis result revealed the presence of major components including Cyclododecane, 1-Octanol, Cetene, Diethyl phthalate. In vivo test to banana plants was carried out in separate field as well as in potted conditions. Appearance of leaf spots after foliar spray of spore of pathogen and reduction in leaf spots after application of bacterial suspension was found.

Conclusion

The present study has highlighted the role of endophytic bacterium as antagonist to the pathogen Curvularia lunata.

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Data availability

No datasets were generated or analyzed during the current study.

Abbreviations

GC–MS:

Gas chromatography–mass spectrometry

IAA:

Indole acetic acid

MEA:

Malt extract agar

PGPEB:

Plant growth promoting endophytic bacteria

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Acknowledgements

Heredity Life sciences Pvt Ltd, Bhubaneswar, Odisha and Edison Life Science Laboratory, Kolkata, West Bengal, India are thankfully acknowledged for the molecular identification of endophytic bacterial strain and fungus isolate. Authors also acknowledged USIC, University of North Bengal for taking pictures of scanning electron microscopy.

Funding

Authors have not availed any funding support for carrying this research work.

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Authors and Affiliations

  1. Department of Botany, Raiganj University, Uttar Dinajpur, Raiganj, West Bengal, 733134, India

    Papan Chowhan, Shambhu Swarnakar & Arka Pratim Chakraborty

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  1. Papan Chowhan
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  2. Shambhu Swarnakar
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  3. Arka Pratim Chakraborty
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Correspondence to Arka Pratim Chakraborty.

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Chowhan, P., Swarnakar, S. & Chakraborty, A.P. New report of endophytic bacterium Achromobacter xylosoxidans from root tissue of Musa spp.. Mol Biol Rep 50, 9179–9190 (2023). https://doi.org/10.1007/s11033-023-08789-4

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