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Genome-wide analysis of the LAZ1 gene family in Gossypium hirsutum

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Abstract

Background

As the world’s leading fiber crop and a major oil-producing crop, cotton fiber yield and fiber quality are affected by environmental stresses, especially heat, drought and salinity. The LAZ1 (Lazarus 1) family genes are responsive to abscisic acid, drought, and salt treatments. Currently, mining and functional analyses of LAZ1 family genes in cotton have not been reported.

Methods and results

In this study, 20 GhLAZ1 genes, designated GhLAZ1-1 - GhLAZ1-20, were identified in the genome of Gossypium hirsutum through the construction of an HMM model, and their molecular properties, chromosomal localization, phylogeny, gene structure, evolutionary selection pressure, promoter cis elements and gene expression under salt stress were analyzed. With the exception of GhLAZ1-17 and GhLAZ1-20, the remaining 18 GhLAZ1 genes were unevenly localized on 13 chromosomes in G. hirsutum; evolutionary analysis showed that these genes could be divided into three subfamilies; and evolutionary selection pressure analysis demonstrated that the GhLAZ1 genes were all under purifying selection. Many elements related to light responses, hormone responses, and abiotic stresses were predicted on the GhLAZ1 family gene promoters, and real-time quantitative PCR results showed that GhLAZ1-2, GhLAZ1-8, and GhLAZ1-18 were upregulated significantly in salt-treated cotton leaves.

Conclusions

Our results suggested that GhLAZ1 genes were involved in the salt tolerance mechanism in G. hirsutum and provided a reference for further exploring the function and molecular mechanism of LAZ1 genes.

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Data Availability

The genome information files and annotation files of G. hirsutum were downloaded from the NCBI database (https://www.ncbi.nlm.nih.gov/).

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Funding

This work was supported by the National Key R&D Program of China (2021YFE0101200), the PSF/CRP/18th Protocol (07), the National Natural Science Foundation of China (52161145104), and Key Research and Development Project of Jiangsu Province, China (Modern Agriculture, BE2022364).

Author information

Author notes

  1. Tingting Deng and Zongjin Pan these authors contributed equally to this work.

Authors and Affiliations

  1. School of Life Sciences, Nantong University, 226019, Nantong, Jiangsu, China

    Tingting Deng, Yingying Tang, Wenxiang Feng, Dequan Li, Kangtai Sun, Yunying Cao & Baohua Wang

  2. Jiangsu Coastal Area Institute of Agricultural Sciences, Jiangsu Collaborative Innovation Center for Modern Crop Production, 224002, Yancheng, Jiangsu, P.R. China

    Zongjin Pan & Wei Wang

  3. Plant Breeding and Genetics Division, Nuclear Institute for Agriculture and Biology, 38000, Faisalabad, Pakistan

    Allah Ditta & Muhammad K.R. Khan

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  1. Tingting Deng
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Contributions

statement BW and YC conceived the idea and designed the experiment. TD, WW, YT, WF, DL, and KS performed the experiment. TD, ZP, and AD analyzed the data. TD wrote the manuscript. BW, YC, and MKRK revised the manuscript. All the authors have read and approved the final draft.

Corresponding authors

Correspondence to Muhammad K.R. Khan, Yunying Cao or Baohua Wang.

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The authors declare no financial or non-financial conflict of interest.

Ethical Standards The experiments conducted in this study comply with the current laws of China.

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Deng, T., Pan, Z., Wang, W. et al. Genome-wide analysis of the LAZ1 gene family in Gossypium hirsutum. Mol Biol Rep 50, 9273–9282 (2023). https://doi.org/10.1007/s11033-023-08788-5

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  • DOI: https://doi.org/10.1007/s11033-023-08788-5

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