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Variability in the PRDM9 gene in Sindhi cattle

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Abstract

Background

Sindhi is a dual-purpose breed adapted to tropical environments. However, this breed has the smallest total population among indicine breeds in Brazil and the smallest effective number. In addition, the inbreeding coefficient is higher than 6.25% in ~ 60% of the population. Therefore, alternatives to increase genetic diversity are important. Within this context, the PRDM9 gene is particularly interesting since it is involved in meiotic recombination events, consequently enhancing genetic variability in the population by increasing the number of circulating haplotypes. Each allele of the gene induces recombination at a different hotspot. The larger the number of circulating alleles, the higher the recombination rate and the greater the genetic variability.

Methods

The aim of this study was to characterize alleles of the PRDM9 gene in Sindhi cattle. The region of the zinc finger domains of the gene was amplified by PCR, genotyped, and sequenced for allele identification in 50 Sindhi animals.

Results

Three alleles (A-cattle1, B-cattle14, and C-cattle19) and six genotypes (AA, BB, CC, AB, AC, and BC) were identified.

Conclusion

The allele variation of the PRDM9 gene in the Sindhi breed enables to guide the mating of animals with different genotypes/alleles and to promote genetic variability by recombination if there is intralocus variability.

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Data Availability

DNA sequence are available at GenBank website under accession number: OR076063-66.

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Acknowledgements

The authors thank the association “Núcleo Nordeste de Criadores de Sindi” for contacting the breeders and organizing the collection of the biological material and authorization of collection. We also acknowledge the National Council for Scientific and Technological Development (CNPq) for the fellowship granted to the first author (grant number 126642/2020-6).

Funding

Not applicable.

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Authors and Affiliations

  1. Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia, (UFBA), Salvador, BA, Brasil

    Victoria Camilla Parente Rocha, Jackeline Santos Alves, Raphael Bermal Costa & Gregório Miguel Ferreira de Camargo

Authors
  1. Victoria Camilla Parente Rocha
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  2. Jackeline Santos Alves
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  3. Raphael Bermal Costa
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  4. Gregório Miguel Ferreira de Camargo
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Contributions

VCPR, JSA, RBC and GMFC analyzed the data, carried out the experiments discussed the results and drafted the manuscript. GMFC conceived the study. VCPR wrote the manuscript. All authors read and approved the final version of the manuscript.

Corresponding author

Correspondence to Gregório Miguel Ferreira de Camargo.

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Ethics approval and consent to participate

The Ethics Committee on Animal Use of EMVZ-UFBA approved the project (80/2018).

Competing interests

The authors declare no conflict of interest.

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Rocha, V.C.P., Alves, J.S., Costa, R.B. et al. Variability in the PRDM9 gene in Sindhi cattle. Mol Biol Rep 50, 8839–8842 (2023). https://doi.org/10.1007/s11033-023-08778-7

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  • DOI: https://doi.org/10.1007/s11033-023-08778-7

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