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Latex C-serum from Hevea brasiliensis induces apoptotic cell death in a leukemic cell line

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Abstract

Background

Hevea brasiliensis latex is generally cultivated for the use of rubber particles. Previous studies have shown that the antiproliferative activity of C-serum in hepatocellular carcinoma is not induced through the classical apoptotic signaling pathway. However, in a leukemic cell line, the anti-proliferation effect of latex C serum remained unclear.

Methods

Leukemic cell lines (K562 and U937) and human peripheral blood mononuclear cells (PBMCs) were examined for cell viability using the MTT assay. Flow cytometry was used for apoptotic cell detection by annexin V/PI staining. The expression levels of proapoptotic and antiapoptotic marker genes were measured by qRT‒PCR. Moreover, the caspase activities of the extrinsic and intrinsic apoptotic pathways were detected by enzymatic activities.

Results

Latex C-serum inhibited cell proliferation in the K562 and U937 leukemic cell lines but did not affect human PBMCs. Latex C-serum significantly induced the percentage of early and late apoptotic cells in the leukemic cell line. The expression levels of the pro-apoptotic marker genes BAD, BAX, and CASPASE3 significantly increased in the leukemic cell line after post-latex C-serum leukemic cell treatment. The extrinsic, intrinsic and common apoptotic pathways were also studied through caspase-8, -9, and -3 activities. Latex C-serum treatment significantly induced caspase-8, -9, and -3 activation in the K562 cell line and U937 cell line compared to the untreated cells.

Conclusions

These results indicate that latex C-serum enhanced anti-proliferation in leukemic cell lines by inducing apoptosis and caspase activation.

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Data availability

No data associated in the manuscript.

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Acknowledgements

This work was supported by the National Science, Research and Innovation Fund (NSRF) and Prince of Songkla University (Grant No. MET6505111S) and the Faculty of Medical Technology, Prince of Songkla University.

Funding

The National Science, Research and Innovation Fund (NSRF) and Prince of Songkla University (Grant No. MET6505111S).

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Authors and Affiliations

  1. Faculty of Medical Technology, Prince of Songkla University, 15, Kanjanavanit Rd. Hat Yai, Songkhla, 90110, Thailand

    Hansuk Buncherd, Sawitree Hongmanee, Charinrat Saechan, Natta Tansila, Supinya Thanapongpichat & Kanitta Srinoun

  2. Medical Science Research and Innovation Institute, Research and Development Office, Prince of Songkla University, Songkhla, Thailand

    Worrawit Wanichsuwan

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  1. Hansuk Buncherd
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Contributions

KS was the principal investigator and had primary responsibility for the conception and design of the project, helping perform the experiments, data analysis, and drafting and editing of the manuscript. HB contributed to the study design, interpretation of the data and editing of the manuscript. SH performed the experiments and analyzed the data. CS performed the preparation of latex C-serum and editing of the manuscript. NT, ST, and WW contributed to the data analysis and editing of the manuscript. All authors reviewed and approved the final version to be submitted for publication.

Corresponding author

Correspondence to Kanitta Srinoun.

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The authors declare no conflicts of interest.

Ethical approval

This study was approved by the Ethics Committee, Faculty of Medical Technology, Prince of Songkla University [approval ID EC65-01].

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Buncherd, H., Hongmanee, S., Saechan, C. et al. Latex C-serum from Hevea brasiliensis induces apoptotic cell death in a leukemic cell line. Mol Biol Rep 50, 7515–7525 (2023). https://doi.org/10.1007/s11033-023-08687-9

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  • DOI: https://doi.org/10.1007/s11033-023-08687-9

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