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Effect of heterologous platelet-rich plasma on liver and modulation of glucose metabolism and Wnt signalling pathways in diabetic mice

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Abstract

Background

The current study was designed to highlight the effects of heterologous platelet-rich plasma (PRP) on deteriorated hepatic tissues and impaired glucose metabolism of alloxan-induced diabetic mice.

Methods

30 male mice were divided into a control (CG), PRP (PG), diabetic (DG), and two treated groups (T1G and T2G). PG was given PRP treatment (0.5 ml/kg body weight) twice a week for four weeks. DG, T1G and T2G were given alloxan (150 mg/kg) to induce diabetes. After confirmation, PRP treatment was given to T1G and T2G for two and four weeks respectively while DG was left untreated. Upon completion of the said experimental period, liver samples were taken for histological and gene expression analyses.

Results

The study found that the liver tissue of the DG group showed signs of damage, including hepatocyte ballooning, sinusoid dilatation, and collagen deposition. However, these changes were significantly reduced in both T1G and T2G groups. The expression of several genes related to liver function was also affected, with upregulation of Fbp1 and Pklr, and downregulation of Pck1 in the DG group. PRP treatment restored Fbp1 expression and also increased the expression of glycolytic pathway genes Hk1 and Gck, as well as Wnt signalling pathway genes Wnt2, Wnt4, and Wnt9a in both treated groups.

Conclusion

Current study revealed that heterologous PRP may partly alleviate high glucose levels in diabetics possibly by mediating glucose metabolism via inhibition of Wnt signalling pathway.

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Abbreviations

DG:

Diabetic group

DM:

Diabetes Mellitus

ECM:

Extracellular matrix

GFs:

Growth factors

HSCs:

Hepatic stellate cells

PRP:

Platelet-rich plasma

T1G:

Treated group 1

T2G:

Treated group 2

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Acknowledgements

The authors are highly thankful to the Vice-Chancellor, University of the Punjab, Lahore, Pakistan for his support for accomplishing this research work. Furthermore, it stated that this manuscript has been submitted to a preprint platform having DOI: https://doi.org/10.21203/rs.3.rs-1910439/v1 and licensed under a Creative Commons Attribution 4.0 International License.

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Author notes

  1. Amin Arif and Adil Farooq have contributed equally to this work.

Authors and Affiliations

  1. Cell and Molecular Biology Lab, Institute of Zoology, University of the Punjab, Lahore, Pakistan

    Amin Arif, Rabia Mehmood, Tayyaba Saleem & Nadeem Sheikh

  2. Department of Zoology, University of Okara, Okara, Pakistan

    Adil Farooq, Muddasir Hassan Abbasi, Humaira Allay Ali & Mehreen Anjum

  3. Applied Molecular Biology and Biomedicine Lab, Department of Zoology, University of Narowal, Narowal, Pakistan

    Muhammad Babar Khawar

  4. Department of Pharmacology, University of Health Sciences, Lahore, Pakistan

    Tasleem Akhtar

  5. Department of Zoology, University of Central Punjab, Lahore, Pakistan

    Rabia Mehmood

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Contributions

AA and AF performed the major part of this research work, did statistical analysis, interpretation of results, and initial draft. MBK revised the figures, improved the initial draft, and helped in lab work. TA, RM and TS, HAA and MA helped in lab work. MHA and NS conceived the idea, supervised the work, revised, modified, and approved the final draft.

Corresponding authors

Correspondence to Muddasir Hassan Abbasi or Nadeem Sheikh.

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Arif, A., Farooq, A., Abbasi, M.H. et al. Effect of heterologous platelet-rich plasma on liver and modulation of glucose metabolism and Wnt signalling pathways in diabetic mice. Mol Biol Rep 50, 7145–7154 (2023). https://doi.org/10.1007/s11033-023-08600-4

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