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Polysaccharide extracted from the Sargassum fusiforme induces cell cycle arrest and apoptosis of B16F10 melanoma cells through the PI3K/AKT pathway

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Abstract

Background. Sargassum fusiforme

(S. fusiforme) is a brown alga that has been utilized as a medicine for a long time. Polysaccharides extracted from S. fusiforme demonstrate antitumor activities.

Methods

The impact of S. fusiforme polysaccharides (SFPS 191,212) on the proliferation, apoptosis, and cell cycle kinetics of B16F10 murine melanoma cells were thoroughly investigated in this work. The anticancer activities of the SFPS 191,212 compounds were assayed in the B16F10 cells at both transcriptional and translational levels.

Results

The compound exhibited concentration-dependent effects. Moreover, SPFS 191,212 increased the numbers of apoptotic cells and arrested the cell cycle in the S phase of the quantitative real-time PCR. From western blotting, it was verified that the SFPS 191,212 treatment improved the expression of Bax, Caspase-9, and Caspase-3 genes and proteins, while it reduced phosphatidylinositol 3 kinase and Bcl-2 genes and proteins, suggesting the involvement of mitochondria.

Conclusion

Overall, SFPS 191,212 can be further explored as a potential functional food or adjuvant agent for the prevention or treatment of melanoma.

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Data Availability

Data will be made available on request.

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Acknowledgements

Not applicable.

Funding

This research was supported by the Ningbo Public Welfare Project (No. 2022S065), the Research Startup Project of the Advanced Talents of the First Affiliated Hospital of Ningbo University (No. 2022KYQDJ-XF), the Medical and Health Research Project of Zhejiang Province (No. 2022KY1145), and the Project of the Ningbo Leading Medical & Health Discipline (No. 2022-F23).

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    Authors and Affiliations

    1. Department of Dermatology, The First Affiliated Hospital of Ningbo University, 247 Renmin Road, Ningbo, 315000, China

      Feng Xu, Zhifang Liu, Xinyu Jiang, Yizhao Ma, Diancheng Wang & Suling Xu

    2. College of Biological and Environmental Sciences, Zhejiang Wanli University, 8 Qianhu South Road, Ningbo, 315100, China

      Haomiao Ding

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    1. Feng Xu
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    Contributions

    Feng Xu, Haomiao Ding, and Suling Xu contributed to the conception of the study. Feng Xu, Haomiao Ding, Zhifang Liu, Xinyu Jiang, Yizhao Ma, and Diancheng Wang performed the experiment. Suling Xu contributed significantly to the analysis and manuscript preparation. Feng Xu performed the data analysis and wrote the manuscript. Haomiao Ding helped perform the analysis with constructive discussion.

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    Correspondence to Suling Xu.

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    Feng Xu and Haomiao Ding contributed equally to this work.

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    Xu, F., Ding, H., Liu, Z. et al. Polysaccharide extracted from the Sargassum fusiforme induces cell cycle arrest and apoptosis of B16F10 melanoma cells through the PI3K/AKT pathway. Mol Biol Rep 50, 6517–6528 (2023). https://doi.org/10.1007/s11033-023-08570-7

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