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A new type of tandem repeats in Myotis petax (Chiroptera, Vespertilionidae) mitochondrial control region

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A Correction to this article was published on 08 September 2023

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Abstract

Background

Tandem repeats in mitochondrial DNA control region are known to different animal taxa, including bat species of the family Vespertilionidae. The long R1-repeats in the bat ETAS-domain are often presented in a variable copy number and may exhibit both inter-individual and intra-individual sequence diversity. The function of repeats in the control region is still unclear, but it has been shown that repetitive sequences in some animal groups (shrews, cats and sheep) may include parts of ETAS1 and ETAS2 conservative blocks of mitochondrial DNA.

Methods and results

Analysis of the control region sequences for 31 Myotis petax specimens allowed the identification of the inter-individual variability and clarification of the composition of the R1-repeats. The copy number of the R1-repeats varies from 4 to 7 in individuals. The specimens examined do not exhibit a size heteroplasmy previously described for Myotis species. The unusual short 30 bp R1-repeats have been detected in M. petax for the first time. The ten specimens from Amur Region and Primorsky Territory have one or two copies of these additional repeats.

Conclusions

It was determined that the R1-repeats in M. petax control region consist of parts of the ETAS1 and ETAS2 blocks. The origin of the additional repeats seems to be related to the 51 bp deletion in the central part of the R1-repeat unit and subsequent duplication. Comparison of repetitive sequences in the control region of closely-related Myotis species identified the occurrence of incomplete repeats also resulting from the short deletions, but distinct from additional repeats of M. petax.

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Data availability

The datasets generated and analysed during the current study are available in the GenBank repository.

Change history

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Acknowledgements

We wish to express sincere gratitude to the Dr I.V. Kartavtseva for help in collecting and analysing the material. We are especially grateful to the members of Vladivostok Caving Club, to O.N. Morozov from the Geological and Speleological Club "Dolgan", to S.Yu. Ignatenko and E.V. Ignatenko from the Zeya Nature Reserve and to employees of Komsomolsk Nature Reserve for the organisation of field works.

Funding

This work was supported by RFBR according to the research project No. 18-34-00285 and by RSF according to the research project No. 22-24-00017. The research was carried out within the state assignment of Ministry of Science and Higher Education of the Russian Federation (Theme No. 121031500274-4).

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Authors and Affiliations

  1. Federal Scientific Center of the East Asia Terrestrial Biodiversity Far Eastern Branch of Russian Academy of Sciences, 159 Prospect Stoletiya St., Vladivostok, 690022, Russia

    Uliana Vasilievna Gorobeyko, Irina Nikolaevna Sheremetyeva & Valentin Yurievich Guskov

  2. Institute of Environmental and Agricultural Biology (X-BIO), Tyumen State University, 6 Volodarskogo St., Tyumen, 625003, Russia

    Denis Vasilievich Kazakov

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  1. Uliana Vasilievna Gorobeyko
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by UVG, DVK and VYG. The first draft of the manuscript was written by UVG and INS commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Uliana Vasilievna Gorobeyko.

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The authors have no relevant financial or non-financial interests to disclose.

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All applicable international, national and institutional ethics statements when using animals in research have been followed. Approval was granted by the Commission for the Regulation of Experimental Research (Bioethics Commissions) of Federal Scientific Center of the East Asia Terrestrial Biodiversity (Date 25.04.2022/No. 1).

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The original online version of this article was revised: In Table 1, the coordinates "43°17.133' N 133°36.800' E" is corrected as "43°16.200' N 133°37.200' E" and the coordinates "54°28.488′ N 113° 47.703′" is corrected as "54°26.400' N 113°46.800' E". In the acknowledgments section, the names "S.Yu. Ignateko and E.V. Igantenko” are corrected as "S.Yu. Ignatenko and E.V. Ignatenko".

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Gorobeyko, U.V., Sheremetyeva, I.N., Kazakov, D.V. et al. A new type of tandem repeats in Myotis petax (Chiroptera, Vespertilionidae) mitochondrial control region. Mol Biol Rep 50, 5137–5146 (2023). https://doi.org/10.1007/s11033-023-08468-4

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