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Biochemical and SSR based molecular characterization of elite rice varieties for straw lignocellulose

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Abstract

Background

Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources. Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.

Methods and results

Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting. For genotyping, 13 cellulose synthase specific polymorphic markers were used. The diversity analysis was performed using TASSEL 5.0 and GenAlE × 6.51b2, software program. Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels. The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128). A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system. The dendrogram analysis grouped the rice genotypes into two principal clusters i.e., cluster I and II. Cluster-II is monogenetic, while cluster-I is having 42 genotypes.

Conclusions

The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms. The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties. The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation. This study helped in identification of suitable dual purpose rice varieties for biofuel production without compromising food security.

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Acknowledgements

This work was supported by the Bharat Petroleum Corporation Limited, New-Delhi, India.

Funding

This study was supported by the Bharat Petroleum Corporation Limited, New-Delhi, India.

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Authors and Affiliations

  1. Department of Plant Breeding and Genetics, College of Agriculture, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, India

    Abinash Mishra, Jyoti Prakash Sahoo, Tapash Kumar Mishra, Sanat Kumar Dwibedi, Bandita Jena, Banshidhar Pradhan & Manasi Dash

  2. Department of Chemistry, Indian Institute of Technology, Roorkee, Uttarakhand, India

    Bishnupriya Swain

  3. College of Agriculture Engineering and Technology, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, India

    Spandan Nanda, Manas Ranjan Parida & Mahendra Kumar Mohanty

  4. College of Basic Science and Humanities, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, India

    Pradip Kumar Jena, Saubhagya Manjari Samantaray & Debiprasad Samantaray

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  1. Abinash Mishra
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Contributions

“All authors contributed to the study conception and design. Conceptualization and data interpretation was done by Abinash Mishra and Manasi Dash. Material preparation, conduct of experiment, data collection and analysis were performed by Abinash Mishra, Jyoti Prakash Sahoo, Bishnupriya Swain and Spandan Nanda. The first draft of the manuscript was written by Abinash Mishra and checked by Manasi Dash. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.”

Corresponding author

Correspondence to Manasi Dash.

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Mishra, A., Sahoo, J.P., Swain, B. et al. Biochemical and SSR based molecular characterization of elite rice varieties for straw lignocellulose. Mol Biol Rep 50, 5535–5545 (2023). https://doi.org/10.1007/s11033-023-08454-w

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  • DOI: https://doi.org/10.1007/s11033-023-08454-w

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