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Identification and morpho-molecular characterization of low spore strain in oyster mushroom

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Abstract

Background

Sporocarps of oyster mushroom liberate enormous spores and cause allergic reactions to workers involved in its cultivation. These spore-related allergies include stiffness or pain in the forearms, limbs, itchy throat, grogginess, and respiratory problems and are major problems during oyster mushroom cultivation.

Methods and results

In this study, we have generated seven hybrids using single-spore isolates (SSIs) of Pleurotus ostreatus var. florida (DMRP-49) and P. ostreatus (DMRP-30). Chimera was observed during cultivation trial of these hybrids and led to the development of low spore-producing/sporeless strain (DMRP-395) as evident from spore print and microscopic analysis. Further, the cultivation trial of this sporeless strain revealed a bunchy fruiting pattern and required 20–24 °C temperature for fruiting. At par yield was observed in sporeless strain. Notably, a prominent infundibuliform-shaped pileus along with central attachment of stipe was observed in the sporeless strain. Moreover, genetic diversity and principal component biplot analysis revealed resemblance of sporeless strain with one of the parental strain, i.e., P. ostreatus var. florida (DMRP-49).

Conclusions

The developed sporeless strain (DMRP-395) contains high protein and at par yield as compared with the control (DMRP-136). This sporeless strain will be helpful to reduce spore-related allergic responses in mushroom growers.

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Data availability

The datasets generated during and/or analyzed during the current study are available.

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Acknowledgements

The authors wish to thank Director, ICAR‒Directorate of Mushroom Research Chambaghat Solan for providing laboratory facilities and financial assistance under project

Funding

I thank Indian council of Agricultural research for the funding of the study.

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Authors and Affiliations

  1. ICAR-Directorate of Mushroom Research, Solan, 173213, India

    Anupam Barh, Shwet Kamal, Ved Parkash Sharma, Kanika Sharma, Babita Kumari & Manoj Nath

  2. ICAR-Indian Institute of Soil and Water Conservation, Dehradun, 248195, India

    Anupam Barh

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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by AB, SK, BK, KS, and MN. The first draft of the manuscript was written by VPS and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Anupam Barh.

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Barh, A., Kamal, S., Sharma, V.P. et al. Identification and morpho-molecular characterization of low spore strain in oyster mushroom. Mol Biol Rep 50, 5029–5038 (2023). https://doi.org/10.1007/s11033-023-08452-y

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