Abstract
Background
Malnutrition affects large section of population worldwide. Vitamin A and protein deficiencies have emerged as the major global health-issue. Traditional shrunken2 (sh2)-based sweet corn is deficient in provitamin A (proA), lysine and tryptophan. Natural variant of β-carotene hydroxylase1 (crtRB1) and opaque2 (o2) enhances proA, lysine and tryptophan in maize. So far, no sweet corn hybrid rich in these nutrients has been released elsewhere. Development of biofortified sweet corn hybrids would help in providing the balanced nutrition.
Methods and Results
We targeted three sh2-based sweet corn inbreds (SWT-19, SWT-20 and SWT-21) for introgression of mutant crtRB1 and o2 genes using molecular breeding. The gene-based 3′TE-InDel and simple sequence repeat (SSR) (umc1066) markers specific to crtRB1 and o2, respectively were utilized in foreground selection in BC1F1, BC2F1 and BC2F2. Segregation distortion was observed for crtRB1 and o2 genes in majority of populations. Background selection using 91–100 SSRs revealed recovery of recurrent parent genome (RPG) up to 96%. The introgressed progenies possessed significantly higher proA (13.56 µg/g) as compared to the original versions (proA: 2.70 µg/g). Further, the introgressed progenies had accumulated moderately higher level of lysine (0.336%) and tryptophan (0.082%) over original versions (lysine: 0.154% and tryptophan: 0.038%). Kernel sweetness among introgressed progenies (17.3%) was comparable to original sweet corn (17.4%). The introgressed inbreds exhibited higher resemblance with their recurrent parents for yield and morphological characters.
Conclusion
These newly developed biofortified sweet corn genotypes hold immense promise to alleviate malnutrition.
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Data availability
All the data sets supporting the conclusion of this article are included within the article, and its supporting information files are provided as accompanying supplementary materials.
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Acknowledgements
We thank WNC, IIMR, Hyderabad for providing the off-season nursery.
Funding
We are thankful to the ICAR-IARI, New Delhi for financial support.
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Conduct of the experiment: BS, Generation of backcross populations: RUZ, Recording of phenotypic data: GC and VB, Estimation of quality parameters: NG, Statistical analysis: VM, Writing of manuscript: BS, GC and FH, Designing of experiment: FH and SS.
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Singh, B., Zunjare, R.U., Shrivastava, S. et al. Provitamin A, lysine and tryptophan enrichment in shrunken2-based sweet corn genotypes through genomics-assisted breeding for crtRB1 and opaque2 genes. Mol Biol Rep 50, 4965–4974 (2023). https://doi.org/10.1007/s11033-023-08446-w
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DOI: https://doi.org/10.1007/s11033-023-08446-w