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Physiological and gene expression responses involved in teak (Tectona grandis L.) seedlings exposed to osmotic and salt stressors

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Abstract

Background

Teak (Tectona grandis L.) is a forest tree having 2n = 2x = 36 diploid chromosomes. Plants are continually subjected to variety of abiotic stresses due to climate change, which alter their physiological processes and gene expression.

Methods and results

The current study sought to examine the physiological and differential gene expression of teak seedlings exposed to abiotic stresses (150 mM NaCl and 15% PEG-6000). Chlorophyll content, membrane stability index and relative water content were measured at 0, 2, 7 and 12 days after treatment. These parameters were initially numerically reduced, but they were significantly reduced during a longer period of treatment. Seedlings treated with 150 mM NaCl displayed more harmful effect on the plant than other treatments. The results showed that variety of stresses significantly affect the physiology of seedlings because they cause membrane damage, ROS generation, chlorophyll degradation, and reduction in water absorption. The gene expression of treated and control seedlings was also evaluated at 12 days after treatment. Ten stress-related genes were examined for their differential expression using RT-PCR under applied stress. The stress-treated seedlings' leaves showed an up-regulated expression of the genes MYB-3, HSP-1, BI-1 and CS-2.

Conclusion

Up-regulation of the genes confirmed the protective function of these genes in plants under abiotic stress. However, gene expression was affected by treatments, the extent of stress and the species of plant. This study came to the conclusion that physiological parameters could be utilized as marker indices to assess a tree's capability to withstand stress at seedling stage. The up-regulated genes will be further investigated and utilized to validate stress tolerance and susceptible teak seedlings.

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Abbreviations

BI:

BAX inhibitor

CRD:

Completely random design

CS:

Cellulose synthases

CT:

Threshold cycle

CXEs:

Carboxylesterase

DAT:

Days after treatment

EC:

Electrical conductivity

HSP:

Heat shock protein

MSI:

Membrane stability index

MYB:

Myeloblastosis

NaCl:

Sodium chloride

NTC:

Non-template control

PAL:

Phenylalenine ammonia lyase

PEG:

Polyethylene glycol

RIN:

RNA integrity number

ROS:

Reactive oxygen species

RT-PCR:

Real time PCR

RWC:

Relative water content

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Authors and Affiliations

  1. Anand Agricultural University, Anand, India

    Hemanshukumar J. Maisuria, Haresh L. Dhaduk, Sushil Kumar, Amar A. Sakure & Amarjeet S. Thounaojam

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  1. Hemanshukumar J. Maisuria
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Maisuria, H.J., Dhaduk, H.L., Kumar, S. et al. Physiological and gene expression responses involved in teak (Tectona grandis L.) seedlings exposed to osmotic and salt stressors. Mol Biol Rep 50, 4875–4886 (2023). https://doi.org/10.1007/s11033-023-08437-x

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