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Association of glutathione-S-transferase polymorphism with genetic damage in paint workers

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Abstract

Background

Occupational exposure to toluene causes serious health problems ranging from drowsiness to lethal diseases such as cancer. Paint workers are exposed to toluene through inhalation or the dermal route, which can induce genetcic damage. The increased DNA damage could be linked to genetic polymorphism. Therefore, we evaluated the association of glutathione-S-transferase polymorphism with DNA damage in paint workers.

Methods

First, we included skilled paint workers (n = 30) as exposed and healthy individuals (n = 30) as control belonging to the same socio-economic strata. The genotoxicity biomarkers, Cytokinesis-block micronucleus (CBMN), and single-cell gel electrophoresis (SCGE)/Comet assay were used to assess genotoxicity while Multiplex-PCR and PCR-RFLP were used to assess polymorphism in glutathione-s-transferase (GST) genes. Using linear curve regression analysis, we assessed the association between genetic damage and polymorphism in the glutathione-s-transferase (GST) gene in the exposed and control subjects.

Results

A significantly higher frequency of CBMN (4.43 ± 1.50) and tail moment (TM) (11.23 ± 1.0) respectively in paint workers as compared to the control(1.50 ± 0.86 and (0.54 ± 0.37) underlined significantly high genetic damage in paint workers.Regression curve analysis reveals that polymorphism in the GST gene is significantly associated with higher MN and TM in paint workers.

Conclusion

Overall, our study provides a strong rationale for identifying a clear association between glutathione-S-transferase polymorphism and genetic damage in paint workers.

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Data availability

Data will be available from 1st author only at reasonable request.

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Authors and Affiliations

  1. Department of Life Sciences, SBSR, Sharda University, Greater Noida, 201310, UP, India

    KanuPriya

  2. Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, Haryana, India

    Sunil Kumar & Anita Yadav

  3. Department of Biochemistry, Kurukshetra University, Kurukshetra, 136119, Haryana, India

    Ranjan Gupta

  4. Department of Microbiology, Kurukshetra University, Kurukshetra, 136119, Haryana, India

    Neeraj Aggarwal

  5. Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, 110608, India

    Sunil Kumar

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  1. KanuPriya
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  4. Neeraj Aggarwal
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  5. Anita Yadav
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Contributions

Concept, design, and overall supervision: AY; Sample collection, processing, and data acquisition: KP; Analysis or interpretation of data: KP, AY, SK, NA, and RG; Drafting of the manuscript: KP and SK; Editing of the manuscript: KP, SK, AY, RG, and NA; All authors provided comments on the manuscript and evaluated critically. All authors gave their consent for publication.

Corresponding author

Correspondence to Anita Yadav.

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The authors declare no potential conflict of interest.

Ethical approval

All research procedures utilized in this study involving human participants were as per the ethical standards of the Institutional Human Ethics Committee of Kurukshetra University (IEC/12/240) and informed consent was obtained from all participants involved in the study.

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Informed consent was obtained from all individual participants included in the study.

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KanuPriya, Kumar, S., Gupta, R. et al. Association of glutathione-S-transferase polymorphism with genetic damage in paint workers. Mol Biol Rep 50, 4899–4905 (2023). https://doi.org/10.1007/s11033-023-08335-2

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  • DOI: https://doi.org/10.1007/s11033-023-08335-2

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