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Stem cell induction and plant regeneration are affected by medium components in maca (Lepidium meyenii Walp)

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Abstract

Background

In medicinal plants, selection, reproduction and preservation of important genotypes are very necessary. Nowadays, using tissue culture and regeneration techniques of medicinal plants under in vitro conditions has been able to proliferate medicinal plants widely, which is much higher than traditional methods of vegetative propagation. Maca (Lepidium meyenii), is an industrial plant whose root is the usable part. Maca has valuable medicinal effects such as sexual enhancement and reproductive power, infertility treatment, improved sperm count and quality, anti-stress, osteoporosis prevention and more.

Methods and results

This study was conducted to induce callus and regeneration of Maca. First, MS medium supplemented with different concentrations of Kinetin, Naphthaleneacetic acid and 2,4-Dichlorophenoxyacetic acid [0.5, 1 and 2 µM respectively] and control were compared for callus induction from root and leaves. After 38 days of incubation, the first callus appeared, after 50 days of callus induction and after 79 days regeneration occurred. The callus induction experiment was performed for the study of the effect of three explants (leaf, stem and root) and seven hormone levels. The regeneration experiment was carried out by studying the effect of three explants (leaf, stem and root) on eight levels of the hormone. The results of data analysis on callus induction showed that the effects of explants, hormones and their interactions on callus induction percentage were highly significant but not significant on callus growth rate. The results of regression analysis showed that explants, hormones and their interactions had no significant effect on regeneration percentage.

Conclusion

Based on our results, the best medium for inducing callus was Hormone 2,4-D [2 µM] and Kinetin [0.5 µM], in which the highest percentage of callus induction was in leaf explants (62%). And the lowest were in stem (30%) and root (27%) explants. According to the comparison of the mean, the best environment for regeneration of the environment was 4 µM 6-Benzylaminopurine 2.5 + Thidiazuron, in which the highest percentage of regeneration was in leaf explant (87%) and stem (69%) and the lowest in root explant (12). %).

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Acknowledgements

We would like to thank Razi University for providing the facilities for this research.

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Authors and Affiliations

  1. Department of Plant Production and Genetics, Faculty of Agriculture, Razi University, Kermanshah, Iran

    Danial Kahrizi, Atefeh Fahimi Far & Zeinab Chaghakaboodi

  2. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mozafar Khazaei

  3. Department of Medicinal and Aromatic Plants, Sivas Vocational School, Cumhuriyet University, Sivas, 58140, Turkey

    Esra Ucar Sozmen

  4. Department of Plant and Animal Production, Yozgat Vocational School, Yozgat Bozok University, Yozgat, Turkey

    Hulya Dogan

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  1. Danial Kahrizi
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  2. Atefeh Fahimi Far
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  3. Zeinab Chaghakaboodi
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  4. Mozafar Khazaei
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Contributions

Atefeh Fahimi Far, Zeinab Chaghakaboodi, Mozafar Khazaei: Execution research project, Experimental design, Data analysis, Manuscript preparation.

Danial Kahrizi, Esra Ucar Sozmen, Hulya Dogan: Experimental design, Data analysis, Manuscript preparation.

Corresponding author

Correspondence to Danial Kahrizi.

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Conflict of Interest

Danial Kahrizi, Atefeh Fahimi Far, Zeinab Chaghakaboodi, Mozafar Khazaei, Esra Ucar Sozmen and Hulya Dogan declare that she does not have a conflict of interest. Danial Kahrizi declares that he does not have a conflict of interest.

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This study was approved by the Ethics Committee of Razi University, Kermanshah, Iran.

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Kahrizi, D., Far, A.F., Chaghakaboodi, Z. et al. Stem cell induction and plant regeneration are affected by medium components in maca (Lepidium meyenii Walp). Mol Biol Rep 50, 4187–4192 (2023). https://doi.org/10.1007/s11033-023-08319-2

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