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Evaluation of T helper17 as skeletal homeostasis factor in peripheral blood mononuclear cells and T helper cells of end-stage renal disease cases with impaired parathyroid hormone

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Abstract

Background

Chronic renal failure is mainly connected with high and low parathyroid hormone (PTH) levels and immunological impairments. The present study aimed to evaluate T helper 17 (Th17) cells as a crucial modulator of the immune system and skeletal homeostasis in hemodialysis patients with impaired intact PTH (iPTH).

Methods

In this research, blood samples were taken from ESRD patients with high (> 300 pg/mL), normal (150–300 pg/mL), and low (< 150 pg/mL) serum intact parathyroid hormone (iPTH( levels (n = 30 in each group). The frequency of Th17 (CD4+ IL17+) cells was evaluated by flow cytometry in each group. The expression levels of Th17 cell-related master transcription factors, cytokines in peripheral blood mononuclear cells (PBMC), and Th cells, and the level of the mentioned cytokines were determined in the supernatant of PBMCs.

Results

The number of Th17 cells remarkably increased in subjects with high iPTH against low and normal iPTH. Also, RORɣt and STAT3 levels were significantly higher in high iPTH ESRD patients than in other groups in the expression of mRNA and protein levels. These findings are confirmed by evaluating the IL-17 and IL-23 in the supernatant of cultured PBMCs and isolated Th cells.

Conclusion

Our findings indicated that increased serum PTH levels in hemodialysis cases may be involved in increasing the differentiation of CD4 + cells to Th17 cells in PBMC.

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Acknowledgements

This work is part of the dissertation of Dr. Khadijeh Fereydoonzadeh (Grant No. 64316), supported by the Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

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Authors and Affiliations

  1. Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

    Roza Motavalli & Mohammadreza Sadeghi

  2. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Roza Motavalli, Mehdi Yousefi & Jalal Etemadi

  3. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Sadegh Soltani-Zangbar

  4. Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Sadegh Soltani-Zangbar & Mehdi Yousefi

  5. Kidney Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Khadijeh fereydoonzadeh & Bahram Niknafs

  6. Behbahan Faculty of Medical Sciences, Behbahan, Iran

    Mahsa Hajivalili

  7. Department of Allergy and Clinical Immunology, Pediatric Hospital, Tabriz University of Medical Sciences, Tabriz, Iran

    Javad Ahmadian Heris

  8. Department of Toxicogenomics, GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht, The Netherlands

    Houman Kahroba

  9. Center for Environmental Sciences, University of Hasselt, Agoralaan D, BE-3590, Hasselt, Belgium

    Houman Kahroba

  10. Department of Virology, Pasteur Institute of Iran, Tehran, Iran

    Farhad Motavalli Khiavi

  11. Physical Medicine and Rehabilitation Research Center, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran

    Sanam Dolati

  12. Department of Nephrology, Tabriz University of Medical Sciences, Tabriz, Iran

    Jalal Etemadi

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  1. Roza Motavalli
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  2. Mohammad Sadegh Soltani-Zangbar
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  8. Farhad Motavalli Khiavi
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Correspondence to Mehdi Yousefi or Jalal Etemadi.

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Motavalli, R., Soltani-Zangbar, M., fereydoonzadeh, K. et al. Evaluation of T helper17 as skeletal homeostasis factor in peripheral blood mononuclear cells and T helper cells of end-stage renal disease cases with impaired parathyroid hormone. Mol Biol Rep 50, 4097–4104 (2023). https://doi.org/10.1007/s11033-023-08306-7

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  • DOI: https://doi.org/10.1007/s11033-023-08306-7

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