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Neuroprotective effect of a novel brain-derived peptide, HIBDAP, against oxygen-glucose deprivation through inhibition of apoptosis in PC12 cells

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Abstract

Background

The effect of a novel brain-derived peptide, hypoxic-ischemic brain damage associated peptide (HIBDAP), on apoptosis after oxygen-glucose deprivation (OGD) in PC12 cells was investigated.

Methods

The HIBDAP sequence (HSQFIGYPITLFVEKER) was coupled with the carrier peptide of the transactivator of transcription (TAT) sequence (YGRKKRRQRRR). FITC-labelled TAT-HIBDAP was observed by fluorescence microscopy. After TAT-HIBDAP treatment and OGD treatment, the PC12 cell apoptosis rate was analysed using lactate dehydrogenase (LDH) leakage and Annexin V-fluorescein isothiocyanate (FITC) assays. Mitochondrial membrane potential (ΔΨm) was examined by fluorescence microscopy. Protein expression of apoptotic factors was examined by Western blotting.

Results

FITC-labelled TAT-HIBDAP entered the PC12 cell nucleus. Compared with the OGD group, TAT-HIBDAP at low concentrations (1 µM, 5 µM, 10 µM) significantly reduced the apoptosis rate of PC12 cells (except at 20 µM); 5 µM TAT-HIBDAP had the most obvious effect. There were remarkable increases in ΔΨm at different concentrations (1 µM, 5 µM, 10 µM, 20 µM) of TAT-HIBDAP pretreatment, and 5 µM TAT-HIBDAP also had the most obvious effect. TAT-HIBDAP reversed the increased ratio of Bax/Bcl-2 and activation of Caspase-3 induced by OGD.

Conclusion

TAT-HIBDAP is resistant to OGD-induced PC12 cell apoptosis by regulating the Bax/Bcl-2/Caspase-3 pathway, which may provide a novel therapeutic strategy for neonatal HIBD.

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Data availability

All supporting data are included within the main article.

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Acknowledgements

This project was supported by the National Natural Science Foundation of China (No. 81671500), 333 project of Jiangsu Province, and Nanjing Sanitation Engineering of Young Talents during the 13th Five-Year Plan Period (QRX17076).

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Author notes

  1. Chenhong Jiang and Yina Hu have contributed equally to this work.

Authors and Affiliations

  1. Nanjing Medical University, Nanjing, China

    Chenhong Jiang & Yina Hu

  2. Dapartment of Paediatrics, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China

    Xuewen Hou

  3. Department of Neonatology, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, China

    Jie Qiu

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  1. Chenhong Jiang
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  2. Yina Hu
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Contributions

JQ conceptualized and designed the study, coordinated and supervised the experiments, provided research materials/reagents, reviewed and revised the manuscript. XH conducted the experiments and data interpretation. CJ and YH did the experiments, collected data, analyzed data and drafted the initial manuscript. All authors read and approved the final manuscript.

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Correspondence to Xuewen Hou or Jie Qiu.

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Jiang, C., Hu, Y., Hou, X. et al. Neuroprotective effect of a novel brain-derived peptide, HIBDAP, against oxygen-glucose deprivation through inhibition of apoptosis in PC12 cells. Mol Biol Rep 50, 3045–3051 (2023). https://doi.org/10.1007/s11033-023-08248-0

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  • DOI: https://doi.org/10.1007/s11033-023-08248-0

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