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Polymorphisms in vitamin D receptor genes and its relation with susceptibility to brucellosis: a case-control study

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Abstract

Objective

One of the systemic infections is Brucellosis which is caused by facultative intracellular bacteria of the genus Brucella. Vitamin D is a fat-soluble prohormone, that metabolizes enzymes and its intracellular receptor creates the active hormone and also mediate in responses of immune system.

Methods

Current research consists of 102 patients with brucellosis who were selected based on culture, PCR results serology, and clinical symptoms. The control group composed of 102 healthy people. The polymorphism of genes (Bsm I, Fok I, Taq I, Apa I) encoding Vitamin D receptor (VDR) were assessed by the PCR-RFLP method.

Results

The results showed that ff, tt, aa, and bb genotypes in Fok I, ApaI, TaqI, and BsmI were significant in case/control groups (P-value ≤ 0.0001). The genotype frequency AA in the control group is higher than that of the study group, while genotype frequency aa in the study group is more than the control. The odds ratio for brucellosis in individuals with ff genotype is 37 times higher than that of Ff genotype. Also, the odds ratio of brucellosis in individuals with genotype tt, aa, and bb was 12, 53, and 6 times higher than those of the Aa, Bb, and Tt genotypes.

Conclusion

The genotypes aa and ff in the positions of the ApaI and FokI are of higher importance. The brucellosis risk in individuals accompanied aa genotype at Apa I is 53 times higher than that of the genotype AA, in other words, AA and BB, TT and FF genotypes are protective against the disease.

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Data availability

The data presented in this study are available on request from the corresponding author.

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Acknowledgements

The authors wish to express their appreciation to the Vice-Chancellor of Research and Technology of Hamadan University of Medical Sciences, Hamadan, Iran. We also sincerely thank Professor Ali Reza Soltanian, Department of Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran, for his help in data analysis.

Funding

This study was granted by the Vice-Chancellor of Research and Technology of Hamadan University of Medical Sciences, Hamadan, Iran (Grant No: 9603302218).

Author information

Authors and Affiliations

  1. Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Hassan Mahmoudi & Mohammad Yousef Alikhani

  2. Nahavand School of Allied Medical Sciences,, Hamadan University of Medical Sciences, Hamadan, Iran

    Hassan Mahmoudi

  3. Infectious Disease Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

    Fariba keramat & Mohammad Yousef Alikhani

  4. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Massoud Saidijam

  5. Department of Biostatistics and Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran

    Younes Mohammadi

  6. University of Brighton, Brighton, UK

    Amir Khodavirdipour

Authors
  1. Hassan Mahmoudi
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  2. Fariba keramat
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  6. Mohammad Yousef Alikhani
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Contributions

MYA and FK cooperated in the design of the study and selecting the patients. HM and YM contributed to sampling and data analysis. MYA and AK prepared the first draft. MYA and MS contributed to preparing and revising the manuscript. MYA and HM contributed to revising the manuscript. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Mohammad Yousef Alikhani.

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Conflicts of interest

The authors declared no conflict of interest.

Ethical approval

The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Ethics Committee of Hamadan University of Medical Sciences, Hamadan, Iran (IR.UMSHA.REC.1396.266).

Informed consent

Informed consent was obtained from all subjects involved in the study.

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Cite this article

Mahmoudi, H., keramat, F., Saidijam, M. et al. Polymorphisms in vitamin D receptor genes and its relation with susceptibility to brucellosis: a case-control study. Mol Biol Rep 50, 2077–2083 (2023). https://doi.org/10.1007/s11033-022-08195-2

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  • DOI: https://doi.org/10.1007/s11033-022-08195-2

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