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Altered microbiota profile in seminal vesicles of men presenting with refractory hematospermia

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Abstract

Background

Currently, no recognized evidence is known about the bacterial communities found within seminal vesicles (SV) of men presenting with refractory hematospermia.

Methods and results

Fifteen male patients with refractory hematospermia or anejaculation were enrolled, and 15 SV-Infection (SV-In) samples from SV with hemorrhage and/or stones, 11 SV-Control (SV-C) samples from SV with non-infection, and 14 Urine (Urine) samples from posterior urethra were obtained via transurethral seminal vesiculoscopy. Then the high-throughput 16 S rRNA gene sequencing method was performed to characterize the microbiota profile. Finally, a total of 1535 operational taxonomic units (OTUs) were found, 1295 OTUs were shared across three groups, 7 OTUs, 45 OTUs, and 48 OTUs were unique to SV-C group, SV-In group, and Urine group, respectively. The 5 top bacterial phyla (mean relative abundance) in all samples were Firmicutes (52.08%), Bacteroidetes (21.69%), Proteobacteria (12.72%), Actinobacteria (9.64%), and Fusobacteria (1.62%), the 5 top bacterial genera in all samples were Bacteroides (9.13%), Lactobacillus (5.38%), Bifidobacterium (5.35%), Faecalibacterium (5.10%), and Allobaculum (3.34%), of which Bifidobacterium had the highest level in SV-C samples and had a significant difference (P < 0.05) across all groups. Differential analysis showed genera Leuconostoc and LachnospiraceaeFCS020group were identified as biomarkers in the SV-In microbiota.

Conclusion

Altered microbiota composition in seminal vesicles is related to refractory hematospermia in men, and the distribution of genus Leuconostoc or LachnospiraceaeFCS020group within seminal vesicles may interact with hematospermia. This study provides clues for the diagnosis and treatment of this urologic disorder.

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Data availability

16 S rRNA gene sequence data for each sample have been deposited at NCBI’s Sequence Read Archive (SRA; BioProject ID: PRJNA892056).

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Acknowledgements

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Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Department of Urology, Beijing Chao-Yang Hospital, Capital Medical University, Institute of Urology, Capital Medical University, No. 8 Gongren Tiyuchang Nanlu, Chaoyang District, 100020, Beijing, China

    Hongen Lei, Hu Han, Xiaodong Zhang & Long Tian

  2. Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin Medical University, 300211, Tianjin, China

    Yuhong Feng & Zhongcheng Xin

Authors
  1. Hongen Lei
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  2. Hu Han
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  4. Xiaodong Zhang
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  6. Long Tian
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Contributions

Conceptualization, H.L. and L.T.; Methodology, H.L., H.H., and Y.F.; Formal Analysis, H.L., H.H., and Y.F.; Investigation, H.L.; Writing - Original Draft, H.L.; Writing - Review & Editing, X.Z., Z.X., and L.T.; Visualization, X.Z. and Z.X.; Supervision, L.T.

Corresponding author

Correspondence to Long Tian.

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Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Beijing Chao-Yang Hospital, Capital Medical University (No. 2021-KE-482).

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Informed consent was obtained from all individual participants included in the study.

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Lei, H., Han, H., Feng, Y. et al. Altered microbiota profile in seminal vesicles of men presenting with refractory hematospermia. Mol Biol Rep 50, 2381–2389 (2023). https://doi.org/10.1007/s11033-022-08139-w

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  • DOI: https://doi.org/10.1007/s11033-022-08139-w

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