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Genome-wide identification and expression analysis of metal tolerance protein (MTP) gene family in soybean (Glycine max) under heavy metal stress

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Abstract

Aim

Plant metal tolerance proteins (MTPs) are plant membrane divalent cation transporters that specifically contribute to heavy metal stress resistance and mineral uptake. However, little is known about this family’s molecular behaviors and biological activities in soybean.

Methods and results

A total of 20 potential MTP candidate genes were identified and studied in the soybean genome for phylogenetic relationships, chromosomal distributions, gene structures, gene ontology, cis-elements, and previous gene expression. Furthermore, the expression of MTPs has been investigated under different heavy metals treatments. All identified soybean MTPs (GmaMTPs) contain a cation efflux domain or a ZT dimer and are further divided into three primary cation diffusion facilitator (CDF) groups: Mn-CDFs, Zn-CDFs, and Fe/Zn-CDFs. The developmental analysis reveals that segmental duplication contributes to the GmaMTP family’s expansion. Tissue-specific expression profiling revealed comparative expression profiling in similar groups, although gene expression differed between groups. GmaMTP genes displayed biased responses in either plant leaves or roots when treated with heavy metal. In the leaves and roots, nine and ten GmaMTPs responded to at least one metal ion treatment. Furthermore, in most heavy metal treatments, GmaMTP1.1, GmaMTP1.2, GmaMTP3.1, GmaMTP3.2, GmaMTP4.1, and GmaMTP4.3 exhibited significant expression responses.

Conclusion

Our findings provided insight into the evolution of MTPs in soybean. Overall, our findings shed light on the evolution of the MTP gene family in soybean and pave the path for further functional characterization of this gene family.

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Acknowledgements

The authors are grateful to and acknowledge the Sichuan Province Government for providing such a well-equipped platform for conducting research, the management of Yibin University for their support and for providing us with a pleasant environment for research, and the Chinese Government and the Chinese public in general for their love of Science and Research. We thank Yibin University’s High-level Talent Project for its assistance during the experimental investigation.

Funding

We would like to thank the Sichuan Provincial Government, as well as Yibin University's High-level Talent Project (No.2018RC07), for their assistance throughout the experimental investigation.

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Author notes

  1. Ahmed H. El-Sappah, Manzar Abbas, Shabir A. Rather and Huang Qiulan have contributed equally to this work.

Authors and Affiliations

  1. School of Agriculture, Forestry, and Food Engineering, Yibin University, Yibin, Sichuan, China

    Ahmed H. El-Sappah, Manzar Abbas, Huang Qiulan & Jia Li

  2. Genetics Department, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt

    Ahmed H. El-Sappah & Ahmed S. Eldomiaty

  3. Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, 666303, Mengla, Yunnan, China

    Shabir A. Rather

  4. Mountain Research Centre for Field Crops, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, Anantnag-192 101, Khudwani, J&K, India

    Shabir H. Wani

  5. Department of Crop Science, Facultyof Agriculture, Zagazig University, Zagazig, 44511, Egypt

    Nourhan Soaud

  6. School of Chemical Engineering Beijing Institute of Technology, Beijing, China

    Zarqa Noor

  7. Division of Genetics and Plant Breeding, Faculty of Agriculture (FoA), SKUAST–Kashmir, Wadura, Sopore, Kashmir, India

    Reyazul Rouf Mir

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  1. Ahmed H. El-Sappah
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Contributions

Conceptualization: AHES, MA, HQ and JL. Formal analysis: AHES, JL, SHW and HQ. Writing an original draft: AHES. Methodology: AHES, ASE, ZN and NS. Writing, review and editing; AHES, MA, SAR, SHW, MB and JL. Investigation: AHES, HQ and RRM.

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Correspondence to Jia Li.

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Ahmed H. El-Sappah, Manzar Abbas, Shabir A. Rather and Huang Qiulan share the first authorship. Shabir A. Rather

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El-Sappah, A.H., Abbas, M., Rather, S.A. et al. Genome-wide identification and expression analysis of metal tolerance protein (MTP) gene family in soybean (Glycine max) under heavy metal stress. Mol Biol Rep 50, 2975–2990 (2023). https://doi.org/10.1007/s11033-022-08100-x

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