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Vanillic acid potentiates insulin secretion and prevents pancreatic β-cells cytotoxicity under H2O2-induced oxidative stress

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Abstract

Background

Oxidative stress is known to impair cellular functions and, therefore, plays a significant role in the pathophysiology of various diseases, including diabetes. The persistently elevated glucose levels may cause enhanced mitochondrial reactive oxygen species generation, which in turn can damage the pancreatic β-cells. In this study, we have investigated the effect of vanillic acid on preventing H2O2-induced β-cells death and retaining its insulin secretion potentiating effect in the presence of H2O2.

Methods

The insulin secretion from the BRIN-BD11 cells was quantified using ELISA-based assays. The viability of the cells was assessed by estimated by the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) colorimetric assay and DAPI staining. The expression levels of apoptotic and antioxidant proteins were estimated by western blot experiments.

Results

Vanillic acid protected pancreatic β-cells viability and function under the H2O2 oxidative stress condition. The Erk1/2 activation appears to play an important role in vanillic acid potentiated insulin secretion and protection of the β-cells in the presence of H2O2. Vanillic acid pretreated cells exhibited enhanced expression of antioxidant enzymes such as catalase and SOD-2 and reduced the expression of proapoptotic markers such as BAX and BAD. In addition, it also enhanced the expression of oxidative stress-sensitive transcription factor Nrf-2 and cell survival protein Akt.

Conclusion

The present study shows that vanillic acid potentiates insulin secretion and protects pancreatic β-cells from H2O2-induced oxidative stress.

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Acknowledgements

The authors thank CSIR-CFTRI for the funding of the project. Also, all the authors also acknowledge the Academy of Scientific and Innovative Research (AcSIR) and Director, CSIR-CFTRI, Mysuru, for constant support to carry out this work.

Funding

This work has been supported financially by the CSIR- Central Food Technological Research Institute (CFTRI) MLP-247 Project.

Author information

Author notes

  1. Chandan Muddahally Naganna, K. Yogendra Prasad, and V. P. Mahendra Prasad have equal contribution to this work.

Authors and Affiliations

  1. Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute (CFTRI), Mysuru, Karnataka, 570020, India

    Chandan Muddahally Naganna, K. Yogendra Prasad, V. P. Mahendra & Ravi Kumar

  2. Department of Biochemistry & Biotechnology, CSIR-Central Leather Research Institute Adyar, Chennai, 600020, India

    P. Ganesan

  3. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    K. Yogendra Prasad, V. P. Mahendra, P. Ganesan & Ravi Kumar

Authors
  1. Chandan Muddahally Naganna
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  2. K. Yogendra Prasad
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Contributions

MNC carried out cell culture, protein expression, and insulin secretion assays analyzed the data and wrote the manuscript under the supervision of RK. YPK Conducted the experiments of microscopy and protein expression studies. MVP conducted experiments on ROS estimation and protein expression studies and wrote the manuscript under the supervision of RK: PG helped with protein expression studies and reviewed the manuscript and RK: Conceived the idea, supervised the project, analyzed the data, and wrote the manuscript. All the authors approved the final version of the manuscript.

Corresponding author

Correspondence to Ravi Kumar.

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Naganna, C.M., Prasad, K.Y., Mahendra, V.P. et al. Vanillic acid potentiates insulin secretion and prevents pancreatic β-cells cytotoxicity under H2O2-induced oxidative stress. Mol Biol Rep 50, 1311–1320 (2023). https://doi.org/10.1007/s11033-022-08046-0

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