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High percentage of Cancer Stem cells in metastatic locations: Upregulation of cicBIRC6 in highly metastatic breast Cancer Subline

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Abstract

Background

Metastasis is a devastating complication of breast cancer. Cancer relapse and metastasis are associated with cancer stem cells. CicBIRC6 is a circular RNA that is proposed to be involved in the stemness of stem cells. In breast cancer, metastatic tumor cells have higher stem cell properties. In the present study, we evaluate the expression of cicBIRC6 in these cells.

Methods

After the development of a syngeneic animal model of TNBC, primary breast cancer cells named 4T1T were isolated from the tumor mass. Highly metastatic tumor cells named 4T1B and 4T1L were isolated and expanded from brain metastasis lesions and lungs of cancerous mice respectively. Sphere formation ability in metastatic and primary tumor cells was evaluated separately. The quantitative real-time polymerase chain reaction was performed to analyze the expression of cicBIRC6 in primary and metastatic tumor cells.

Results

Our data revealed that, sphere formation ability among metastatic tumor cells was significantly higher. Surprisingly expression of cicBIRC6 was significantly upregulated in these metastatic tumor cells. In comparison with 4T1T, cicBIRC6 was upregulated 5.7 and 3.5 times in 4T1B and 4T1L respectively.

Conclusion

These findings provided important insights regarding the molecular properties of metastatic tumor cells and can be used for designing a targeted therapeutic strategy in combat with these cells.

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Data Availability

All data generated or analyzed during this study are included in this published article.

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Acknowledgements

We would like to thank the research assistant of Shahroud University of medical sciences and all the participants who helped us in this project.

Funding

This study was supported by grant No 140082 from Shahroud University of Medical Sciences (SHMU).

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Authors and Affiliations

  1. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

    Mohammad Kamalabadi-Farahani

  2. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Roqaye Karimi

  3. Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran

    Amir Atashi

Authors
  1. Mohammad Kamalabadi-Farahani
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  2. Roqaye Karimi
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  3. Amir Atashi
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Corresponding author

Correspondence to Mohammad Kamalabadi-Farahani.

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The author declares that they have no competing interests.

Ethical statement

The Ethics Committee of Shahroud University of Medical Sciences approved this study for ethics in animal research (registration number: IR.SHMU.REC.1400.265).

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Kamalabadi-Farahani, M., Karimi, R. & Atashi, A. High percentage of Cancer Stem cells in metastatic locations: Upregulation of cicBIRC6 in highly metastatic breast Cancer Subline. Mol Biol Rep 50, 1303–1309 (2023). https://doi.org/10.1007/s11033-022-08024-6

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  • DOI: https://doi.org/10.1007/s11033-022-08024-6

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