Abstract
Background
Thyroid hormones play a vital function in the maturation in the course of mind development. Regarding the well-known effects of choline on brain-derived neurotrophic factor (BDNF), the study examined the effects of choline on hippocampal BDNF gene expression in maternal hypothyroidism rats.
Methods and results
To induce the hypothyroidism, 6-propyl-2-thiouracil was introduced to the ingesting water from the sixth day of gestation to twenty-first postnatal day (PND). Choline-treatment started twice a day on the first day of gestation until PND 21. On PND28, pups were sacrificed. The expression of BDNF gene was evaluated after the hippocampus was harvested. Our results demonstrated that both male and female pre-pubertal offspring rats’ BDNF gene expression was decreased by developmental hypothyroidism. Choline increases the ratio of relative gene expression of BDNF in the hippocampus of males and females in the control/hypothyroidism group, especially in males.
Conclusions
It can be concluded that maternal choline supplementation on the first day of gestation until PND 21 improves brain development and cognitive function in pre-pubertal offspring rats regarding control/hypothyroidism groups.
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Acknowledgements
The authors are grateful to all staff of Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran.
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SSH, LD, HS, ES, and MB contributed in study design, data analysis and interpretation, and drafting of manuscript; SSH, LD, and MB contributed in interpretation of results and critical revision of the manuscript.
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Ethics committee of Urmia Medical Sciences University approved all stages of the study (IR.UMSU.REC.1397.449).
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Sheikhi, S., Derafshpour, L., Aghazadeh, R. et al. Effects of choline supplementation in mothers with hypothyroidism on the brain-derived neurotrophic factor gene expression changes in pre-pubertal offspring rats. Mol Biol Rep 50, 2351–2356 (2023). https://doi.org/10.1007/s11033-022-08014-8
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DOI: https://doi.org/10.1007/s11033-022-08014-8