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The LncRNA MIAT is identified as a regulator of stemness-associated transcript in glioma

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Abstract

Background

Myocardial infarction-associated transcript (MIAT) is a long non-coding RNA (lncRNA) with altered expression in different diseases and malignancies. In this study, the potential expression and function of lncRNA MIAT in intuition and progression of brain cancer was investigated.

Methods and Results

At first, TCGA data analysis demonstrated that lncRNA MIAT is significantly upregulated in various malignancies, especially its expression is dramatically elevated in brain tumors. In line with the data, we further evaluated the expression of MIAT in a series of brain tumor tissue, and our results revealed that the expression of MIAT was noticeably overexpressed in glioblastoma (p = < 0.0001). We further found that the expression of MIAT was markedly upregulated in low-grade brain tumors rather than high-grade ones. To further investigate the biological function of MIAT in brain cancer cells, its expression was suppressed by si-RNA-mediated knocking down. Inhibition of MIAT resulted in reduced proliferation of brain tumor cells followed by cell cycle arrest at the G1 phase, and significant induction of apoptosis, and senescence, but limited the migration ability and epithelial-mesenchymal-transition (EMT). Moreover, knocking-down of MIAT reduced the expression of stemness factors, followed by upregulation of their downstream miRNAs (micro RNAs), let-7a-5p, and miR-29b-3p.

Conclusions

Altogether, our data demonstrated that lncRNA MIAT could control proliferation, migration, and metastasis of brain cancer cells via regulating the Nanog/ Sox2 / let-7a-5p / miR-29b-3p axis. This data could introduce lncRNA MIAT as a novel oncogene in brain cancer pathogenesis.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This study was supported by the Graduate University of Advanced Technology, Kerman, and the Department of Research of the University of Isfahan, Iran. All biological materials were provided by the IRAN NATIONAL TUMOR BANK, which is funded by the Cancer Institute of Tehran University for Cancer Research.

Funding

This study was supported by the Graduate University of Advanced Technology, Kerman, and Department of Research of the University of Isfahan, Iran.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology and Microbiology, Faculty of Science and Technology, University of Isfahan, Isfahan, Iran

    Farzane Amirmahani & Sadeq Vallian

  2. Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

    Malek Hossein Asadi

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  1. Farzane Amirmahani
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  2. Sadeq Vallian
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Contributions

All authors contributed to the study conception and design. Material preparation and data collection were performed by Farzane Amirmahani, Sadeq Vallian, and Malek Hossein Asadi. Data analysis was performed by Farzane Amirmahani. The first draft of the manuscript was written by Farzane Amirmahani. Sadeq Vallian, and Malek Hossein Asadi commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Sadeq Vallian or Malek Hossein Asadi.

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This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Graduate University of Advanced Technology.

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Amirmahani, F., Vallian, S. & Asadi, M.H. The LncRNA MIAT is identified as a regulator of stemness-associated transcript in glioma. Mol Biol Rep 50, 517–530 (2023). https://doi.org/10.1007/s11033-022-07962-5

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