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Two missense variants of the epidermal growth factor receptor gene are associated with non small cell lung carcinoma in the subjects from Iraq

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Abstract

Background

Lung carcinoma is a foremost cause of cancer-related mortality worldwide. Variable genetic factors are associated with the development of lung cancer. This study was performed to evaluate the possible association of epidermal growth factor receptor (EGFR) gene polymorphisms with non small cell lung carcinoma (NSCLC) in Iraqi population.

Methods

DNA samples were extracted from 100 patients and 100 controls. Four PCR fragments were designed to amplify four high-frequency variants within EGFR, namely rs1050171, rs2072454, rs2227984, and rs2227983. The PCR fragments were genotyped by single-strand conformation polymorphism (SSCP) method, and each genotype was subjected to direct sequencing.

Results

Genotyping experiments confirmed the variability of three targeted variants, and logistic regression analysis showed that two of these variants (rs1050171 and rs2227983) tend to exhibit a significant association with NSCLC. Individuals with rs1050171:GA genotype showed a possible association with the increased risk of NSCLC (P = 0.0110; OD 5.2636; Cl95% 1.4630 to 18.9371). Individuals with rs2227983:GG genotype exhibited a potential association with NSCLC (P = 0.0037; OD 5.2683; Cl95% 1.7141 to 16.1919). Linkage disequilibrium analysis showed that the effects of the investigated variants seem to take independent actions, and no haplotype was found to be associated with the high prevalence of NSCLC.

Conclusions

Our collective data indicated that EGFR-rs1050171G/A and EGFR-rs2227983G/G SNPs tend to exert significant and separate associations with the increased risk of NSCLC. However, this study recommends using a broader spectrum of the investigated samples to get further details of both SNPs in terms of their association with the susceptibility to NSCLC.

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Data availability

The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available privacy and ethical restrictions, as stipulated by the University of Kufa Institutional Review Board.

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Acknowledgements

The authors would like to acknowledge the Middle-Euphrates Cancer Center (MECC), Najaf province, and Merjan Cancer Center (MCC), Babil province for granting the samples and providing informative scientific help to accompany the preliminary data preparations.

Funding

This work was not financially supported by any funding agency.

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Authors and Affiliations

  1. Department of Biology, College of Science, University of Kufa, Najaf, 54001, Iraq

    Zahraa K. Lawi

  2. Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, 51001, Iraq

    Mohammed Baqur S. Al-Shuhaib & Ahmed H. Alkhammas

  3. Laboratory of Medicinal and Environment Chemistry, Higher Institute of Biotechnology, University of SFAX, PB 261, 3000, Sfax, Tunisia

    Ibtissem Ben Amara

Authors
  1. Zahraa K. Lawi
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  2. Mohammed Baqur S. Al-Shuhaib
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  3. Ibtissem Ben Amara
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  4. Ahmed H. Alkhammas
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Contributions

ZKL developed the work, and conducted genotyping experiments. MBSA contributed to the supervision of the work, analyzed the data, and wrote the manuscript. TMA supervised the research. AHA participated in the genotyping experiments. All authors approved the final version of the work.

Corresponding author

Correspondence to Mohammed Baqur S. Al-Shuhaib.

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Conflict of interest

No conflict of interest is reported by the authors.

Ethical approval

The experiments performed in the study were conducted following the Helsinki Declaration for experiments involving people and the biochemical research involving human subjects was approved by the Institutional Review Board (IRB) in the University of Kufa (IECIH/UOK 088/2020; CAAE 08802212). Signed written informed consents were obtained from all participants before being involved in the study.

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Lawi, Z.K., Al-Shuhaib, M.B.S., Amara, I.B. et al. Two missense variants of the epidermal growth factor receptor gene are associated with non small cell lung carcinoma in the subjects from Iraq. Mol Biol Rep 49, 11653–11661 (2022). https://doi.org/10.1007/s11033-022-07933-w

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