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Overexpression of Zostera japonica 14-3-3 gene ZjGRF1 enhances the resistance of transgenic Arabidopsis to copper stress

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Abstract

Background

Copper is both a nutrient essential for plant growth and a pollutant. In recent decades, with the rapid development of industrial and agricultural production, copper has been used more and more widely, and its consumption has also increased rapidly. Excessive soil copper contents induce phytotoxicity, affecting plant growth, development and yields. Moreover, copper can accumulate in crops and enter the food chain through enrichment, harming human health.

Methods and results

In this study, Arabidopsis wild-type (WT) and Zostera japonica 14-3-3 gene ZjGRF1 overexpression lines were used to explore the physiological function and molecular mechanism of ZjGRF1 in Arabidopsis in the copper stress response. Under copper stress, compared with WT plants, transgenic ZjGRF1 Arabidopsis plants exhibited less inhibition of root growth and development and had higher fresh weights. Under copper stress, the soluble sugar and soluble protein contents in transgenic ZjGRF1 Arabidopsis plants were significantly higher than those in WT plants, while the superoxide dismutase (SOD), peroxidase and catalase (CAT) activities were significantly higher than those in WT plants. Additionally, the malonaldehyde content of transgenic plants was significantly lower than that of WT plants. Furthermore, qRT-PCR results showed that under copper stress, the SOD, CAT1 and HMA5 expression levels in transgenic ZjGRF1 Arabidopsis plants were significantly higher than those in WT plants, while COPT1 expression was significantly lower than that in WT plants.

Conclusions

ZjGRF1 enhanced the copper stress resistance of Arabidopsis by maintaining high antioxidant enzyme activity, increasing copper efflux and reducing copper uptake under copper stress.

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Data Availability

All data generated or analyzed during this study are included in this published article or supplementary file. The nucleotide and deduced amino acid sequence data of ZjGRF1 and ZjGRF2 were submitted to GenBank (No. MW199706, MW206765).

Code Availability

Not applicable.

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Acknowledgements

This study was funded by the Natural Science Foundation of Guangxi Province (2020GXNSFAA297067), the Research Fund Program of the Guangxi Key Lab of Mangrove Conservation and Utilization (GKLMC-21A01; GKLMC-20A04; GKLMC-20A01; 662), the National Natural Science Foundation of China (32170399) and the National Science & Technology Fundamental Resources Investigation Program of China (2019FY100604). These funding sources had no role in the study design, collection, analysis, interpretation of data and manuscript writing.

Funding

This study was funded by the Natural Science Foundation of Guangxi Province (2020GXNSFAA297067), the Research Fund Program of Guangxi Key Lab of Mangrove Conservation and Utilization (GKLMC-21A01; GKLMC-20A04; GKLMC-20A01), the National Natural Science Foundation of China (32170399) and the National Science & Technology Fundamental Resources Investigation Program of China (2019FY100604). These funding organizations had no role in the study design, collection, analysis, interpretation of data and manuscript writing.

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Authors and Affiliations

  1. Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, 536007, Beihai, Guangxi, China

    Siting Chen & Guanglong Qiu

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  1. Siting Chen
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  2. Guanglong Qiu
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Contributions

SC designed and conducted the experiment. GQ conducted field sampling and identification. SC and GQ wrote the manuscript. All the authors reviewed the manuscript.

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Correspondence to Siting Chen or Guanglong Qiu.

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Chen, S., Qiu, G. Overexpression of Zostera japonica 14-3-3 gene ZjGRF1 enhances the resistance of transgenic Arabidopsis to copper stress. Mol Biol Rep 49, 11635–11641 (2022). https://doi.org/10.1007/s11033-022-07915-y

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