Abstract
Background
Mannheimia haemolytica is one of the main agents of domestic pneumonic mannheimiosis, but a proper vaccine has not been explored in IRAN.
Methods and Results
362 lung and nasal samples from sick domestic animal were detected by culture and PCR methods. Totally, 71 M. haemolytica isolates were identified in three main serotypes (A1, A2, and A6). Serotypes A2 (38/71; 54%) and A1 (25/71; 39%) were the most frequently detected, whereas the A6 serotype was detected with a frequency of less than 1% (1/71; 1%) and 7 isolates remained unknown (7/71; 10%). Subsequently, M. haemolytica vaccinal strain was developed and then formalin-killed vaccine was prepared. It provided the best protection against mannheimiosis in sheep which was proved by indirect ELISA.
Conclusions
Our results suggest that the efficacy and safety of vaccine strain are remarkable and may serve as a new therapeutic target in mannheimiosis.
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Acknowledgements
We thank the Razi Vaccine and Serum Research Institute (RVSRI), Shiraz, Iran for their support and Mr. S. Sadeghzadeh (Lab Animal Department) for his technical assistance. This study was supported by the Iran National Science Foundation (INSF) and in part by Research Grant number 99013545.
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This study was supported by the Razi Vaccine and Serum Research Institute and in part by Grant number 99013545.
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All the authors read and approved the final manuscript. YT designed the research work; HM, MH and ES performed the experiments; and HM wrote the manuscript and submitted the assembled sequence of M. haemolytica.
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Animal owners provided their verbal informed consent for injection of vaccine as well as for animal blood sampling. Blood samples were collected by veterinarians according to the guidelines on animal husbandry. The study protocol was approved by Razi Vaccine and Serum Research Institute animal house.
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Molaee, H., Tahamtan, Y., Saeednezhad, E. et al. Isolation of the various serotypes of Mannheimia haemolytica and preparation of the first vaccine candidate in Iran. Mol Biol Rep 49, 10367–10375 (2022). https://doi.org/10.1007/s11033-022-07890-4
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DOI: https://doi.org/10.1007/s11033-022-07890-4