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SSR marker-based genetic characterization of Turkish oriental tobaccos

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Abstract

Background

Turkey is one of the traditional oriental tobacco producing countries. Oriental tobaccos produced in Turkey are ecotypes specific to certain regions, local varieties and landraces, and they have unique characteristics. The present study was conducted to reveal the general and interregional genetic diversity levels of tobacco genotypes collected from different regions of Turkey based on DNA markers.

Methods and Results

A total of 319 ecotypes/lines of Turkish Oriental Tobaccos collected from different regions (Aegean Region, Marmara Region, Karadeniz Region, and East and Southeast Anatolia Regions) of Turkey and cultivars Xanthi 81, Xanthi 2A, Canik Sitmasuyu, Katerini, Canik 190-5 and NC 55 were subjected to SSR marker analysis. Eighty-nine alleles were obtained from 21 markers examined, and the average number of alleles per marker was 4.05. A total of 314 unique tobacco genotypes were found among 319 plants evaluated using 19 polymorphic SSR markers. In the dendrogram constructed using SSR marker data, genotypes were divided into six clades based on geographic regions and levels of genetic diversity.

Conclusions

The presence of 314 unique genotypes among 319 tobacco plants evaluated indicated the high level of genetic diversity among Turkish tobaccos. While some of them were N. rustica tobaccos, some others were reminiscent of semi-oriental tobaccos. These genotypes merit further agronomic and technological characterizations, which could allow their utilization in future tobacco breeding.

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Data availability

The datasets generated in the present study are available from the authors upon request.

Code availability

Not applicable.

References

  1. Kurt D (2021) Impacts of environmental variations on quality and chemical contents of oriental tobacco. Contributions Tobacco Nicotine Res 30:50–62. https://doi.org/10.2478/cttr-2021-0006

    Article  CAS  Google Scholar 

  2. Peksuslu A, Yilmaz I, Inal A, Kartal H (2012) Tobacco genotypes. J Aegean Agric Res Inst 22:82–90

    Google Scholar 

  3. Karalar F (1996) The place of tobacco in Turkish economy and its importance in Aegean region and among provinces of the region. J Agric Fac Gaziosmanpasa Univ 1:91–101 ((in Turkish with English abstract))

    Google Scholar 

  4. Necip A, Isık M, Guzel A, Takim K, Kaygisiz F (2021) LC-MS/MS analysis, antioxidant properties and inhibition effect on some important metabolic enzymes of Nicotiana rustica L. KSU. J Agric Nat 24:930–938. https://doi.org/10.18016/ksutarimdoga.vi.856514

    Article  Google Scholar 

  5. Kandemir N, Yildirim A, Gunduz R (2010) Determining the levels of genetic variation using SSR markers in three Turkish barley materials known as Tokak. Turk J Agric For 34:17–23. https://doi.org/10.3906/tar-0903-22

    Article  CAS  Google Scholar 

  6. Bindler G, van der Hoeven R, Gunduz I, Plieske J, Ganal M, Rossi L, Gadani F, Donini P (2007) A microsatellite marker-based linkage map of tobacco. Theor Appl Genet 114:341–349. https://doi.org/10.1007/s00122-006-0437-5

    Article  CAS  PubMed  Google Scholar 

  7. Bindler G, Plieske J, Bakaher N, Gunduz I, Ivanov N, Van der Hoeven R, Ganal M, Donini P (2011) A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development. Theor Appl Genet 123:219–230. https://doi.org/10.1007/s00122-011-1578-8

    Article  PubMed  PubMed Central  Google Scholar 

  8. Tong Z, Yang Z, Chen X, Jiao F, Li X, Wu X, Gao Y, Xiao B, Wu W (2012) Large-scale development of microsatellite markers in Nicotiana tabacum and construction of a genetic map of flue-cured tobacco. Plant Breed 131:674–680. https://doi.org/10.1111/j.1439-0523.2012.01984

    Article  CAS  Google Scholar 

  9. Moon HS, Nifong JM, Nicholson JS, Heineman A, Lion K, van der Hoeven R, Hayes AJ, Lewis RS (2009) Microsatellite-based analysis of tobacco (Nicotiana tabacum L.) genetic resources. Crop Sci 49:2149–2159. https://doi.org/10.2135/cropsci2009.01.0024

    Article  CAS  Google Scholar 

  10. Darvishzadeh R, Basirnia A, Maleki HH, Jafari M (2014) Association mapping for resistance to powdery mildew in oriental tobacco (Nicotiana tabaccum L.) germplasm. Iran J Genet Plant Breed 3:21–30

    Google Scholar 

  11. Davalieva K, Maleva I, Filipovski K, Spiroski O, Efremov GD (2010) Genetic variability of Macedonian tobacco varieties determined by microsatellite marker analysis. Diversity 2:439–449. https://doi.org/10.3390/d2040439

    Article  CAS  Google Scholar 

  12. Keskin A, Koprulu TK, Bursali A, Ozsemir AC, Yavuz KE, Tekin S (2014) First record of Ixodes arboricola (Ixodida: Ixodidae) from Turkey with presence of Candidatus Rickettsia vini (Rickettsiales: Rickettsiaceae). J Med Entomol 51:864–867. https://doi.org/10.1603/ME13169

    Article  PubMed  Google Scholar 

  13. Sneath PHA, Sokal RR, Freeman WH (1975) Numerical taxonomy. The principles and practice of numerical classification. Syst Zool 24:263. https://doi.org/10.2307/2412767

    Article  Google Scholar 

  14. Fricano A, Bakaher N, Del Corvo M, Piffanelli P, Donini P, Stella A, Nikolai V, Ivanov NV, Pozz C (2012) Molecular diversity, population structure, and linkage disequilibrium in a worldwide collection of tobacco (Nicotiana tabacum L.) germplasm. BMC Genet 13:18. https://doi.org/10.1186/1471-2156-13-18

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Saygili I, Kinay A, Kandemir N (2020) Determination of an SSR marker set to distinguish genotypes of different tobacco classes. J Agric Fac Gaziosmanpasa Univ 37:102–108. https://doi.org/10.13002/jafag4677

    Article  Google Scholar 

  17. Kurt D, Yilmaz G, Kinay A (2021) GE interaction and stability analysis in some Basma type oriental tobacco (Nicotiana tabacum L.) lines. J Agric Sci 27:312–320. https://doi.org/10.15832/ankutbd.680854

    Article  Google Scholar 

  18. Ganesh CT, Saiprasad GVS, Mohan-Raju B, Sheshshayee MS, Udayakumar M (2014) Genetic structure in FCV tobacco population as assessed by multi-locus genotyping using SSR markers. Adv Crop Sci Tech 2:127. https://doi.org/10.4172/2329-8863.1000127

    Article  Google Scholar 

  19. Bernardo AN, Bowden RL, Rouse MN, Newcomb MS, Marshall DS, Bai G (2013) Validation of molecular markers for new stem rust resistance genes in U.S. hard winter wheat. Crop Sci 53:755–764. https://doi.org/10.2135/cropsci2012.07.0446

    Article  CAS  Google Scholar 

  20. Yaldiz G, Camlica M, Nadeem MA, Nawaz MA, Baloch FS (2018) Genetic diversity assessment in Nicotiana tabacum L. with iPBS-retrotransposons. Turk J Agric For 42:154–164

    Article  CAS  Google Scholar 

  21. Camas N, Karaali H, Kurt D, Kinay A (2011) Evaluation of quality factors for basma kind of tobacco production in middle Black Sea Region. IX. Field Crops Congress of Turkey pp 908–913.

  22. Demir K (2019) Tobacco agriculture and reji company in the period of the II Mesrutiyet (Constituonalism) in the Ottoman Press. Balikesir Univ J Soc Sci Ins 22(41):419–446. https://doi.org/10.31795/baunsobed.580791 (in Turkish with English abstract)

    Article  Google Scholar 

  23. Caliskan O, Kurt D, Kinay A, Camas N, Kevseroglu K (2018) Determination of genetic variability of eastern and southeastern Anatolia region tobaccos. J Agri Food Environ Sci 72(2):8–14

    Google Scholar 

  24. Kanoglu S, Kinay A, Karakoc E (2020) Determination of quality properties and applied agricultural practice in roll your own tobacco. Anadolu J Agric Sci 35(2):192–197. https://doi.org/10.7161/omuanajas.661631 (in Turkish with English abstract)

    Article  Google Scholar 

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Acknowledgements

The authors would like to acknowledge the Scientific and Technological Research Council of Turkey (TUBİTAK) for funding this research.

Funding

This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, Grant no; TOVAG 112O153).

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Authors and Affiliations

  1. Department of Field Crops, Faculty of Agriculture, Tokat Gaziosmanpasa University, Tokat, Turkey

    Ibrahim Saygili & Ahmet Kinay

  2. Department of Biology, Faculty of Sciences and Letters, Ankara Hacı Bayram Veli University, Polatlı, Ankara, Turkey

    Nejdet Kandemir

  3. Department of Field Crops, Faculty of Agriculture, Ondokuz Mayis University, Samsun, Turkey

    Selim Aytac & Ali Kemal Ayan

Authors
  1. Ibrahim Saygili
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  2. Nejdet Kandemir
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  3. Ahmet Kinay
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  4. Selim Aytac
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  5. Ali Kemal Ayan
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Contributions

NK, SA, and AKA conceptualized, designed the research. AK collected the ecotypes/lines. IS and NK performed experiments, analyzed data, and wrote the manuscript.

Corresponding author

Correspondence to Ibrahim Saygili.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Saygili, I., Kandemir, N., Kinay, A. et al. SSR marker-based genetic characterization of Turkish oriental tobaccos. Mol Biol Rep 49, 11351–11358 (2022). https://doi.org/10.1007/s11033-022-07815-1

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  • DOI: https://doi.org/10.1007/s11033-022-07815-1

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