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DNAH5 gene and its correlation with linc02220 expression and sperm characteristics

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Abstract

Background

Numerous pieces of evidence show that many environmental and genetic factors can cause male infertility. Much research in recent years has investigated the function of long non-coding RNAs (lncRNAs) in fertility. The main objective of the current study was to investigate the expression of Dynein Axonemal Heavy Chain 5 (DNAH5) as a gene that plays an essential role in sperm motility in individuals with asthenozoospermia and terato-asthenozoospermia. Alterations in linc02220 expression (located close to the DNAH5 gene), its action potential in DNAH5 regulating, and the correlation between their expression and normal sperm morphology and motility were also examined.

Method and material:

This study examined the semen of 31 asthenozoospermia individuals (AZ), 33 terato-asthenozoospermia (TAZ) individuals, and 33 normospermia (NZ) individuals with normal sperm as a control group. The expression levels of DNAH5 and linc02220 in the sperm samples were analyzed by real-time PCR.

Results

Gene expression analysis revealed a significant association between DNAH5 expression and sperm motility and morphology (p < 0.0001). The DNAH5 expression levels in the TAZ and AZ groups were also significantly reduced; however, linc02220 was significantly upregulated in both TAZ and AZ groups compared to the NZ group (p < 0.0001). DNAH5 expression in the TAZ and AZ groups was negatively correlated with linc02220 expression, thus, DNAH5 downregulation was associated with linc02220 overexpression (p < 0.05).

Conclusions

The gene linc02220 could be a potential regulatory target for DNAH5, and both could affect sperm’s normal motility and morphology.

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Data Availability

The datasets analyzed during the current study are available from the corresponding author by his decision on reasonable request.

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Acknowledgements

We gratefully thank the Research and Technology Deputy of Shahrekord University of Medical Sciences for their support.

Funding

This research was supported by the Shahrekord University of Medical Sciences (Grant no. 3181).

Author information

Authors and Affiliations

  1. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Ali Kamel, Mohammadreza Saberiyan, Samaneh Adelian & Hossein Teimori

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  1. Ali Kamel
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  2. Mohammadreza Saberiyan
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  3. Samaneh Adelian
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  4. Hossein Teimori
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Contributions

AK and MS designed the study, carried out data collection and manuscript drafting. SA contributed to data analysis and manuscript drafting. HT supervised the study and manuscript editing. All authors approved the final version for publication.

Corresponding author

Correspondence to Hossein Teimori.

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Ethical approval and Consent to participate

The study was approved by Research Ethics Committee of Shahrekord University of Medical Sciences (ethics code: IR.SKUMS.REC.1398.155). All the patients signed written informed consent to participate in the study. The Declaration of Helsinki was considered in this study.

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The authors declare that they have no known conflict of interests or personal relationships that could have appeared to influence the work reported in this paper.

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Kamel, A., Saberiyan, M., Adelian, S. et al. DNAH5 gene and its correlation with linc02220 expression and sperm characteristics. Mol Biol Rep 49, 9365–9372 (2022). https://doi.org/10.1007/s11033-022-07787-2

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