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Serine O-acetyltransferase derived NV14 peptide reduces cytotoxicity in H2O2 induced MDCK cells and inhibits MCF-7 cell proliferation through caspase gene expression

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A Correction to this article was published on 18 April 2023

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Abstract

Background

Most of the bioactive peptides exhibit antioxidant effect and do elicit inhibitory effect on proliferation of cancer cells. This study investigates the in-vitro antioxidant and anti-cancer properties of NV14 peptide, derived from serine O-acetyltransferase (SAT) of spirulina, Arthrospira platensis.

Methods

The anti-cancer effect of the peptide was evaluated using human adenocarcinoma epithelial cells (MCF-7), while the anti-oxidant potential, as in reduction in ROS concentration, has been established using the H2O2-exposed, Madin-Darby canine kidney (MDCK) cells. The outcome of the in vitro analyses has been evaluated by in silico molecular docking analyses.

Results

The peptide, dose-dependently, reduced oxidative stress as well as cell proliferation. Besides, based on the binding scores between NV14 peptide and the important proteins associated with apoptosis and antioxidant defense, it is evident that the peptide has antioxidant and anti-cancer effect, in vitro.

Conclusions

Together, this study demonstrates that NV14 has a potent antioxidant and anti-cancer capability; however, further direction needs to be focused on clinical or pharmacodynamics aspects.

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Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Code Availability

Not applicable.

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Authors and Affiliations

  1. Department of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, 603 203, Kattankulathur, Chennai, Tamil Nadu, India

    Manikandan Velayutham, B. Haridevamuthu, P. Snega Priya & Jesu Arockiaraj

  2. Department of Chemistry, Interdisciplinary Institute of Indian System of Medicine, SRM Institute of Science and Technology, 603 203, Kattankulathur, Chennai, Tamil Nadu, India

    Munuswamy Ramanujam Ganesh

  3. Foundation for Aquaculture Innovations and Technology Transfer (FAITT), Thoraipakkam, Chennai, 600 097, Tamil Nadu, India

    Annie Juliet

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Contributions

MV and JA contributed to the concept and design of the study; MV. BH. MRG performed the experiments; AJ and JA contributed significantly to resources, data analysis, manuscript preparation and perform the analysis with constructive discussions; JA supervised and checked the manuscript; All authors read and approved the final manuscript

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Correspondence to Jesu Arockiaraj.

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The original online version of this article was revised: The affiliation of the author "Annie Juliet" is corrected as "Foundation for Aquaculture Innovations and Technology Transfer (FAITT), Thoraipakkam, Chennai 600 097, Tamil Nadu, India."

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Velayutham, M., Haridevamuthu, B., Priya, P.S. et al. Serine O-acetyltransferase derived NV14 peptide reduces cytotoxicity in H2O2 induced MDCK cells and inhibits MCF-7 cell proliferation through caspase gene expression. Mol Biol Rep 49, 9205–9215 (2022). https://doi.org/10.1007/s11033-022-07746-x

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