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Dual roles of ANGPTL4 in multiple inflammatory responses in stomatitis mice

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Abstract

Background

Stomatitis is inflammation of the oral mucosa. Angiopoietin-like protein 4 (ANGPTL4) has pleiotropic functions both anti-inflammatory and pro-inflammatory properties. In the present study, we tested whether there is a correlation between increased ANGPTL4 expression and inflammation in stomatitis mice and the mechanisms involved.

Methods and Results

In this study, the oral mucosa of mice was burned with 90% phenol and intraperitoneal injection of 5-fluorouracil to establish the model of stomatitis mice. The pathological changes of stomatitis mice were observed by H&E staining of paraffin section. The expressions of cytokines and ANGPTL4 were detected by fluorescence quantitative PCR, and the protein levels of ANGPTL4 were detected by western blot. Compared with control group, the oral mucosal structure of model mice was damaged. The expression of ANGPTL4 were significantly increased concomitantly with elevated production of anti-inflammatory cytokine (peroxisome proliferator-activated receptor alpha) and pro-inflammatory cytokines [nuclear transcription factor-kappa B, interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α] in mice with stomatitis.

Conclusions

This study suggests that ANGPTL4 may be a double-edged sword in multiple inflammatory responses in stomatitis mice.

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All authors made sure that all data and materials as well as software application or custom code support their published claims and comply with field standards.

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Funding

This study was supported by Hunan Provincial Natural Science Foundation of China (14JJ2079).

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Authors and Affiliations

  1. College of Veterinary Medicine, Hunan Agricultural University, Furong District, Changsha, 410128, China

    Miao-Miao Tian, Yi-Shan Wang & Hong-Bo Xiao

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  1. Miao-Miao Tian
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  2. Yi-Shan Wang
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Contributions

All authors contributed to the design of the study, analysis and discussion of the results, and review and revision of the manuscript.

Corresponding author

Correspondence to Hong-Bo Xiao.

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The authors declare that they have no competing interest.

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Approval was obtained from Hunan Agricultural University ethics review board.

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Tian, MM., Wang, YS. & Xiao, HB. Dual roles of ANGPTL4 in multiple inflammatory responses in stomatitis mice. Mol Biol Rep 49, 9195–9204 (2022). https://doi.org/10.1007/s11033-022-07745-y

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  • DOI: https://doi.org/10.1007/s11033-022-07745-y

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