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Strawberry crinkle (SCV) and Strawberry mottle (SMoV) viruses affect yield and physio-biochemical responses of three strawberry cultivars under different seasonal conditions

Molecular Biology Reports (2022)Cite this article

Abstract

Background

The current study was performed to evaluate the impact of Strawberry crinkle virus (SCV) and Strawberry mottle virus (SMoV) viruses, in single and mixed infections, on the yield and physio-biochemical traits of strawberry plants with different genetic backgrounds. Accordingly, the virus-free plantlets were produced via meristem culture and were then transplanted in a research field in different seasons.

Methods and results

The results indicated that genetic background clearly affects the responses of the strawberry plants to the viral infection. In addition, considering the effects of changing environmental factors, different seasons had significant influences on the strawberry responses to the virus infection. The results indicated that the components indicating destructive effects were significantly higher during the summer season. Reverse transcription–polymerase chain reaction using viral specific primers revealed 345 and 461 bp fragments for SCV and SMoV viruses, respectively, where all plants were positive for studied viruses. The comparison between the influence of SCV and SMoV viruses on the host plants indicated that SCV caused more damaging effects on the strawberry fruit yield and physio-biochemical traits. Simultaneous infection with SCV and SMoV severely impacted the strawberry plants compared with the effect of each virus individually.

Conclusion

It is recommended to keep the temperature of the greenhouses producing strawberry at the cooler levels to prevent the spreading of the aphid-transmitted viruses.

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References

  1. Hancock JF (2020) Strawberries, 2nd/Ed. CABI, New York

    Google Scholar 

  2. Abad CFA, Álvarez LSJ, Mora EDC (2020) Effect of the roof (Low tunnel) on the productivity of two varieties of strawberry (Fragaria vesca) in Cajanuma, Loja. La Granja: Revista de Ciencias de la Vida 31:131–141

    Article  Google Scholar 

  3. Yosefi A, Mozafari AA, Javadi T (2020) Jasmonic acid improved in vitro strawberry (Fragaria× ananassa Duch.) resistance to PEG-induced water stress. Plant Cell Tissue Organ Cult 142:549–558

    CAS  Article  Google Scholar 

  4. Dedejani S, Ali-Akbar M, Ghaderi N (2021) Salicylic acid and iron nanoparticles application to mitigate the adverse effects of salinity stress under in vitro culture of strawberry plants. Iran J Sci Technol Trans A Sci 45:821–831

    Article  Google Scholar 

  5. Hoseiny M, Mozafari AA, Nazari F (2021) The antagonistic relationships between salicylic acid and simvastatin on phyto-biochemical components in Hymenocrater longiflorus Benth under in vitro conditions. Ind Crops Prod 167:113556

    CAS  Article  Google Scholar 

  6. Martin RR, Tzanetakis IE (2006) Characterization and recent advances in detection of strawberry viruses. Plant Dis 90:384–396

    Article  Google Scholar 

  7. Shakeri E, Mozafari A, Sohrabi F, Saed-Moucheshi A (2019) Role of proline and other osmoregulatory compounds in plant responses to abiotic stresses. Handbook of plant and crop stress, fourth edition. CRC Press, New York, pp 165–173

    Chapter  Google Scholar 

  8. Saed-Moucheshi A, Pakniyat H, Pirasteh-Anosheh H, Azooz M (2014) Role of ROS as signaling molecules in plants. In: Ahmad P (ed) Reactive oxygen species, antioxidant network and signaling in plants. Springer Publication, New York, pp 585–626

    Google Scholar 

  9. Riasat M, Kiani S, Saed-Mouchehsi A, Pessarakli M (2019) Oxidant related biochemical traits are significant indices in triticale grain yield under drought stress condition. J Plant Nutr 42:111–126

    CAS  Article  Google Scholar 

  10. Saed-Moucheshi A, Fasihfar E, Hasheminasab H, Rahmani A, Ahmadi A (2013) A review on applied multivariate statistical techniques in agriculture and plant science. Intl J Agron Plant Prod 4:127–141

    Google Scholar 

  11. Saed-Moucheshi A, Pessarakli M, Mozafari AA, Sohrabi F, Moradi M, Marvasti FB (2021) Screening barley varieties tolerant to drought stress based on tolerant indices. J Plant Nutr. https://doi.org/10.1080/01904167.2021.1963773

    Article  Google Scholar 

  12. Tabarzad A, Ayoubi B, Riasat M, Saed-Moucheshi A, Pessarakli M (2017) Perusing biochemical antioxidant enzymes as selection criteria under drought stress in wheat varieties. J Plant Nutr 40:2413–2420

    CAS  Article  Google Scholar 

  13. Vosough A, Ghouchani R, Saed-Moucheshi A (2015) Genotypic variation and heritability of antioxidant related traits in wheat landraces of Iran. Res Trends 7(2):43–47

    Google Scholar 

  14. He C, Gao D, Fan L, Xu T, Xing F, Li S, Wang H (2021) First report of Strawberry virus 1 infecting strawberry in China. Plant Dis. https://doi.org/10.1094/PDIS-01-21-0038-PDN

    Article  PubMed  Google Scholar 

  15. Li C, Yamagishi N, Kasajima I, Yoshikawa N (2019) Virus-induced gene silencing and virus-induced flowering in strawberry (Fragaria× ananassa) using apple latent spherical virus vectors. Hortic Res 6:1–10

    Article  Google Scholar 

  16. Torrico AK, Salazar SM, Kirschbaum DS, Conci VC (2018) Yield losses of asymptomatic strawberry plants infected with Strawberry mild yellow edge virus. Eur J Plant Pathol 150:983–990

    Article  Google Scholar 

  17. Ghaderi Zandan N, Hajizadeh M (2019) Molecular identification of strawberry mottle virus in strawberry fields of Kurdistan Province based on the 3΄NCR. Appl Entomol Phytopathol 87(1):107–116 (In Persian with English abstract)

    Google Scholar 

  18. Hajizadeh M (2018) Molecular detection and phylogenetic determinant of strawberry crinkle virus in strawberry fields of Kurdistan Province based on the partial polymerase: gene sequences. J Appl Res Plant Prot 7(2):96–105 (In Persian with English abstract)

    Google Scholar 

  19. Nasirinia Gh, Pourrahim R, Elahinia SASA, Rouhibakhsh AA, Farzadfar Sh (2016) Incidence of strawberry important virus diseases in Guilan and Mazandaran provinces. Appl Entomol Phytopathol 84(1):31–42

    Google Scholar 

  20. Murashige TC, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantar 15:473–497

    CAS  Article  Google Scholar 

  21. Mozafari AA, Havas F, Ghaderi N (2018) Application of iron nanoparticles and salicylic acid in in vitro culture of strawberries (Fragaria× ananassa Duch.) to cope with drought stress. Plant Cell Tissue Organ Cult 132:511–523

    CAS  Article  Google Scholar 

  22. Mazzara M, James DJ (2000) The influence of photoperiodic growth condition on isolation of RNA from strawberry (Fragaria × ananassa Duch.) tissue. Mol Biotechnol 15:237–241

    CAS  Article  Google Scholar 

  23. Thompson JR, Wetzel S, Klerks M, Vašková D, Schoen C, Špak J, Jelkmann W (2003) Multiplex RT-PCR detection of four aphid-borne strawberry viruses in Fragaria spp. in combination with a plant mRNA specific internal control. J Virol Methods 111:85–93

    CAS  Article  Google Scholar 

  24. Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: Measurement and characterization by UV-VIS spectroscopy. Curr Protoc Food Anal Chem 1:f4.3.1-F4.3.8

    Article  Google Scholar 

  25. Bates L, Waldren R, Teare I (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207

    CAS  Article  Google Scholar 

  26. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    CAS  Article  Google Scholar 

  27. Pick E, Mizel D (1981) Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader. J Immunol Methods 46:211–226

    CAS  Article  Google Scholar 

  28. Ahmad R, Tripathi AK, Tripathi P, Singh S, Singh R, Singh RK (2008) Malondialdehyde and protein carbonyl as biomarkers for oxidative stress and disease progression in patients with chronic myeloid leukemia. In Vivo 22:525–528

    PubMed  Google Scholar 

  29. Irigoyen J, Einerich D, Sánchez-Díaz M (1992) Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plant 84:55–60

    CAS  Article  Google Scholar 

  30. Flohé L, Günzler WA (1984) [12] Assays of glutathione peroxidase. Method Enzymol 105:114–120

    Article  Google Scholar 

  31. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    CAS  Article  Google Scholar 

  32. Fránová J, Přibylová J, Koloniuk I (2019) Molecular and biological characterization of a new strawberry cytorhabdovirus. Viruses 11:982

    Article  Google Scholar 

  33. Zhai Y, Yuan Q, Qiu S, Li S, Li M, Zheng H, Wu G, Lu Y, Peng J, Rao S (2021) Turnip mosaic virus impairs perinuclear chloroplast clustering to facilitate viral infection. Plant Cell Environ 34:1–16

    Google Scholar 

  34. Zhao J, Zhang X, Hong Y, Liu Y (2016) Chloroplast in plant-virus interaction. Front Microbiol 7:1565

    PubMed  PubMed Central  Google Scholar 

  35. Yadav D, Rana R (2020) Transmission of plant virus through arthropod vector. J Entomol Zool Stud 8:1934–1939

    Google Scholar 

  36. Jeger MJ (2020) The epidemiology of plant virus disease: towards a new synthesis. Plants 9:1768

    CAS  Article  Google Scholar 

  37. Fan L, He C, Wu M, Gao D, Dong Z, Hou S, Feng Z, Wang H (2021) Incidence, genomic diversity, and evolution of strawberry mottle virus in China. Biocell 45:1137

    CAS  Article  Google Scholar 

  38. Saed-Moucheshi A, Sohrabi F, Fasihfar E, Baniasadi F, Riasat M, Mozafari AA (2021) Superoxide dismutase (SOD) as a selection criterion for triticale grain yield under drought stress: a comprehensive study on genomics and expression profiling, bioinformatics, heritability, and phenotypic variability. BMC Plant Biol 21:1–19

    Article  Google Scholar 

  39. Ammar E-D, Tsai C-W, Whitfield AE, Redinbaugh MG, Hogenhout SA (2009) Cellular and molecular aspects of rhabdovirus interactions with insect and plant hosts. Annu Rev Entomol 54:447–468

    CAS  Article  Google Scholar 

Download references

Acknowledgements

We thank Research Center of Strawberry Breeding and Improvement, University of Kurdistan, for excellent technical assistance.

Funding

This work was supported by the University of Kurdistan under Grant number GRC96-00228-2.

Author information

Authors and Affiliations

  1. Department of Horticultural Sciences, Faculty of Agriculture, University of Kurdistan, P. O. Box: 416, Sanandaj, 66177-15175, Iran

    Ali Akbar Mozafari, Yavar vafaee & Shahla Ghaderi

  2. Research Center of Strawberry Breeding and Improvement, University of Kurdistan, 14, Sanandaj, 66177-15175, Iran

    Ali Akbar Mozafari

  3. Department of Plant Protection Faculty of Agriculture, University of Kurdistan, P. O. Box: 416, Sanandaj, 66177-15175, Iran

    Mohammad Hajizadeh & Nasrin Ghaderi Zandan

  4. Department of Agronomy and Plant Breeding, University of Kurdistan, P. O. Box: 416, Sanandaj, 66177-15175, Iran

    Paghah Shahidi

  5. Horticulture and Crop Research Department, Kermanshah Agricultural & Natural Resources Research & Education Center (AREEO), Kermanshah, Iran

    Armin Saed-Moucheshi

Authors
  1. Ali Akbar Mozafari
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  2. Yavar vafaee
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  3. Mohammad Hajizadeh
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  4. Armin Saed-Moucheshi
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  6. Nasrin Ghaderi Zandan
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Contributions

In this experiment AAM designed and supervised experiment. YV and PS performed experiment and assembled data. Data analysis has been performed by ASM, NGZ and SGH. The draft manuscript has been written by AAM and MH.

Corresponding author

Correspondence to Ali Akbar Mozafari.

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Conflict of interest

The authors declare no conflict of interest. The funders had no role in the design of the study.

Ethical approval

The experimental protocol in this study was established according to the ethical guidelines and were approved with the number 97004045-2-1on 17 January 2019 by the Plant Protection Care and Use Committee of the University of Kurdistan, Sanandaj, Iran.

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Mozafari, A.A., vafaee, Y., Hajizadeh, M. et al. Strawberry crinkle (SCV) and Strawberry mottle (SMoV) viruses affect yield and physio-biochemical responses of three strawberry cultivars under different seasonal conditions. Mol Biol Rep (2022). https://doi.org/10.1007/s11033-022-07701-w

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  • DOI: https://doi.org/10.1007/s11033-022-07701-w

Keywords

  • Aphid-transmitted virus
  • Viral disease
  • Biplot
  • Meristem culture
  • Strawberry variety