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Characterization of Mungbean yellow mosaic India virus genome with a recombinant DNA-B in Southern Peninsular India.

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Abstract

Background

Mungbean yellow mosaic India virus (MYMIV) is a representative of the genus begomovirus/Begomoviridae, which is prevalent in the northern part of Indian subcontinent causing yellow mosaic disease (YMD). This virus is rapidly evolving and breaking the resistance in the advanced lines causing huge economic losses in the pulse production. In this context, the present investigation on characterization of the causal organism of YMD was undertaken

Methods and results

A novel recombinant isolate (YMV-BG-BPT) causing YMD was identified from blackgram in Andhra Pradesh, southern peninsular region of India. The association of a bipartite begomovirus with the disease was done by sequence analyses of the cloned full-length genome. The full length genome sequences were submitted in NCBI GenBank with accession numbers MZ235792 (DNA-A) and MZ356197 (DNA-B). The sequence analysis of DNA-A of YMV-BG-BPT showed maximum of 99.12% similarity at nucleotide level with Mungbean yellow mosaic India virus (MYMIV) isolate reported from Tamil Nadu (KC911719), India which is also confirmed by clustering pattern in phylogenic analysis and DNA-B showed 95.79% with Mungbean yellow mosaic virus (MYMV) isolate reported from Tamil Nadu (KP319016) and 95.05% with MYMIV isolate reported from Karnataka (MT027037). The huge variation in DNA-B lead us to suspect a recombination in DNA-B, where a recombination event in the CR, region coding for nuclear shuttle protein and movement protein of DNA B was detected in which MYMV-BG-AP-IND (KF928962) and MYMIV-GG-CH-IND (MN020536) have been identified as major and minor parents, respectively.

Conclusion

Overall, the present study revealed occurrence of MYMIV with recombinant DNA B component in southern peneinsular India.

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Data availability

Raw data are available upon request

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Acknowledgements

Authors of the project acknowledged Acharya N.G. Ranga Agricultural University (ANGRAU), Indian Council of Agriculture Research (ICAR) and National Research Centre for Banana (NRCB) for financial support to successfully execute the research endeavors. Gratitude to National Research Centre for Banana (NRCB) Trichy, for constant technical assistance in the laboratory all through the experimentation period.

Funding

Funding for this Ph.D research work was provided by, Acharya N.G Ranga agricultural university, Lam, Guntur, Andhra Pradesh—522034. Indian Council of Agricultural Research (ICAR), New Delhi -110001.

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Authors and Affiliations

  1. Department of Plant Pathology, Agricultural College, Bapatla, Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh, India

    Vallabhaneni Tilak Chowdary & V. Manoj Kumar

  2. Department of Plant pathology, RARS, Anakapalle, Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh, India

    P. Kishore Varma

  3. Department of Crop Physiology, RARS, Lam, Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh, India

    B. Sreekanth

  4. Department of Statistics and Computer applications, Agricultural College, Bapatla, Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh, India

    V. Srinivas rao

  5. Molecular Virology Lab, Division of Crop Protection, ICAR National Research Centre for Banana, Thayanur Post, Tiruchirapalli, Tamil Nadu, India

    R. Selvarajan & V. Balasubramanian

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  1. Vallabhaneni Tilak Chowdary
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  2. V. Manoj Kumar
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Correspondence to Vallabhaneni Tilak Chowdary or R. Selvarajan.

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Chowdary, V.T., Manoj Kumar, V., Kishore Varma, P. et al. Characterization of Mungbean yellow mosaic India virus genome with a recombinant DNA-B in Southern Peninsular India.. Mol Biol Rep 49, 8587–8595 (2022). https://doi.org/10.1007/s11033-022-07691-9

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