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Aneuploids and its increment on diversity of Lippia alba polyploid complex: genetic aspects and origin

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Abstract

Background

Aneuploidy is associated with add or lack of individual chromosomes. The knowledge regarding aneuploidy is still rare in wild and tropical populations. Lippia alba is a tropical polyploid complex naturally formed, with 2x, 3x, 4x, 6x, and aneuploid individuals. The species presents pharmacological and medicinal importance, due to its essential oil compounds, which are related to the ploidal level. Considering the singularity of aneuploids emergence and stability, we proposed to investigate putative cytotypes involved in the aneuploids formation.

Methods and results

Molecular, cytogenetic, reproductive, and chemical approaches were adopted. The results showed that the aneuploids possibly have independent origin considering the genetic, chemical and karyotypical profiles. The chemical composition of aneuploids is related to genetic similarity. The aneuploid origin may involve 2x and 3x cytotypes being possible to rise four scenarios of crosses to explain that.

Conclusions

The results, in general, contribute to the comprehension of the origin of aneuploids and highlight the genetic profile of these accessions as a key element on the understanding of the chemical profile of L. alba accessions.

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Data availability

All generated sequences were deposited in GenBank (Online Resource 3).

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Acknowledgements

We would like to thank J. B. Pinheiro (Esalq-USP) and R. Vieira (Embrapa Cenargen) for providing some of the Lippia alba samples. We also would like to thank to Bioinformatic Laboratory – UFV for helping with data analysis using GENES software.

Funding

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (314443/2021-5); Fundação de Amparo à Pesquisa do Estado de Minas Gerais-Fapemig (CRA - RED-00053-16) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Capes.

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Authors and Affiliations

  1. Departamento de Biologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Minas Gerais, 36036-900, Brazil

    Juliana Mainenti Leal Lopes, Victória Rabelo Campos, Aryane Campos Reis, Elyabe Monteiro de Matos & Lyderson Facio Viccini

  2. Department of Genetic and Biotechnology, School of Life Science and Environment, University of Trás-os-Montes and Alto Douro, 5001-801, Vila Real, Portugal

    Juliana Mainenti Leal Lopes

  3. BioISI – Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, 1649-004, Lisbon, Portugal

    Juliana Mainenti Leal Lopes

  4. Embrapa Gado de Leite, Juiz de Fora, Minas Gerais, 36038-330, Brazil

    Ana Luisa Sousa Azevedo & Marco Antonio Machado

  5. Department of Chemistry, Federal University of Juiz de Fora, Juiz de Fora, Brazil

    Richard Michael Grazul

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  1. Juliana Mainenti Leal Lopes
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Contributions

JL, VC, AR, EM, and LV conceived and designed the experiments. VC and EM carried out the flow cytometry analyses. AR performed the cytogenetic analyses. JL, MM, and AA performed the molecular analyses. RG analyzed the essential oil profile. JL, VC, AR, EM, and LV contributed to the writing of the manuscript. LV revised the manuscript. All authors listed have made a substantial, direct and intellectual contribution to the work, and approved the manuscript.

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Correspondence to Lyderson Facio Viccini.

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Lopes, J.M.L., Campos, V.R., Reis, A.C. et al. Aneuploids and its increment on diversity of Lippia alba polyploid complex: genetic aspects and origin. Mol Biol Rep 49, 7743–7752 (2022). https://doi.org/10.1007/s11033-022-07599-4

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